Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0026764 (multiple myeloma)
 36,148 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Four hybridomas stably secreting monoclonal antibodies (McAbs) against human growth hormone (hGH) have been obtained by fusion of immunized mice spleen cells with myeloma SP2/0 cells. After inoculation of hybridoma cells to BALB/c mice i.p., the ascitic fluids were collected and characterized, the titre of the 4 McAbs being in the range of 0.6-10.0 X 10(5). As determined by Ouchterlony analysis, all the 4 McAbs were IgG1. They varied in affinities, with equilibrium constants from 0.53 X 10(9) to 9.0 X 10(9) L/mol. McAb-1 showed 52.5% cross-reactivity with human placental lactogen (hPL). McAb-2, McAb-3 and McAb-4 displayed no crossreactivity with hPL and human prolactin (hPRL). Antigenic determinants recognized by the 4 McAbs were mapped through their reactions with hGH fragment consisting of residues 1-43, cross-reaction with hPL, hPRL and antibody-antibody competition test. The results showed that the antigenic determinants of the McAbs are not located in the site of hGH comprising residues 1-43 but on the three non-overlapping antigenic sites of hGH. As shown in receptor assay, all the 4 McAbs exhibited specific inhibition on the binding of pregnant rabbit liver GH receptor to 125I-hGH. It seems likely that receptor-binding site is larger than the antigenic determinant or there are more than one site on hGH surface capable of binding to the hormone receptor. The McAbs could only bind with the peak 2 (MW22000) but not the peak 1 (MW 45000) of old 125I-hGH after gel filtration.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:[Preparation and characteristic analysis of monoclonal antibodies against human growth hormone]. 247 70

A chimeric human-mouse anti-T lymphocyte mAb (CHT2; SDZ 214-380) has been constructed by cloning the variable region exons of both the L and H chains from the murine hybridoma RFT2 which have CD7 specificity and reactivity with a 40-kDa Ag. The variable L chain exon was joined to the human C kappa, and the variable H chain exon was joined to the human IgG1 region exon encoding the human allotype nGlm(z), nGlm(a). The gene constructs were introduced by electroporation into SP2/0, a non-Ig-producing murine myeloma. The identical tissue reactivity of the newly made CHT2 and the original murine RFT2 mAb (CD7) was confirmed by blocking experiments as well as by immunohistology and flow cytometry. Because this new mAb may have clinical use, the CD7 Ag expression of T lineage cells has also been quantitated in double and triple immunofluorescence assays in combinations with mAb to restricted forms of leukocyte common Ag that designate unprimed (CD45R+) and primed T lymphocyte populations (UCHL1+). CHT2 shows very strong reactivity with large thymic blast cells and cortical thymocytes from which T-ALL originates. Strong staining is seen on CD45R+ unprimed "virgin" T lymphocytes, whereas the expression on UCHL1+ primed "memory" cell types is weaker unless these cells are reactivated by mitogens or Ag. Thus CHT2 may spare a substantial population of resting memory T cells which is relevant to its potential therapeutic use. In addition the chimeric antibody had a greater in vitro antibody dependent cytotoxicity and a prolonged half-life (4.2 to 5.0 days) in Rhesus monkeys.
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PMID:Characterization of a human T cell-specific chimeric antibody (CD7) with human constant and mouse variable regions. 247 83

The HindII fragment of human interferon beta (IFN-beta) gene was inserted downstream from SV40 late promoter in pSV2-dhfr and cotransfered with pSV2-gpt into the mouse myeloma SP2/0 cells which were hypoxanthine guanine phosphoribosyl transferase (HGPRT) deficient. After the selection in HAT (Hypoxanthine Aminopterine Thymidine) medium containing myciphenolic acid and xanthine, the efficient constitutive expression of IFN-beta could be detected in the supernatant of the survive cells.
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PMID:[Expression of human interferon beta gene controlled by SV40 late promoter in mouse myeloma SP2/0 cells]. 248 40

