Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0026764 (multiple myeloma)
 36,148 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Hebdomadis is one of the major serogroups found in China. In Sichuan, this serogroup appears to have a close relationship with local outbreak of leptospirosis. BALB/c mice were immunized intrasplenically with outer envelope of serogroup Hendomadis serovar hebdomadis strain 245. Spleen cells were fused with SP2/0 myeloma cells, two monoclonal antibodies Af2 and Bb2 were produced by hybridoma technique. McAb Af2 and McAb Bb2 were identified to be IgG1 and IgG3 by immunodiffusion respectively. Specificities of these two McAbs were determined by MAT; both reacted to 8 serovars (hebdomadis, nona, kambale, kremastos, worsfoldi, jules, maruborincana) of Hebdomadis serogroup. The agglutination titres of McAb Af2 and McAb Bb2 were 1:640-1:2,500,000 and 1:320-1:2,500,000, respectively. The two McAbs did not agglutinate with serovar kabura of Hebdomadis serogroup, they did not agglutinate with 11 serovars of Sejroe serogroup, 4 serovars of Mini serogroup, 18 representive serovars of L. interrogans in 18 serogroups, L. biflexa strain Patoc I and Leptonema illini. So it was found that McAb Af2 and McAb Bb2 showed partial serogroup specificity for Hebdomadis by agglutination.
Hua Xi Yi Ke Da Xue Xue Bao 1992 Sep
PMID:[The production and identification of partial serogroup--specific monoclonal antibodies against leptospires hebdomadis serogroup]. 128 91

Spleen cells of BALB/c mice immunized with whole Leptospira interrogans serogroup Australis serovar australis strain 620 were fused with myeloma cells line SP2/0. Specificities of four McAbs determined by MAT. 2E1 McAb (IgG3) reacted with 11 serovars, of the Australis serogroup, but did not react with 22 representative serovars of L. interrogans in 20 serogroups, L. biflexa strain patoc I and Leptonema illini strain 3055. 2E1 McAb showed serogroup specificity for Australis by agglutination and the other 3 McAbs showed partial serogroup specificity. We compared the outer envelope (OE) protein profiles of serovar australis strain 620 with those of two pathogenic L. interrogans serovar lai strain 601 and serovar hebdomadis strain 156 by SDS-PAGE. 63kd protein profile was only found in the OE of strain 620, and the quantity of 42kd protein of strain 620 was greater than that of strain 601 and 156. The immunoblotting revealed that 2E1 McAb reacted with a 34kd band in the OE preparation of serovar australis strain 620, but did not react with that of other two L. interrogans. 2E1 McAb also did not react with OE of non-pathogenic leptospires. It was suggested that 34kd protein might contain the antigenic determinants which were shared by leptospires of Australis serogroup.
Hua Xi Yi Ke Da Xue Xue Bao 1990 Sep
PMID:[Study on the production, identification of serogroup-specific monoclonal antibodies against leptospires of Australis serogroup and detection of its antigen]. 170 13

Using Ficoll-Hypaque gradient centrifugation, we have successfully obtained the neutrophils from normal human donors, which can be used as particle antigens for immunizing BALB/c mice. Hybridomas were produced through the fusion of SP2/0 myeloma cells and splenocytes from immunized BALB/c mice. The ratio of fusion was 1:5. The cells were fused with 30% polyethylene glycol (MW 4000). The rate of fusion was 90%. The antibody producing colonies against the membrane of neutrophils in HAT medium were selected by indirect immunofluorescence assay. Antibody positive rate was 60% (102/170). Limiting dilution was used for colonization of the antibody-producing hybridoma cells. After colonization for three times, one hybridoma cell line was obtained, which could inhibit complement-mediated phagocytosis. Culture supernatant was used against class- and subclass- specific rabbit antisera (rabbit anti-mouse IgM, IgG, IgG1, IgGd22, IgG2b, IgG3) in an agar immunodiffusion system, it was shown to be IgG22.
Hua Xi Yi Ke Da Xue Xue Bao 1991 Mar
PMID:[Screening and identification of monoclonal antibodies against human neutrophil and detection of inhibiting opsonic phagocytic activity]. 177 29

