Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0026850 (
muscular dystrophy
)
5,870
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Satellite cells (SC) are muscle stem cells that can regenerate adult muscles upon injury. Most SC originate from PAX7
+
myogenic precursors set aside during development. Although myogenesis has been studied in mouse and chicken embryos, little is known about human muscle development. Here, we report the generation of human induced pluripotent stem cell (iPSC) reporter lines in which fluorescent proteins have been introduced into the
PAX7
and
MYOG
loci. We use single cell RNA sequencing to analyze the developmental trajectory of the iPSC-derived PAX7
+
myogenic precursors. We show that the PAX7
+
cells generated in culture can produce myofibers and self-renew
in vitro
and
in vivo
Together, we demonstrate that cells exhibiting characteristics of human fetal satellite cells can be produced
in vitro
from iPSC, opening interesting avenues for
muscular dystrophy
cell therapy. This work provides significant insights into the development of the human myogenic lineage.
...
PMID:Differentiation of the human PAX7-positive myogenic precursors/satellite cell lineage
in vitro
. 3254 Oct 4
