Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0026764 (
multiple myeloma
)
36,148
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Two particular types of sialoglycoproteins have been detected in fish: polysialoglycoproteins containing alpha 2-->8-linked polysialic acid (-->8Neu5Gc alpha 2-->)n present in unfertilized Salmonidae fish eggs, and glycoproteins bearing oligo/polymers of deaminated neuraminic acids (
KDN
) found in the vitelline envelope of the eggs and ovarian fluid. We report the preparation and characterization of a monoclonal antibody specifically recognizing oligo/polymers of
KDN
sequences in glycoproteins and its application in immunohistochemistry. Fusion of spleen cells from a BALB/c mouse immunized with a
KDN
-rich glycoprotein (KDN-gp) containing (-->8KDN alpha 2-->)n-->6(
KDN
alpha 2-->3Gal beta 1-->3G alpha lNA-c alpha 1-->3) GalNAc alpha 1-->residues, with mouse
myeloma
cells yielded a hybrid cell line producing a monoclonal antibody that bound to
KDN
-gp, but not to
KDN
-gp depleted of
KDN
residues. The specificity of the monoclonal antibody, designated mAb.kdn8kdn, was determined by an enzyme-linked immunosorbent assay using
KDN
-gp samples that varied in
KDN
content. These antigens were prepared by the selective removal of
KDN
residues from the native
KDN
-gp. The mAb.kdn8kdn reacted most strongly with the intact
KDN
-gp and less strongly with
KDN
-gp samples containing decreased numbers of
KDN
residues.(ABSTRACT TRUNCATED AT 250 WORDS)
...
PMID:Monoclonal antibody specific for alpha 2-->8-linked oligo deaminated neuraminic acid (KDN) sequences in glycoproteins. Preparation and characterization of a monoclonal antibody and its application in immunohistochemistry. 792 16
