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Query: UMLS:C0026936 (Mycoplasma)
 14,761 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Four monolayer mammalian cell lines were cured of Mycoplasma hyorhinis infections by cloning in microtiter dishes in the presence of tetracycline and kanamycin. During cloning, cultures were refed with fresh antibiotic containing medium every 2 or 3 d for 14 day and were then cultured without effective antibiotics for at least 21 d. From the four lines we recovered 29 clones, none of which were infected after treatment as judged by the lack of extranuclear fluorescence after staining with the fluorochrome Hoechst 33258, and by normal autoradiographic labeling of the cells by tritiated nucleosides. One clone from each line was tested further by attempted culture of mycoplasmas and was also judged to be uninfected. Infection has not reappeared in any of the clones after extensive culture in the absence of the effective antibiotics.
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PMID:Elimination of Mycoplasma hyorhinis infections from four cell lines. 731 31

Leghorn pullets free of Mycoplasma gallisepticum (MG) were infected with the F strain of MG at approximately 18 weeks of age. At various times up to 27 weeks postinfection, infected chickens were placed in a pen with uninfected controls. Infected chickens remained tracheal carriers up to 49 weeks postinfection. Infection was readily transmitted to penmates during the first 4 weeks postinfection; in most instances from 6 through 27 weeks postinfection, transmission to penmates became progressively slower. Five groups of broiler chickens were reared in the same house as the Leghorn chickens up to 8 weeks of age. Only one of the 5 groups became infected. Birds in this group were reared in a pen adjacent to infected pullets from 1 day of age and were apparently not infected until they were nearly 8 weeks old. At no time did transmission occur between groups separated by an aisle or empty pen.
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PMID:Transmissibility of the F strain of Mycoplasma gallisepticum in leghorn chickens. 733 4

Infection, lesions and clinical significance of Acheloplasmas, Mycoplasma bovis and Mycoplasma bovigenitalium in genital disease of cattle are described. A more detailed account is given of ureaplasma infections. Acute and chronic forms of granular vulvitis in both field and experimental disease are described as well as the role of the organism in abortion. Recovery rates of ureaplasma and mycoplasma from semen and preputial washings in bulls are outlined and their significance in disease is discussed. There are problems in differentiating pathogenic from nonpathogenic isolates. Methods are being developed to treat semen for these organisms. This paper provides a concise summary of clinical and microbiological aspects of bovine genital mycoplasmosis.
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PMID:Bovine genital mycoplasmosis. 733 8

A retrospective survey of 81 patients with serologically proven Mycoplasma pneumoniae infection revealed that 72% received two or more different antibiotics during their illness, ampicillin and other penicillins being most frequently used. Fifteen (18.5%) patients had a skin rash, most commonly an erythematous maculopapular rash. It is suggested that such rashes may be due to antibiotics and, unlike mucocutaneous vesicular rashes, are probably not a useful indication of mycoplasmal pneumonia.
Infection 1980
PMID:Mycoplasma pneumoniae infection, antibiotics and exanthema. 741 76

A monoclonal blocking enzyme-linked immunosorbent assay (blocking-ELISA) was developed to detect antibodies to Mycoplasma gallisepticum (MG) in poultry sera with the help of a peroxidase-labeled monoclonal antibody (MAb) recognizing an epitope of a 56-kilodalton polypeptide (p56) of MG. Immunoglobulins from undiluted MG-positive sera prevent the MAb conjugate from attaching to its specific binding site on p56, which results in no color development. The opposite result--a strong color reaction--was obtained after incubation with MG-negative sera (or when no serum was added before the MAb conjugate). Results were expressed in percent inhibition or ELISA titers. The blocking-ELISA detected 84.7% positive chickens in an experimentally infected flock and 72.6% of chickens in naturally infected flocks, whereas the hemagglutination-inhibition (HI) test was positive only with 68.4% and 48.6% of these serum samples, respectively. All HI-positive serum samples reacted positively in blocking-ELISA. Of sera negative by the HI test, 51.6% and 46.8% proved to be positive when examined with the blocking-ELISA. Overall agreement between the ELISA and HI test was 76.8%. Infection with closely related M. synoviae did not induce any false-positive reactions in blocking-ELISA. There was a strong positive correlation between HI and blocking-ELISA titers (r = 0.83).
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PMID:Evaluation of a monoclonal blocking enzyme-linked immunosorbent assay for the detection of Mycoplasma gallisepticum-specific antibodies. 750 18

