Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0027819 (
neuroblastoma
)
27,800
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
As a nicotinamide adenine dinucleotide (NAD
+
)-dependent deacetylase, demalonylase, and desuccinylase,
sirtuin 5
(
SIRT5
) in host cells has been reportedly observed in the mitochondria, in the cytosol/cytoplasm or in the nucleus. Various functional roles of
SIRT5
have also been described in cellular metabolism, energy production, detoxification, oxidative stress, and apoptosis, but some of the reported results are seemingly inconsistent or even contradictory to one another. Using immunocytochemistry, molecular biology, gene transfection, and flow cytometry, we investigated the expression, subcellular distribution, and possible functional roles of
SIRT5
in regulating apoptosis and oxidative stress of cultured SH-EP
neuroblastoma
cells. Both endogenous and transfected exogenous
SIRT5
were observed in mitochondria of host SH-EP cells. Overexpression of
SIRT5
markedly protected SH-EP cells from apoptosis induced by staurosporine or by incubation in Hank's balanced salt solution.
SIRT5
also lowered the level of oxidative stress and countered the toxicity of hydrogen peroxide to SH-EP cells. It was suggested that the anti-apoptotic role of
SIRT5
was mediated, at least in part, by its anti-oxidative effect in SH-EP
neuroblastoma
cells although the involved molecular mechanisms remain to be elucidated in details.
...
PMID:Sirtuin 5 is Anti-apoptotic and Anti-oxidative in Cultured SH-EP Neuroblastoma Cells. 2757 43
