Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0026764 (multiple myeloma)
 36,148 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

BALB/c mice were immunized with the crude fraction of boar acrosin. Immune spleen cells were fused with myeloma cells SP2/0-Ag14. One of the 6 hybridomas produced was cloned and characterized by ELISA and SDS-PAGE. Possible uses of the monoclonal antibody against acrosin for immunological detection of the amount of acrosin liberated after manipulations with spermatozoa and for selection of undamaged spermatozoa for insemination are discussed.
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PMID:Preparation and characterization of a monoclonal antibody against boar acrosin. 353 Aug 27

Hybridoma cell lines were obtained by fusing NS-1 mouse myeloma cells with splenocytes from female mice immunized with whole washed human spermatozoa. Nine clonally derived cell lines were selected which secrete monoclonal antibodies against integral spermatozoan antigens. Immunofluorescence studies showed specific binding of the individual monoclonal antibodies to localized regions of the sperm cell: acrosomal cap, equatorial segment, and midpiece. Five of these antibodies also recognized antigenic determinants of isolated acrosin. The monoclonal antibodies provide probes for the immunochemical characterization of sperm antigens and for the elucidation of the role of these antigens in spermatozoan function.
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PMID:Analysis of antigen expression on human spermatozoa by means of monoclonal antibodies. 388 Dec 96