Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0026918 (Mycobacterium)
 52,428 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A study has been made of the activity of interleukin 1 (IL-1) and prostaglandins (PGs) in the culture supernatants from unstimulated and lipopolysaccharide (LPS)-stimulated mycobacteria-induced granuloma cells. Both epithelioid cells from bacillus Calmette-Guerin (BCG)-induced granulomas and macrophages from Mycobacterium leprae-induced granulomas, separated on a fluorescence-activated cell sorter using monoclonal antibody specific to guinea pig macrophages, spontaneously secreted low levels of IL-1 (assayed by thymocyte comitogenic and fibroblast mitogenic activities) into culture supernatants. However, culture supernatants from LPS-stimulated epithelioid cells showed significantly higher IL-1 activity than those from unstimulated cells. In contrast, LPS stimulation of M. leprae granuloma macrophages failed to enhance IL-1 production. Nevertheless, IL-1 activity in the culture supernatants from stimulated mycobacterial granuloma cells of both types was much lower than that from LPS-stimulated peritoneal exudate macrophage culture supernatants. There was no detectable amount of prostaglandin E2 (PGE2) in the culture supernatants from both unstimulated and LPS-stimulated BCG- and M. leprae-induced granuloma cells in comparison to much higher levels of PGE2 produced by unstimulated (0.28-6.2 ng/ml) or LPS-stimulated (greater than 15 ng/ml) peritoneal exudate macrophages. However, BCG granuloma cells either secreted prostaglandin F2 alpha (PGF2 alpha) spontaneously or produced comparable levels of PGF2 alpha to those from peritoneal exudate macrophages on stimulation, while M. leprae granuloma macrophages produced much lower levels of PGF2 alpha.
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PMID:Interleukin 1 and prostaglandin production by cells of the mononuclear phagocyte system isolated from mycobacterial granulomas. 349 93

Two distinct types of granulomas were produced in the draining lymph nodes by immunizing guinea pigs with Mycobacterium bovis BCG or Mycobacterium leprae, as reported earlier (Narayanan et al., J. Pathol. 134:253-265, 1981). In the BCG-induced granuloma there is successful containment, killing, and degradation of the organisms with the presence of epithelioid cells and fibrosis. M. leprae, on the other hand, induces a granuloma where there is an absence of organization of the cells, failure to completely degrade the organisms, absence of epithelioid cells, and minimal fibrosis. By using a macrophage-specific monoclonal antibody and an anti-Ia monoclonal antibody and applying the immunoperoxidase, immunofluorescence, and fluorescence-activated cell sorter analysis techniques, the epithelioid cells of the BCG granuloma were found to have macrophage-specific antigen, but not detectable amounts of Ia antigen. This suggests that these cells have a close relationship to other cells of the mononuclear phagocyte series with which they share a common antigen. The absence of Ia antigen, on the other hand, suggests that epithelioid cells may not be involved in antigen presentation or other accessory cell functions where the presence of Ia antigen is crucial. The macrophages in the M. leprae-induced granuloma expressed both macrophage-specific and Ia antigens.
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PMID:Analysis of cells of the mononuclear phagocyte series in experimental mycobacterial granulomas by monoclonal antibodies. 633

Recent studies in man and animal models have demonstrated that TCR-gamma delta-bearing T cells (gamma delta T cells) are activated by mycobacteria and accumulate in the sites of mycobacterial infection. Although the function of gamma delta T cells remains unclear, some data suggest a potential role for these cells in the granulomatous immune response. To address the presence of gamma delta T cells within the BCG granulomas, we have characterized the TCR phenotype of T-lymphocytes present in the BCG granulomatous lesion immunohistochemically using a monoclonal antibody to TCR delta 1 and others. Fairly large numbers of gamma delta T cells were located at the periphery of the BCG granulomas without necrosis and most of them also expressed CD8. However, gamma delta T cells were rarely present in the granulomas with central caseous necrosis, calcification and fibrotic changes. With these results, it might be speculated that the CD8+ gamma delta T lymphocytes participate in the BCG granuloma formation mainly in the early stage.
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PMID:Gamma/delta T lymphocytes in the BCG granulomatous lesions. 899 64