Infection of tracheal organ cultures with Mycoplasma dispar results in degeneration of respiratory epithelial cells with loss of ciliary activity. To assess the effect of these changes on the clearance of bacteria from the respiratory tract, the tracheobronchial clearance of a suspension of Serratia marcescens was determined in calves before and after infection with M. dispar. Tracheobronchial samples were obtained at various times after deposition of the marker bacteria. Clearance of S. marcescens was significant by 3 h after inoculation and only few colonies were detected 4 h after challenge. By contrast, in the same animals 5 days after intratracheal exposure to M. dispar, clearance of S. marcescens did not decrease significantly 4 h after inoculation. Histopathological and scanning electron microscopy (SEM) studies of infected lungs revealed that, as a result of infection with M. dispar, areas of degeneration and destruction of the respiratory ciliated epithelium occur in intermediate and small airways. These lesions may be responsible for the altered clearance observed in these mycoplasma-exposed calves.
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PMID:Impaired tracheobronchial clearance of bacteria in calves infected with Mycoplasma dispar. 770 60

We investigated 56 boys and 30 girls between the ages of 1 month and 15 years with acute respiratory infections for antibodies to Chlamydia pneumoniae and Mycoplasma pneumoniae. Antibodies were only occasionally found in children less than 10 years of age (4% of boys (1/23) and 5% of girls (1/19). In the age group 10-12 years, antibodies were detected in 55% of boys (4/9) but not in the 2 girls investigated. In the age group 13-15 years, antibodies were detected in 58% of boys (14/24) and in 33% of girls (3/9). Infection caused by M. pneumoniae was found in 13% of boys in the oldest age group.
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PMID:Prevalence of specific antibodies to Chlamydia pneumoniae in children with acute respiratory infections. 784 26

In this study, the prevalence of mycoplasmas in peripheral blood mononuclear cells (PBMC) from HIV-infected individuals was investigated using a mycoplasma genus-specific PCR assay. No mycoplasmas were detected in the PBMC samples from any of the 25 HIV-infected individuals (CDC 2, n = 8; CDC 3, n = 2; CDC 4, n = 15) or ten HIV-seronegative controls. As an internal control, HIV specific sequences were detected in the samples from all HIV-seropositives. These negative results do not support a suggested role of mycoplasmas as co-factor in the progression of HIV infection towards AIDS.
Infection
PMID:No evidence of mycoplasmas in peripheral blood mononuclear cell fraction of HIV-infected patients. 792 18

An invertible DNA element of 6.8 kb, designated the hsd1 locus, was identified in the chromosome of Mycoplasma pulmonis. Infection of host cells with mycoplasma virus P1 revealed that the organism's restriction and modification (R-M) properties are controlled by inversion of hsd1. The nucleotide sequence of hsd1 revealed several genes, the predicted amino acids of which bear striking similarity to the subunits of the type I R-M enzymes previously found only in enteric bacteria.
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PMID:Regulation of a restriction and modification system via DNA inversion in Mycoplasma pulmonis. 793 78

To study the time-course of infectious asthma, we retrospectively examined FEV1 from 5 days before to 10 days after the onset of illness in 31 asthmatic children (20 boys and 11 girls), aged 8 to 12 years. Infections were confirmed by a rise of at least fourfold in serum compliment fixation titers (respiratory syncytial virus, adenovirus, and Mycoplasma pneumoniae) and hemoagglutination inhibition titers (parainfluenza virus types 1, 2, and 3). All the patients had 20 percent or more fall in FEV1 from baseline value during acute phase, but were clinically tolerable and required minimum or no bronchodilators. Regardless of infectious agent, FEV1 began to fall on the first disease day or the previous day, and deteriorate for the first few days. Mean(SD) maximum fall in FEV1 ranged from 39(12) percent to 45(20) percent. Thereafter, FEV1 began to improve and returned to the preillness level by the seventh to tenth day. These results suggest that progressive bronchial obstruction may be inevitable during the acute stage of any infectious asthma.
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PMID:Progressive bronchial obstruction during the acute stage of respiratory tract infection in asthmatic children. 802 Feb 53


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