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Query: UMLS:C0026936 (Mycoplasma)
 14,761 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Three monoclonal antibodies elicited to NIH 3T3 cells transfected with DNA from a human pancreatic adenocarcinoma cell line recognized a novel ribonucleoprotein complex. Minimally, this ribonucleoprotein complex contained a Mr 240,000 protein (by sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and two RNA species with apparent sizes of 1.5 and 3.0 kilobases (by formaldehyde agarose gel electrophoresis). In addition to a cytoplasmic and nuclear subcellular localization, the RNA antigen was secreted from human tumor cell lines and NIH 3T3 cells transfected with pancreatic tumor DNA (inhibitable by monensin) and was apparently not a viral or Mycoplasma contaminant. The ribonucleoprotein antigen was detected in some normal tissues by immunoperoxidase but was not found in or secreted from in vitro cultured normal human fibroblasts, nontransfected or spontaneously transformed NIH 3T3 cells, or normal peripheral blood leukocytes.
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PMID:A novel ribonucleoprotein complex defined by monoclonal antibodies to NIH 3T3 cells transfected with human pancreatic adenocarcinoma DNA. 239 65

The attachment of Mycoplasma pulmonis m53 organisms to mouse and rat synovial cells was examined by using the organisms and the synovial cells treated in various ways. M. pulmonis treated with trypsin attached to the synovial cells, but the organisms treated with pronase, formaldehyde, glutaraldehyde, or heat did not. These findings suggest that the sites for binding M. pulmonis to the mouse and rat synovial cells are of polypeptide nature. Treatment of M. pulmonis with sialic acid and treatment of the synovial cell sheets with neuraminidase did not affect the attachment. The synovial cell surface for receptors M. pulmonis organisms would be different from those on respiratory cells or erythrocytes for M. pneumoniae or M. gallisepticum. Even nonviable organisms and M. pulmonis membranes attached to the mouse or rat synovial cells. The nature of the receptor of mouse synovial cells would be different from that of rat cells, since rat cells were affected by treatment with formaldehyde or glutaraldehyde, but mouse cells were not.
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PMID:Attachment of Mycoplasma pulmonis to rat and mouse synovial cells cultured in vitro. 408

Anderson, D. L. (University of Minnesota, Minneapolis), M. E. Pollock, and L. F. Brower. Morphology of Mycoplasma laidlawii type A. I. Comparison of electron microscopic counts with colony-forming units. J. Bacteriol. 90:1764-1767. 1965.-Cells of Mycoplasma laidlawii A grown in dialyzing flask cultures were counted with the electron microscope by use of a spray technique which deposited mixtures of polystyrene latex of known concentration and M. laidlawii in discrete droplet patterns on specimen films. Glutaraldehyde and formaldehyde were effective in preserving gross morphology of cells in spray preparations. The standard deviation of the mean ratio of latex-M. laidlawii was 5.5% when 2,000 total particles were counted in 18 droplet patterns. Microscopic counts resembled counts of colony-forming units (CFU) at various culture ages when cells larger than 0.25 mu were enumerated. If small bodies ranging from 0.1 to 0.25 mu in diameter were included in microscopic counts of cultures older than 70 hr, these counts exceeded the numbers of CFU by 4 to 10 times.
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PMID:Morphology of Mycoplasma laidlawii type A. I. Comparison of electron microscopic counts with colony-forming units. 532 28

Kim, Kwang S. (University of North Carolina, Chapel Hill), Wallace A. Clyde, Jr., and Floyd W. Denny. Physical properties of human mycoplasma species. J. Bacteriol. 92:214-219. 1966.-Studies were made of the comparative morphology and stability of five Mycoplasma species of human origin (M. hominis type 1, M. salivarium, M. fermentans, M. pneumoniae, M. pharyngis). Broth-cultivated organisms were examined by electron microscopy to determine their relative appearance after uniform processing, including fixation-drying with formaldehyde vapor. M. pneumoniae was characterized by the occurrence of 250- to 300-mmu spheres in clusters, and M. pharyngis by the appearance of filaments 120 mmu by 1.5 to 8 mu; the remaining species revealed a variety of structures, including spheres, rings, and short filaments. To complement these findings, the effect of physical stresses on viability of the mycoplasmas was measured by exposing the organisms to heat (in saline), osmotic variations (in sucrose), and sonic oscillation and repetitive freeze-thawing (in culture medium). M. pneumoniae was most resistant to heat, vibration, and freeze-thawing; M. pharyngis was most sensitive to heart and vibration, but was least affected by osmotic changes. The remaining organisms assumed intermediate positions. The type-related variations in relative morphology and stability suggest differing physical attributes of the mycoplasmas studied, supporting taxonomic differentiation of the five species based on metabolic and immunological criteria.
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PMID:Physical properties of human Mycoplasma species. 594 Dec 77

