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Query: UMLS:C0027819 (neuroblastoma)
 27,800 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The nature of the restriction of herpes simplex virus replication in C 1300 neuroblastoma cells was studied. A low rate of adsorption was observed, probably due to the relatively few receptors for HSV on plasma membranes of C 1300 cells. The penetration rate of HSV to the nucleus was slow with an impaired processing of attached virus from plasma membrane to cell nucleus. Even at a high multiplicity of infection only a low percentage of the C 1300 neuroblastoma cells was permissively infected as determined by infectious centre assays. The yield of infectious HSV per virus-producing C 1300 cell was 1% of the yield from GMK control cells. The restriction in neuroblastoma cells of HSV infection could not be accounted for by sensitivity of cells to interferon or by an efficient induction of interferon. Evidence was obtained for the presence in C 1300 cells of an inhibitor of HSV replication not compatible with classical interferon. Observations on C 1300 cells maintaining many characteristics of differentiated neurons suggest that these cells may be useful as a model for studies on HSV-neuron interactions.
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PMID:Herpes simplex virus infection of in vitro cultured neuronal cells (mouse neuroblastoma C 1300 cells). 20 58

The synthesis of herpes simplex virus (HSV) in mouse neuroblastoma cells (NB, clone 41A3) is restricted. There was a disappearance of infectious virus upon serial passage of infected cells. NB cells treated with sodium-n-butyrate for 24 hr before infection synthesized 200-2000 times more HSV than untreated cells. Infectious center assays demonstrated that the number of cells capable of producing HSV was increased as a result of butyrate pretreatment. Although host protein synthesis was inhibited by HSV infection, viral-induced protein and DNA syntheses were not detected in the absence of butyrate. Cycloheximide blocked the induction of permissiveness by butyrate suggesting that a protein(s) was responsible for allowing HSV synthesis in NB cells. Regulatable host factors involved in HSV replication in neural cells can be studied in the system described.
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PMID:Butyrate-induced reversal of herpes simplex virus restriction in neuroblastoma cells. 302 2