The fusion of peripheral B lymphocytes from human immunized with P. aeruginosa polyvalent vaccine and mouse myeloma cell line SP2/0 was successfully performed. The rate of fusion was 74%(71/96) and the positive rate of antibody was 19.7%. Two hybridoma cell lines (A3 and F8) secreting McAb against P. aeruginosa were obtained after three times cloning by limiting dilution. The human chromosomes together with mouse chromosomes were discovered in karyotype assay of the hybrids. A3 and F8McAbs were human IgG by class determination. These MrcAbs could recognize 43 kd and 36 kd MW specific components of P. aeruginosa antigen by enzyme linked immuno-transfer blot technique.
Hua Xi Yi Ke Da Xue Xue Bao 1991 Mar
PMID:[The production of human monoclonal antibodies against Pseudomonas aeruginosa by human-mouse hybridoma technique]. 177 30

We reported the production of monoclonal antibodies (McAbs) against chorionic gonadotropin hormone (CG) receptor by fusing spleen cells of BALB/c mice which had been immunized by purified bacteria (Pseudomonas maltophilia) CG receptor with mouse myeloma line SP2/0. Four hybridoma cell lines secreting CG receptor McAbs were obtained (ED490 DG390, AB890 and GE590). The titers of specific antibodies of both mice ascites and culture supernatant were 10(-2)-10(-6) and 1-10(-2) respectively, by solid phase ELISA. Double-Immunodiffusion test showed that the McAb GE590 was IgG1, and the McAbs ED490, DG390 and AB890 were IgG2b Immunoprecipitation indicated that 125I-HCG could bind the HCG receptor which had reacted with McAbs ED490, AB890 and DG390, suggesting that they may recognize the receptor with different antigenic determinant. Interaction of McAb GE590 with the receptor showed that the increased concentration of GE590 was in inverse proportion to the amount of 125I-HCG binding receptor, indicating that both the McAb and 125I-HCG could recognize a common site of receptor and that increased concentration of McAb GE590 may induce some change in conformation and structure of the receptor. Our study suggested that these McAbs may be used for studying structure of CG receptor.
Hua Xi Yi Ke Da Xue Xue Bao 1991 Sep
PMID:[Preparation of monoclonal antibodies against chorionic gonadotropin receptor and study of its characteristics]. 181 14

BALB/c mice were immunized intraperitoneally with outer envelopes of serogroup icterohaemorrhagiae lai serovar strain 017 leptospires. Monoclonal antibodies against outer envelopes (IgG, agglutinating titre 1:25,600) were produced by hybridoma technique. The monoclonal antibodies ascites (diluted 1:100) 1 ml administered intraperitoneally 1 hour before the intraperitoneal injection of 2 x 10(8) leptospires of strain 017 and the subsequent daily administration of McAb in similar doses for five days protected 80% of guinea pigs. Survival rates of three control groups which received physiological saline, ascites of BALB/c mouse myeloma cell lines SP2/0, and monoclonal antibodies against Pseudomonas aeruginosa in place of monoclonal antibodies against outer envelopes of strain 017 leptospires were 10%, 20% and 10% respectively. When killed 20 days after challenge, guinea pigs of experiment group were normal at autopsy. Old pulmonary haemorrhage were present in the animals of three control groups. Passive immunoprotection experiments have demonstrated immunoprotection of monoclonal antibodies against outer envelopes of strain 017 leptospires. It will be valuable for separating protective antigen fraction of outer envelopes and studying new vaccine of leptospira.
Hua Xi Yi Ke Da Xue Xue Bao 1990 Sep
PMID:[Investigation on the immunoprotection of monoclonal antibodies against outer envelopes of serogroup Icterohaemorrhagiae serovar lai strain 017 leptospires]. 209 58

BALB/c mice were infected per os with the infective larvae (L1) of Trichinella spiralis, and challenged by injecting 0.2ml soluble L1 antigen 3 days before cell fusion. SP2/0 myeloma and immune spleen cells were fused at the ratio of 1:5 in the presence 30%PEG (MW 4,000). The fusion rate and antibody positive rate were 92.1% and 16.6% respectively using ELISA. Among these the chromosomes of 4 strains were 2n greater than 100 and that of the SP2/0 myeloma cells 2n less than or equal to 70. The titer of McAb in the ascites was found to be 1/640 or 1/1,280. Eight strains were of IgG1, 1 IgG2a and 3 IgM in an agar system. Three strains of McAbs, which could recognize specifically the L1 antigen fractions by immuno-blotting technique, were used as probes to localize the target antigens in sections of T. spiralis L1. Staining was performed using an indirect technique consisting of goat anti-mouse IgG-conjugated horseradish peroxidase on de-paraffin sections, and substrate DAB. The results revealed that the antigens reacted most strongly with the specific McAbs were located in the cuticle and stichosome, followed by the lining of gut. Among the 3 strains of McAbs used, 2E5F11A5 reacted most strongly with the antigen, followed by 2E5E9E4. 2E5E9G5 was the weakest.
Hua Xi Yi Ke Da Xue Xue Bao 1990 Mar
PMID:[Preparation of monoclonal antibodies against Trichinella spiralis and localization of antigens]. 236 31