The cilia-stopping effect of mycoplasmas of human and various animal origin in mouse and chicken tracheal organ cultures was studied. From the results in mouse tracheal organ cultures, the mycoplasma strains tested were divided into three groups: Mycoplasma pulmonis m53, M pulmonis JB, M. pulmonis OK, M. mycoides subsp. Mycoides PGl and M. Gallisepticum S6 showed a strong cilia-stopping effect; M. pulmonis PG22, M. mycoides subsp. capri PG3, M. meleagridis 19729, M. neurolyticum Type A and M. arthritidis PG6 showed a mild effect; and M. pneumoniae FH, M. salivarium Hup, M. hominis type 1-C and M. orale N-C human origin and Acholeplasma laidlawii PG8 showed a weak effect. On the other hand, in chicken tracheal organ cultures, only M. gallisepticum S6 showed a strong effect, M. meleagridis 19729 was affected to a lesser degree, and the other mycoplasma strains showed a weak or no effect. The results indicate that some murine and poultry mycoplasmas showed a cilia-stopping tendency in mouse and chicken tracheal organ cultures, respectively, while human mycoplasmas showed weak or little effect in both organ cultures. In mouse tracheal organ cultures, M. pulmonis m53 treated with heat, trypsin or formaldehyde, and the sterile filtrate of an m53 broth culture showed no cilia-stopping effect. The relationship of the pathogenicity of mycoplasmas for their natural hosts to that for cultured respiratory cells is discussed.
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PMID:Comparison of ciliostasis by mycoplasmas in mouse and chicken tracheal organ cultures. 708 99

Adherence of Mycoplasma hyopneumoniae to the mucosa of the distal portion of the respiratory tract of swine is an important initial event in development of mycoplasmal pneumonia. A suitable in vitro model of adherence would be useful for investigation of mycoplasmal and host cell factors involved in this process. We have developed an adherence assay, using suspensions of porcine respiratory tract ciliated epithelial cells and M hyopneumoniae. Tracheal epithelial cells, collected by use of cytologic brushes, were mixed with broth cultures of M hyopneumoniae and the mixtures were incubated, diluted, vortexed, and sedimented. Pellets were spread on glass slides, stained with a fluorescent antibody against M hyopneumoniae, and evaluated by fluorescent microscopy. Fluorescence was observed principally among cilia on the ciliated tufts of epithelial cells. Only a few organisms were observed adhering on the nonciliated parts of ciliated cells or on other cell types. When mycoplasmas were preincubated with low dilutions of serum from swine convalescing from M hyopneumoniae disease, attachment was partially inhibited (P < 0.05). Significant inhibition of attachment was not observed when organisms were preincubated with higher dilutions of convalescent serum, with purified IgG from hyperimmune serum against M hyopneumoniae, or with low dilutions of lung lavage fluids (from convalescent swine) that contained specific IgA antibodies against M hyopneumoniae. Preincubation of the organisms with periodate and trypsin abolished attachment and formaldehyde decreased it (P < 0.05), whereas a variety of carbohydrates had no effect on attachment. Preincubation with dextran sulfate, ammonium sulfate, magnesium sulfate, and methionine reduced attachment (P < 0.05). Treatment of cell-Mycoplasma mixtures with the hydrophobic bond-breaking agent tetramethylurea, or incubation in absence of salt, or at low temperature also reduced attachment (P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Adherence of Mycoplasma hyopneumoniae to porcine ciliated respiratory tract cells. 821 93

The histologic changes in the respiratory tracts of chickens were evaluated after hatchery fumigation with 40% formaldehyde vapors and vaccination against infectious bronchitis virus with live attenuated vaccine (Massachusetts serotype). One-day-old chickens were housed in four isolation units in controlled environmental conditions, fed and watered ad libitum, and separated into four groups: 1) fumigated and vaccinated birds (FV group); 2) nonfumigated and vaccinated birds (NFV group); 3) fumigated and nonvaccinated birds (FNV group); and 4) control group (C group). All birds were tested to be free from Mycoplasma gallisepticum and Mycoplasma synoviae. After necropsy on the first, eighth, and twenty-sixth days after birth, samples from tracheal upper portion and lungs were conventionally processed for light, scanning, and transmission electron microscopy. Tissue response was monitored by microscopic examination of trachea and lung. On the first day of observation, fumigated and vaccinated birds (FV group) showed extensively damaged tracheal epithelium with exfoliated areas and some active glands with electrodense granules, and in the lung, the primary bronchi epithelium had disorganized cilia and abundant lymphocytes, with emphysematous areas in tertiary bronchus. On day 8 after vaccination, cubical and cylindrical tracheal cell proliferation was observed, and on day 26, ciliated columnar epithelium was almost regenerated with heterophil corion infiltration, and hyaline cartilage nodules appeared in parabronchi. The nonfumigated and vaccinated birds (NFV) revealed less injury on the epithelial surface and a more rapid response to epithelial regeneration than the in only fumigated animals (FNV). The control group did not show remarkable morphologic changes. Postvaccinal and fumigation effects on the upper respiratory tract were temporary, whereas in lungs, increased emphysema, cartilage nodules in the interchange zone, and general lymphocyte infiltration had caused intensive injury.
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PMID:Morphologic observations on respiratory tracts of chickens after hatchery infectious bronchitis vaccination and formaldehyde fumigation. 1100 97

