Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0026764 (multiple myeloma)
 36,148 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Isoamylases, with an abnormal anodic migration, were detected by an electrophoretic technique in the sera from two patients with immunoglobulin A-type myeloma. The abnormal isoamylase bands migrate towards the anode faster than the salivary isoamylase (S2) band and were stained more strongly than the S2 sub-band. The abnormal isoamylase could be separated from patients' sera by using size-exclusion high-performance liquid chromatography. The serum abnormal isoamylases were showed to be sialic acid residues containing amylase, after the study of treatment with neuraminidase (EC 3.2.1.18), and to be salivary-type amylase, after the study of reaction with human salivary monoclonal antibody. The abnormal bands were not detected in the saliva from one patient. The two patients had no detectable malignancies except myeloma. These findings strongly suggest that the sialic acid-containing salivary-type amylases were produced ectopically from myeloma cells. In this regard the ectopic amylase production by myeloma cells is discussed.
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PMID:Separation and characterization of sialic acid-containing salivary-type amylase from patients' sera with immunoglobulin A-type myeloma. 172 78

The phenotype of tumor-producing amylase is usually salivary-type amylase, in contrast, we experienced sialyl salivary-type amylase detected in the sera of the patients with malignancies. Sialyl salivary-type amylase, with an abnormal anodic migration, was detected by isoamylase electrophoretic analysis in the sera of patients with multiple myeloma and with ovarian cancer. The abnormal isoamylase bands migrated toward the anode faster than the salivary isoamylase band(S2) and were stained more strongly than S2 band. The isoamylases could be separated from residual normal isoamylases in the sera of patients by using gel permeation chromatography. The isoamylases were showed sensitive by neuraminidase treatment and were reacted with anti-human salivary monoclonal antibody.
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PMID:[Tumor producing amylase]. 1216 74