Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0026918 (Mycobacterium)
 52,428 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Formate dehydrogenase activity (EC 1.2.1.2) has been demonstrated in cell-free preparations of Mycobacterium phlei by following the reduction of 2,6 dichlorophenolindophenol. thiazolyl blue tetrazolium, or equine cytochrome c. The reduction of equine cytochrome c was inhibited by 2-heptyl-4-hydroxyquinoline-N-oxide. Neither nicotinamide adenine dinucleotide nor nicotinamide adenine dinucleotide phosphate were reduced by this formate dehydrogenase. The enzyme was constitutive and associated with the particular fraction. The greatest level of activity was observed at pH 9.0, with 8 mM formate, and with extracts of cells taken from the log phase of growth. Formaldehyde, hypophosphite, nitrate, and bicarbonate all inhibited the oxidation of formate.
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PMID:Nicotinamide adenine dinucleotide -- independent formate dehydrogenase in Mycobacterium phlei. 1 20

The activities of the nitrate reductase enzyme of Mycobacterium tuberculosis, M. bovis, and of BCG were assayed with and without addition of electron donors. M. tuberculosis always reduced nitrate; M. bovis did so only in the presence of electron donors, and BCG did not show enzymatic activity.
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PMID:Nitrate reductase activity of Mycobacterium tuberculosis and Mycobacterium bovis in the presence of electron donors. 9 6

Two tuberculin-positive baboons in a primate colony were found to have grossly visible tuberculous lesions in the liver, spleen, lung, and mediastinal lymph nodes on necropsy. Results of histopathologic examination of the tissues showed granulomas with Langhans giant cells. An acid-fast organism was isolated from tissues of each baboon; the isolates were identified as Mycobacterium bovis by being negative for niacin production and nitrate reduction and by their susceptibility to thiophen-2-carbosylic acid hydrazide and to 5% glycerol.
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PMID:Mycobacterium bovis infection in baboons (Papio papio). 32 36

It was the aim of the present communication to find a simple test for a reliable discrimination of Mycobacterium bovis BCG from Mycobacterium tuberculosis. A total of 26 BCG strains, out of them 10 Czechoslovak strains (2 lyophilized cultures of BCG of different batch, 6 strains isolated from abscesses of children after BCG-vaccination and 2 strains from fatal cases after BCG-vaccination) and 16 strains obtained from foreign laboratories, were used. Of the tested characteristics a combination of 3 tests, sensitivity to 1 microgram of 2-thiophene carbonylhydrazide (TCH), activity of 3 acylamidases (urease, nicotinamidase and pyrazinamidase) and a quantitative nitrate test, was found to be most advantageous. The Czechoslovak strains of Mycobacterium bovis BCG were fully sensitive to TCH, of the 3 acylamidases mentioned above only urease was positive and nitrate was reduced only little or not at all. On the other hand, strains of Mycobacterium tuberculosis were always resistant to TCH, had positive urease, nicotinamidase and pyrazinamidase and reduced nitrate very intensively.
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PMID:Cytochemical and biological properties of Mycobacterium bovis BCG. 33 Mar 64

A total of 24 strains of the Mycobacterium fortuitum complex were tested for susceptibility to antimicrobial agents by the disk diffusion and agar dilution techniques. By comparing zones of inhibition obtained with the disk diffusion technique with results of minimal inhibitory concentration determinations, it was shown that disk diffusion results could predict in vitro susceptibility to selected antimicrobial agents. All of 17 strains of M. fortuitum were susceptible to </=1 mug of amikacin per ml. The corresponding average zone of inhibition around a 10-mug amikacin disk was 37 mm. Seven M. chelonei strains were more resistant to amikacin, with minimal inhibitory concentrations ranging from 1 to 32 mug/ml, and the corresponding average zone size was 21 mm. Susceptibility of both M. fortuitum and M. chelonei to tetracycline was variable and none of the M. chelonei strains was inhibited by polymyxin B, whereas M. fortuitum strains consistently had zones of inhibition around the polymyxin disk. It appears that identification to species of the M. fortuitum complex may be of importance with regard to antibiotic susceptibility. Separation of M. fortuitum and M. chelonei was readily accomplished in the present study by the nitrate reduction and 3-day arylsulfatase tests.
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PMID:Antimicrobial susceptibility testing of Mycobacterium fortuitum complex. 47 59

