Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0027819 (neuroblastoma)
 27,800 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The kinetics of mutual inhibition of taurine and hypotaurine uptake were studied using neuroblastoma C1300 cells as neuronal model. Hypotaurine and GABA inhibited taurine uptake competitively, increasing the apparent Km. High-affinity uptake of hypotaurine was completely abolished and the low-affinity component competitively inhibited by taurine. GABA affected noncompetitively low-affinity hypotaurine uptake, whereas the effect on high-affinity uptake was competitive, with an increase in the apparent Km. All structural analogues tested inhibited taurine and hypotaurine uptakes similarly. The most potent inhibitors were beta-alanine and 2-guanidinoethanesulphonic acid. The mutual inhibition and similar specificity profiles of taurine and hypotaurine uptakes showed that these amino acids employ a single transport system in neuroblastoma cells. Competitive inhibition by GABA of the high-affinity uptake of taurine and hypotaurine further suggests that also GABA uses the same carrier system.
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PMID:Taurine and hypotaurine transport by a single system in cultured neuroblastoma cells. 651 87

Uptake of [3H]taurine, [35S]hypotaurine, and [3H] gamma-aminobutyric acid (GABA) was studied in neuroblastoma C1300 cells in Krebs-Ringer-Hepes-glucose medium (pH 7.4). The uptakes consisted of nonsaturable penetration (taurine and hypotaurine) and two saturable transport components: high affinity for taurine, hypotaurine, and GABA and low affinity for hypotaurine and GABA. The affinity of the high-affinity uptake was highest for hypotaurine but the transport capacity was greatest for taurine. GABA uptake was almost abolished by taurine and hypotaurine. Hypotaurine also strongly inhibited taurine uptake, whereas GABA had only a moderate inhibitory effect on taurine and hypotaurine uptakes. The mutual inhibition suggests that these amino acids use the same transport sites when entering the cells.
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PMID:Taurine, hypotaurine, and GABA uptake by cultured neuroblastoma cells. 688 82