Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0026764 (multiple myeloma)
 36,148 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Seven monoclonal antibodies to a murine mammary carcinoma MM46 were produced by fusing a mouse hybridoma cell line Sp2/O-Ag14 or a myeloma cell line P3-X63-Ag8-U1 with spleen cells from C3H/He mice immunized with MM46. Their specificities were investigated by the complement-dependent cytotoxicity test and binding inhibition assay using 125I-labeled monoclonal antibodies. The complement-dependent cytotoxicity test showed that all of them reacted with MM antigen-positive tumor cells such as MM46 and FM3A/R. Three of them reacted with both C57BL/6 lymph node cells and EL4 tumor cells, suggesting that these 3 antibodies recognize Ly-6.2 antigen. One reacted with all tumor cells so far tested (MM46, FM3A/R, MM48, MH134, and Meth A) except EL4. One antibody that cross-reacted with MH134 was also obtained. Binding inhibition assay confirmed hat the 7 monoclonal antibodies detected at least 4 different epitopes on MM46. These results suggest that there are at least 4 different molecules on the cell surface of MM46: namely, molecules that are 1) restricted to MM antigen-positive tumor cells, 2) present on several tumor cells, 3) broadly distributed on tumor cells, and 4) cross-reactive with Ly antigen.
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PMID:Monoclonal antibodies directed to different tumor-associated antigens on a murine mammary tumor cell line, MM46. 244 8

Four monoclonal antibodies to MH134 murine syngeneic hepatoma cells, 3H1, 7C2, 11G2, and 12A2, were produced by hybridomas constructed by fusing P3-X63-Ag8-U1 murine myeloma cells with spleen cells of a C3H/HeN mouse immunized with the syngeneic tumor cells. Immunodiffusion analysis with rabbit anti-mouse immunoglobulin antisera showed that 3H1, 7C2, 11G2, and 12A2 are IgG2a, IgM, IgG1, and IgG2a, respectively. Enzyme-linked immunosorbent assay using cells of five syngeneic tumor lines, MH134, MM102, MM46, MM48, and X5563, and lymph node cells of C3H/HeN and C57BL/6 mice showed that 3H1 specifically bound to MH134 tumor cells, whereas 7C2, 11G2, and 12A2 reacted not only with MH134 but also with MM102 and MM46 tumor cells. None of these monoclonal antibodies bound either to cells of MM48 or X5563 tumor lines or to normal lymph node cells. These results strongly suggest that MH134 tumor cells display at least two kinds of tumor-associated antigens on their cell surfaces: one is expressed uniquely by MH134 tumor cells, which are recognized by 3H1; the other is commonly shared by MH134, MM102, and MM46 tumor cells, which are determined by the other three antibodies. 3H1, 11G2, and 12A2 but not 7C2 were found to be able to induce antibody-dependent cellular cytotoxicity (ADCC) against MH134 tumor cells. Target specificity of ADCC induced by these monoclonal antibodies was identical with that seen in enzyme-linked immunosorbent assay. 3H1, 7C2, and 12A2 but not 11G2 exhibited complement-dependent cytotoxicity, showing the same specificity in target cell lysis as that seen in enzyme-linked immunosorbent assay or ADCC. Pretreatment of MH134 tumor cells with 7C2 inhibited ADCC of both 11G2 and 12A2. Pretreatment of the tumor cells with 11G2 inhibited complement-dependent cytotoxicity of both 7C2 and 12A2. Neither ADCC nor complement-dependent cytotoxicity of 3H1 was inhibited by the pretreatment of the cells with 7C2 or 11G2. These results strongly suggest that tumor-associated antigens recognized by 3H1 are located apart from that recognized by 7C2, 11G2, and 12A2 and that the binding sites of the latter three antibodies are closely associated with, or identical with, each other in the tumor-associated antigen. The ability of 12A2 to induce ADCC against MH134 tumor cells was significantly stronger than that of 3H1 or 11G2.(ABSTRACT TRUNCATED AT 400 WORDS)
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PMID:Murine tumor cell lysis by antibody-dependent macrophage-mediated cytotoxicity using syngeneic monoclonal antibodies. 397 34

Monoclonal antibodies against MM46, an ascitic mouse mammary tumor of C3H/H2, were produced by fusing mouse myeloma cell line NS-1 with spleen cells from a (BALB/c X C3H/HeN)F1 mice hyperimmunized with MM46, an MM antigen-positive tumor. Eight antibodies showed cytotoxicity against MM46, but not against MM48, an MM antigen-negative ascitic mammary tumor, and one hybridoma produced an agglutinating antibody. One of the cytotoxic monoclonal antibodies, 3-3-C, was selected, and the strain distribution and the tissue distribution of MM antigen were studied. The results demonstrated that MM antigen had a strain distribution identical to Ly-6.2 antigen, and a similar tissue distribution. Therefore, the characterization of MM antigen and Ly-6.2 antigen was investigated. Ly-6.2 antibody was shown to be cytotoxic for MM46, but not for MM48, in accordance with 3-3-C. Genetic segregation analysis of MM and Ly-6.2 antigens in 33 backcross mice demonstrated complete concordance between these two antigens. In addition, MM antigen phenotype of an Ly-6.2 congenic strain, C3H.B6-Ly-6b, was studied, and it was found to be positive in contrast to C3H/HeN. Furthermore, cross-absorption studies revealed that both MM46 cells and C3H.B6-Ly-6b lymph node cells could absorb cytotoxic activities of 3-3-C and monoclonal anti-Ly-6.2 antibody. The results so far obtained suggested strongly that these two loci controlling expression of MM and Ly-6.2 antigens were identical or very closely linked.
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PMID:Production of monoclonal antibodies against MM antigen: the serologic identification of MM antigen with Ly-6.2 alloantigen. 697 71