The philosophy of the recently proposed "Levels of Laboratory Service" program, which will be so vital to the conduct of a successful outpatient tuberculosis treatment and control program, is presented. The hallmark of this program is the decentralization of the diagnostic/monitoring services as they involve laboratory participation. In the long run this could mean more efficient operation, more reliable reporting, and probably less work for the participating laboratories. The greater emphasis on smear examination (Level I) as a monitoring tool will mean fewer cultures, thereby lessening the load for those laboratories that once went through countless clinically requested exercises of repetitively proving by culture the existence of M. tuberculosis in a given patient. Doubtless, the bulk of the work will be conducted in Level II laboratories; but here, too, identification of the most easily defined pathogen, M. tuberculosis, will minimize the over-all workload for these investigators while decreasing their concern about mycobacteria other than tubercle bacilli. Expertise gained in frequent repetitions of a limited number of tests (niacin, nitrate reduction, and pH 7/68 degrees C catalase) will ensure reliable speciation of the clinically most important Mycobacterium. The work of Level III laboratories should eventually be reduced primarily to organisms other than M. tuberculosis, thereby ensuring that a number of highly competent reference institutions will not only attain proficiency in taxonomic aspects of mycobacteria, but will also reflect the regional picture of the changing patterns in mycobacterial pathogens of man. Participation of laboratories in proficiency testing programs will encourage top-level performance in all areas. Additionally, such testing programs will serve a teaching role; a laboratory need not feel "locked in" at a given service level, but may increase its proficiency and move up a step in terms of the service it provides. In contrast, no laboratory need feel compelled to increase its activities; if daily workloads limit the extent of their involvement with mycobacteria, these laboratories can be confident that other institutions are providing needed services. The success of the entire "Levels of Laboratory Service" program depends on the recognition by individual laboratories of their own workload limitation, the directed motivation of personnel, and the maintenance of a free and open pipeline of communication to laboratories at the next higher level of service.
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PMID:Laboratory services for mycobacterial diseases. 81 99

A medium has been selected for providing the maximum synthesis (7-8 g/litre) of a free exocellular polysaccharide by Mycobacterium lacticolum 121. The following concentrations of the components are optimal for the biosynthesis: hexadecane, 5.0-6.5% (by volume); NaNO3, 2.0-4.0 g/litre; Na2HPO4-12H2O, 1.0-1.5 g/litre; KCl, 1.0-1.5 g/litre. An increase in the concentration of sodium nitrate and phosphate over 4.0 and 1.5 g/litre, respectively, favours growth of the cells but inhibits biosynthesis of the polysaccharide. Concentrations of hexadecane and potassium chloride over 6.5% and 1.5 g/litre, respectively, have no effect of the culture and yield of the polysaccharide.
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PMID:[Optimization of the culture medium and role of its components in the biosynthesis of exopolysaccharides by Mycobacterium lacticolum]. 100 65

The effect of 12 sources of nitrogen on growth and cholesterine-decomposing activity was studied with Mycobacterium rubrum and Achromobacter candicans. The yield of biomass and the rate of cholesterine decomposition depended on the source of nitrogen and its concentration in the medium. The highest specific activity of the enzyme decomposing cholesterine was found during growth of the cultures on media containing reduced forms of nitrogen. The activity of the enzyme of Mycobacterium rubrum was by 25% higher than that of Achromobacter candicans. The optimum conditions for the production of the enzyme by Mycobacterium rubrum were on media containing 1.0 g of asparagine or 5.0 g of ammonium nitrate per one litre, and for the enzyme production by Achromobacter candicans, on media containing 5.0 g of ammonium phosphate per one litre.
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PMID:[Effect of nitrogen sources on growth and cholesterol decomposing activity of Mycobacterium rubrum and Achromobacter canadicans]. 101 55

Strains of scotochromogenic mycobacteria were studied by using numerical taxonomy methods in an attempt to more clearly define Mycobacterium szulgai and to find tests useful in identifying the species. In this study all strains of M. szulgai were strong reducers of nitrate, were slow in hydrolyzing Tween 80, and gave a high semiquantitative catalase reaction. Results obtained indicate that the use of increased pigmentation after 1 h of light exposure at 25 C and that the use of arylsulfatase activity are of questionable diagnostic value in separating the species from other scotochrompgenic mycobacteria.
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PMID:Differential identification of Mycobaterium szulgai and other scotochromogenic mycobateria. 126 53

A rapid growing acid-fast organism was isolated from the blood of a borderline leprosy patient. The isolate appeared to be close to Mycobacterium cheloni group of organisms but showed globi, cigar shaped bundles and was positive for DOPA-oxidase. Catalase, iron uptake, sodium chloride tolerance, tellurite reduction, Tween 80 hydrolysis and pyridine extraction tests were also positive. The 3-days arylsulphatase test and nitrate reduction test were negative.
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PMID:Isolation of a DOPA positive rapid growing mycobacterium from blood of a leprosy patient. 157 5


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