Primary infection with Coccidioides immitis is commonly accompanied by the production of an immunoglobulin M precipitin antibody which is detected by the tube precipitin (TP) assay or by the immunodiffusion assay for TP antibody (IDTP assay). In the present investigation, spleen cells from spherulin-immunized BALB/c mice were fused with SP2/O Ag14 myeloma cells, and the resulting hybridomas were screened for antibody to the IDTP antigen by using an enzyme-linked immunosorbent assay. Positive hybridomas were cloned by limiting dilution and injected into pristane-primed mice for ascites production. Characterization of antibody reactivity was accomplished with the IDTP assay, two-dimensional immunoelectrophoresis, and immunoblotting. An immunoglobulin G1 monoclonal antibody which reacts with the IDTP antigen of C. immitis is described. The epitope that is recognized by the monoclonal antibody is also present, but to a lesser extent, on a second coccidioidal antigen which has been designated antigen 2. The monoclonal antibody was not reactive in immunoblots of histoplasmin or blastomycin, indicating that the epitope recognized by this antibody may be specific for C. immitis.
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PMID:Development and characterization of a monoclonal antibody against the tube precipitin antigen of Coccidioides immitis. 249 8

Hybridoma cell lines were produced by fusion of SP2/0 murine myeloma cell line with the spleen cells of Wister rats which were immunized with IgG2a-binding protein isolated from the detergent lysate of a murine macrophagelike cell line, P388D1, by affinity chromatography on IgG-Sepharose 4B. A monoclonal clone (designated as 3A2) out of a total of 13 different antibody-secreting cell lines was found to secrete IgG1 class antibodies, which inhibited more than 70% of the binding of radio-iodinated myeloma IgG2a protein to P388D1 cells. The 3A2 Fab fragments bound specifically to P388D1 cells at 4 degrees C with a KD of 1.9 x 10(-8) M and Bmax of 2.9 x 10(5) per cell. This Fab fragment also specifically bound to Fc gamma 2a receptor (R)-positive T cell line (S49) with a KD of 4.4 x 10(-9) M and a Bmax of 1.0 x 10(4) but did not bind to Fc gamma 2a-negative S49 variant cell line, cyc-. The flow cytometric analysis with the use of fluorescein-isothiocyanate-tagged 3A2 F(ab')2 also showed that this antibody binds to Fc gamma 2aR-positive cells, P388D1 and S49, but not to Fc gamma 2aR-negative cells, cyc-. Monomeric and heat-aggregated IgG2a (13-fold molar excess) inhibited the binding of the radioiodinated 3A2 F(ab')2 to P388D1 cells by 70 and 49%, respectively, whereas the inhibition by monomeric and heat-aggregated IgG2b was 17 and 39%, respectively; 3A2 F(ab')2 (100-fold molar excess) inhibited the binding of IgG2a and IgG2b to P388D1 cells by 90 and 24%, respectively, whereas the inhibition of binding of these IgG to S49 cells was 79 and 49%, respectively. Western blotting analysis showed that 3A2 antibody recognizes a major protein (Mr = 100,000) and a minor component (Mr = 80,000) separated by SDS-PAGE of P388D1 or S49 cell lysates under nonreducing condition, whereas under reducing condition, this antibody recognized a major protein (Mr = 50,000) and two additional minor components (Mr = 40,000 and 35,000). Fc gamma 2aR may thus exist at the cell surface as a disulfide linked dimer of a subunit of Mr of 50,000, which could be partially degraded during the isolation to smaller fragments of 40,000 and 35,000 Mr peptides which are still held together by interchain disulfide bond.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Production and characterization of monoclonal antibodies to Fc gamma 2a-binding protein isolated from the detergent lysate of a murine macrophagelike cell line, P388D1. 252 80

Thirty-five hybridoma cell lines secreting monoclonal antibodies (Mabs) against bovid herpesvirus-4 (BHV-4) strain V. Test were produced. These hybrid cells resulted from the fusion of SP2/0 myeloma cells with splenocytes of BALB/c mice previously immunized with purified BHV-4. A modified indirect fluorescent antibody test (IFAT) was applied as a screening procedure and was compared with an indirect enzyme-linked immunosorbent assay. The selected Mabs were tested by the same IFAT against a panel of BHV-4 field isolates and against bovine herpesvirus-1, bovine herpesvirus-2 and alcelaphine herpesvirus-1 (AHV-1). Comparison of BHV-4 field isolates with Mabs confirmed their close antigenic relationships, but slight antigenic differences were observed between different isolates. One of the Mabs also reacted against AHV-1, indicating an antigenic relationship between BHV-4 and AHV-1. None of the Mabs reacting with BHV-4 possessed neutralizing activity against the strain used for immunization.
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PMID:Production and characterization of monoclonal antibodies to bovid herpesvirus-4. 254 20