Weibull, C. (Central Bacteriological Laboratory of Stockholm City, Stockholm, Sweden) and Britt-Marie Lundin. Morphology of pleuropneumonia-like organisms and bacterial L forms grown in liquid media. J. Bacteriol. 85:440-445. 1963.-Cells of Mycoplasma hominis, M. laidlawii, and two tissue-culture strains of pleuropneumonia-like organisms (PPLO) moving freely in liquid medium were photographed with an electronic flash as the light source. The photomicrographs thus obtained demonstrated that, in young cultures of high viability, the cells of these organisms were mainly filamentous or, in the case of M. laidlawii, coccoid. In old cultures of the same organisms containing predominantly nonviable cells, granular and vesicular elements were found. By the use of the same photographic technique, liquid cultures of a stable Proteus L form were studied. Although no filaments of uniform thickness were found, there were spherical bodies and some threadlike material connected with the spheres. When samples of PPLO cultures containing filamentous forms were transferred to agar blocks, the filaments were converted to more or less spherical bodies. This conversion could be prevented by fixing the PPLO with formaldehyde. The morphology of Proteus L forms was not noticeably altered by fixation with this reagent.
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PMID:Morphology of pleuropneumonia-like organisms and bacterial L forms grown in liquid media. 1399 17

Domermuth, C. H. (Statens Seruminstitut, Copenhagen, Denmark), M. Nielsen, E. A. Freundt, and A. Birch-Andersen. Gross morphology and ultrastructure of Mycoplasma gallisepticum. J. Bacteriol. 88:1428-1432. 1964.-The ultrastructure and gross morphology of Mycoplasma gallisepticum strains JA and W were studied with an electron microscope. Intact specimens were grown on Parlodion membranes, fixed with formaldehyde, and studied in situ. Sectioned specimens were grown on agar and were prepared for study by conventional sectioning techniques. Grossly, single and multiple (as many as four) protrusions were frequently observed extending from the surface of cells of the W strain. Single short protrusions extended from many of the cells of strain JA. In sectioned material, rows of what appeared to be developing elementary bodies (as many as four) were observed in cells of strain W, whereas, single, apparently developing elementary bodies were observed in strain JA. In both strains, these bodies were located within the protruding areas of the cell wall. The inclusion-containing portions of the cell appeared to be the protrusions which extended from the surface of the intact cell.
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PMID:GROSS MORPHOLOGY AND ULTRASTRUCTURE OF MYCOPLASMA GALLISEPTICUM. 1423 2

Harmonisation of standards can have benefits for both users and manufacturers of vaccines. It also helps regulatory authorities when products are assessed to have a uniform standard in different regions. The first priority for harmonisation has to be the elimination of duplicate testing for the same purpose, for example tests for safety, immunogenicity and batch potency. A further priority is to harmonise batch-testing requirements first, since duplication of testing affects production batches. Fish vaccines present particularities as far as harmonisation between regions is concerned because of geographical, climatic and other factors. A commitment from regulatory authorities and manufacturers is a precondition for harmonisation. The different parties should commit themselves to harmonisation within a reasonable time and to implementing their decisions promptly. When there is a commitment on the part of all participants to work together in developing common science-based regulatory standards, then the optimal conditions exist. Harmonisation is time-consuming and costly but in the end the benefits can no doubt exceed the investment. Harmonisation also needs a forum since the present terms of reference of regulatory and standardisation bodies do not cover this. The only forum at present is the International Cooperation on Harmonization of Technical Requirements for Registration of Veterinary Products (VICH), which is a tripartite activity involving the European Union, Japan and the U.S.A. Before deciding to propose inclusion in the VICH programme, a preliminary study should be carried out to assess the benefits expected, the obstacles to harmonisation and the feasibility of achieving a satisfactory result. In the framework of VICH, there have been some successful harmonisation projects for veterinary vaccines: residual formaldehyde determination, and residual moisture determination. A harmonised proposal for mycoplasma testing is now available for public comment. Work on extraneous agents testing for avian and mammalian viral vaccines is well advanced.
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PMID:European Pharmacopoeia (EP), USDA and MAFF standards--will they ever be harmonised under the VICH umbrella? 1596 85


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