Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0026764 (multiple myeloma)
 36,148 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Monoclonal antibodies against human prostatic acid phosphatase (PAPase) were produced by immunization of human primary spleen cell cultures. Dissociated spleen cells were cultured for 5-8 days in the presence of 100 ng/ml of PAPase and pokeweed mitogen (1:5000). Following immunization, B cells were isolated and infected with Epstein-Barr virus (EBV). Two weeks after EBV-transformation, cells were fused with either mouse myeloma cells (SP2/OAg14) or human/mouse heteromyeloma cells (SHM-D33). Hybrid clones were screened for anti-PAPase production. In 7 independent immunizations, the average fusion frequency was 3.6 per 10(6) lymphocytes. 18-32% of the hybridomas produced anti-PAPase; approximately 75% of these secreted IgM and 25% secreted IgG. Antibody specificity was determined by immunoassay and immunohistological studies. The procedures described here may be suitable for the production of human monoclonal antibody of a useful specificity.
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PMID:Production of monoclonal antibodies against prostatic acid phosphatase by in vitro immunization of human spleen cells. 241 32

A human hybridoma cell line C8 secreting human monoclonal anti-idiotypic antibodies (alpha Id) was established by fusing Epstein-Barr virus transfected peripheral blood lymphocytes from a hemorrhagic fever with renal syndrome (HFRS) patient with interspecific myeloma SHM-D33 cells. The alpha ID human monoclonal antibody (hMAb) could react with anti-HFRSV Ab1 from different species, and the reaction could be inhibited by HFRSV antigen, indicating that anti-idiotype hMAb bears the internal image of HFRSV antigen. Anti-HFRSV antibodies (Ab3) with neutralizing and hemagglutination inhibition activities in BALB/c mice or rabbits induced by the alpha Id h MAb were produced, suggesting that the internal image beared by anti-idiotype antibodies may be related to neutralizing and hemagglutinating epitopes on HFRSV antigen.
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PMID:Human monoclonal anti-idiotypic antibodies bearing the internal image of hemorrhagic fever with renal syndrome virus antigen. 775 83