BALB/c mice were immunized with the purified p30 antigen. SP2/0 myeloma cells and immune spleen cells were fused with 50% PEG (Sigma, MW 3,350-4,000). The cell fusion rate was 93.95%, and the antibody producing rate 21.01%. The technique of limiting dilution was used for cloning of the hybridoma cells. Two hybridoma cell lines E8 and G1 secreting McAb against p30 antigen were obtained. The number of chromosome of both E8 and G1 cell lines was 98.7 +/- 6.54 and 97.7 +/- 7.77, respectively. Results of the PAGE of the ascites generated by the hybridoma cell lines E8 and G1 showed a thick protein band at the gamma-region which was absent from the ascites generated by the SP 2/0 myeloma cells. The immunoglobulin of the McAb E8 and G1 belonged to IgG2 subclass. The results of the immunohistochemical method using the horseradish peroxidase conjugated antibody showed that both E8 and G1 McAb only reacted with epithelial cells of the normal human prostatic glands and their ducts, but did not cross-react with other thirty-four different kinds of normal human tissues. The results of inhibiting ELISA test showed that both E8 and G1 McAb were only inhibited by the human seminal plasma and p30, weakly inhibited by the adult male's urine, but were not inhibited by other eight different kinds of human body fluids and secretions as well as semen from eight different species of animals. It was concluded that McAb of E8 and G1 were organ and species specific.(ABSTRACT TRUNCATED AT 250 WORDS)
Hua Xi Yi Ke Da Xue Xue Bao 1990 Mar
PMID:[Establishment of hybridoma cell lines producing monoclonal antibodies against-p30 antigen]. 236 36

Three McAb were produced against an outer envelope preparation from Leptospira, interrogans, serovar Lai by fusion of SP2/0 myeloma cells with immune BALB/c mice spleen cells. The fusion rate was 96% and the antibody positive rate was 50%. One of the hybridomas, E4B11C9, reacted with 13 of the 13 serovars of the Icterohaemorrhagiae serogroup in microscopic agglutination test (MAT) but did not react with the 18 representative serovars of L. interrogans and L. biflexa serovar patoc and Leptonema illini. For all non-reactive serovars the MAT titres were greater than 1:25. The McAb, E4B7G5, reacted similarly with all serovars except smithi and tonkini. E4B7D4 reacted also similarly with all serovars except serovars birkini, ndambari, bogvere, smithi and tonkini. Therefore, 3 McAb showed serogroup specificity and partial serogroup specificity by agglutination. The agglutination titres were high and hybridomas were stable, so it might be useful in providing a simple, rapid method for the classification and identification of clinical isolates such as pathogenic L. interrogans in place of the complicated and time-consuming conventional methods.
Hua Xi Yi Ke Da Xue Xue Bao 1990 Jun
PMID:[Study on the characteristics of agglutination reaction of McAb with Leptospira interrogans outer envelope]. 239 Oct 93

Two hybridomas which secrete monoclonal antibody (McAb) against polymerized human serum albumin (PHSA) were obtained by the fusion of SP2/0 myeloma cell with immune murine spleen cells. One of the McAb was identified as mouse IgG1, the other was IgM. The titers of these purified McAb was 1:16 364 with passive hemagglutination assay (PHA). After labelling with 125I by chloramine-T method, a solid phase radioimmune assay for detecting the PHSA has yielded in 21 positive results, out of 126 HBsAg positive sera, but 53 HBsAg negative sera were all negative. At present we have not seen any report of PHSA present in circulation. PHSA may be as a bridge bind receptor between HBV and hepatocytes and then initiate infection. The appearance of PHSA in HBsAg positive sera could be the result of the damage of the liver during virus infection. More work should be done for this explanation.
Hua Xi Yi Ke Da Xue Xue Bao 1989 Jun
PMID:[Development and application of hybridoma secreting monoclonal antibody against poly-human serum albumin]. 259 20


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