Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0026764 (
multiple myeloma
)
36,148
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A
myeloma
cell line (KHM-4) from a patient with
multiple myeloma
and idiopathic hyperammonemia, and another
myeloma
cell line (RPMI8226) were seen to have activity to form ammonia from arginine. High activity of
ornithine transcarbamylase
(
OTC
), a hepatic urea cycle enzyme, was detected in these cell lines.
OTC
of these cells was much more heat-stable than the liver enzyme, and did not cross-react with an antibody against the liver enzyme. As the
OTC
activity was also detected in the culture medium of the
myeloma
cells and because the activity was markedly decreased by the antimycoplasma drug MC-210, the
OTC
activity was assumed to be associated with mycoplasma infection. Polymerase chain reaction, using degenerate oligonucleotide mixtures corresponding to the two highly conserved sequences of
OTC
, amplified a DNA sequence that apparently encodes a portion (about 67% in length) of mycoplasma
OTC
. The predicted amino acid sequence of the mycoplasma enzyme was 33-47% identical with those of the enzymes of bacteria, yeast and mammals.
...
PMID:A novel ornithine transcarbamylase present in mycoplasma-infected myeloma cells. 819 71
A monoclonal antibody (mAb) specific to ochratoxin A (OTA) was produced from a stable hybridoma cell line, 9C9H9, generated by the fusion of P3/NS1/1-AG4-1
myeloma
cells with spleen cells isolated from a BALB/c mouse immunized with OTA-keyhole limpet hemocyanin. The 9C9H9 mAb belongs to the immunoglobulin G1 (kappa chain) isotype. A competitive direct enzyme-linked immunosorbent assay (cdELISA) and a competitive indirect ELISA were established for antibody characterization. The concentrations causing 50% inhibition of binding of OTA-horseradish peroxidase to the antibody by OTA, OTB, and
OTC
were found to be 0.32, 0.17, and 0.28 ng/mL, respectively, in the cdELISA. A sensitive and rapid mAb-based gold nanoparticle immunochromatographic strip was also developed using this mAb. This strip has a detection limit of 5 ng/mL for OTA and can be completed in 10 min. Analysis of OTA in coffee samples revealed that data obtained from immunochromatographic strip were in a good agreement with those obtained from cdELISA. The mAb-based cdELISA and immunochromatographic strip assay established in this study were sensitive and accurate for rapid screening of OTA in coffee samples.
...
PMID:Development of a monoclonal antibody against ochratoxin A and its application in enzyme-linked immunosorbent assay and gold nanoparticle immunochromatographic strip. 1869 2