Spleen cells from Balb/c mice immunized in sequence with five human gastric cancer cell lines were fused with murine myeloma cell line SP2/0. Hybridomas 3F4, 3G9 and 3H11 secreting monoclonal antibodies (mAb) against gastric cancer were obtained through selective culture and screening. These mAb produced by the immunization procedure have good selectivity and high positive rate in reaction on gastric cancer. The positive rate of reaction on gastric cancer cells and tissues could reach 5/5 and 84.8-93.5%, respectively, whereas there was almost no positive reaction on normal cells and tissues As there was no correlation between the positive reaction of gastric cancer and their histopathologic typing, and the cross reaction of mAb with other tumors and fetal gastrointestinal tissues was quite high, the corresponding antigens of these mAb were considered as extensive oncofetal antigens.
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PMID:[Monoclonal antibodies against gastric cancer and their selective reaction on various tissues]. 255 69

Like other small-sized neurotransmitter molecules, glutamate (Glu) was conjugated to carrier proteins via glutaraldehyde (G). Human serum albumin (HSA) and thyroglobulin (TH) conjugates were alternately injected into mice. When a relevant immune response was obtained for antibody affinity and specificity, hybridization of spleen activated lymphocytes with SP2/O/Ag myeloma cells was performed. Supernatant culture media of hybridomas were tested for the presence of anti-conjugated Glu antibodies with our ELISA method. Selected hybridomas giving good antibody affinity and specificity were then cloned by the limiting dilution technique. Using DEAE-chromatographed ascites fluid, Glu reactivity was observed on the cortex and the hippocampus. Staining obtained with this monoclonal antibody was in agreement with that observed with previous polyclonal antisera directed against conjugated Glu or monoclonal anti-gamma-glutamyl-Glu antibody.
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PMID:Monoclonal antibody directed against glutaraldehyde conjugated glutamate and immunocytochemical applications in the rat brain. 256 32

Various immunizing regimens, cell culture requirements and cell fusion conditions were examined for efficient production of hybridomas secreting anti-foot-and-mouth disease virus (FMDV) antibodies. A highly sensitive streptavidin-biotin-based enzyme-linked immunosorbent assay (ELISA) was used for screening of hybridomas for specific antibody production as well as for determining the serotype specificity of the antibodies. Six hybridoma cell lines generating antibodies to FMDV type Asia-1 (vaccine strain 63/72) were produced by fusion of SP2/0 mouse myeloma cells and spleen cells from mice immunized with the inactivated viral antigen. The monoclonal antibodies (MoAb) from four producer clones reacted in ELISA specifically with the intact virus antigen of type Asia-1 without any cross-reactivity with strains of other virus types 0, A and C. Two other clones positive for anti-Asia-1 virus antibodies in ELISA cross-reacted with type C virus strain. The MoAb from three of the four Asia-1 virus type specific clones neutralized the infectivity of the immunizing viral strain. One neutralizing MoAb reacted with the separated VP1 from the immunizing viral strain in immunoblotting.
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PMID:Hybridoma cell lines secreting monoclonal antibodies to foot-and-mouth disease virus type Asia-1. 256 7

A hybridoma secreting a monoclonal antibody (mAb) with specificity for tumor-associated cell surface antigens of a transplantable murine mammary adenocarcinoma (SMC-168) was prepared by fusion of syngeneic C3H/He spleen cells with SP2 myeloma cells. Mice which were pretreated with this mAb (C-73) were significantly resistant to the outgrowth of a tumorigenic dose of SMC-168 cells when compared to controls. The treated mice developed tumor-specific cell-mediated immunity, measured by leukocyte adherence inhibition (LAI), which was equal to that of mice immunized with live tumor cells. The IgG fraction from serum of mice receiving mAb C-73 contained antibodies which would bind to that mAb suggesting the presence of anti-idiotypic antibodies (anti-Id). This binding could be partially inhibited by a soluble 1-butanol cell surface extract of SMC-168. Rabbits were immunized with mAb C-73 to produce a polyclonal anti-Id. The purified and absorbed IgG fraction of this serum would bind only to mAb C-73 and not to other mAbs of the same isotype or normal C3H/HeN IgG. Binding of the rabbit anti-Id to mAb C-73 could be partially inhibited by soluble tumor-associated antigen extracted from SMC-168. Mice immunized with this polyclonal anti-Id vaccine developed tumor-specific cell-mediated immunity and were significantly resistant to the outgrowth of a tumorigenic dose of SMC-168.
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PMID:Protection against a syngeneic mammary adenocarcinoma by administration of idiotypic and anti-idiotypic antibodies. 258 49


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