Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0026764 (multiple myeloma)
 36,148 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We have determined the in vitro host range of the cloned MO-21 and FL-1 murine myeloma retroviruses grown in SC-1 cells that were originally isolated from cloned MOPC-21 and FLOPC-1 BALB/c plasmacytoma cell lines. These viruses are able to replicate in murine (BALB/3T3, NIH/3T3) as well as numerous heterologous cell lines. These myeloma retroviruses also exhibit mink cell focus-inducing activity. MO-21 and FL-1 shared interference patterns with each other, but their replication was not interfered with by ecotropic, xenotropic, or amphotropic viruses. The lack of cross-interference with ecotropic or xenotropic viruses distinguishes these isolates from other mink cell focus-inducing viruses.
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PMID:BALB/c myeloma retroviruses have mink cell focus-inducing activity. 625 76

A xenoantiserum to murine B16 melanoma cells was developed by immunizing rabbits with cultured B16 melanoma cells. This antiserum could, after extensive absorption with normal C57BL/6 mouse tissues, react with syngeneic (B16) and allogeneic (HP) melanomas as well as with other murine neoplasms, including syngeneic 75S adenocarcinoma, allogeneic myeloma and leukemic T cell lines. The antiserum also cross-reacted with syngeneic fetal fibroblasts and with an allogeneic fetal fibroblast cell line (SC-1) either uninfected or infected with murine leukemia virus (MuLV). Immunoprecipitated material from B16 melanoma cell-surface glycoproteins that had been labeled with [125I] by lactoperoxidase and purified by a Lentil lectin column was analyzed by one-dimensional SDS- and two-dimensional polyacrylamide gel electrophoresis, which disclosed an acidic glycoprotein with a molecular weight (mol. wt) of 90 K daltons. Absorption studies suggested that the 90 K mol. wt gylcoprotein represented the oncofetal moiety expressed in murine medanoma, carcinoma and fetal tissues. When the amount of this antigen in developing C57BL/6 mouse fetuses was measured by absorption assays, we found that it was expressed strongly in those fetuses just before birth. Binding and absorption studies demonstrated that the 90 K mol. wt glycoprotein, while being expressed on a variety of fetal and neoplastic cells in mice, did not exist at detectable levels in normal tissues of adult C57BL/6 mice, including tissues of the thymus, lymph node, spleen, liver, brain, lung and kidney.
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PMID:Identification of an oncofetal antigen (gp90) on murine B16 melanoma cells. 688 31

Four anti-tumor cytotoxic antibodies, namely, A6 of the IgG2 class and B3, C2, and D1 of the IgM class, were obtained in monoclonal form, from hybridization of mouse myeloma cells with spleen cells of normal untreated mice selected for high natural anti-tumor immune responses. The specificity of the four monoclonals was tested by complement-dependent cytotoxicity assay on the reference EL4 lymphoma at different days of in vivo transplant, on normal adult and fetal tissues, on the SC-1 fibroblastic cell line uninfected or infected with a murine ecotropic type-C virus, and on a panel of murine lymphomas of different origin. The four antibodies had different specificities: the A6 and B3 recognized virus-related structures, the C2 a structure expressed on fetal cells, and the D1 a normal component of fibroblasts. The different classes and specificities of the four monoclonals, as well as their in vitro-demonstrated synergistic cooperation, give support to the hypothesis that the natural humoral anti-tumor cytotoxic immune response could be the result of the cooperative activity of an array of heterogeneous antibody molecules.
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PMID:Heterogeneity of the natural humoral anti-tumor immune response in mice as shown by monoclonal antibodies. 689 9