Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0026764 (multiple myeloma)
 36,148 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A total of 204 sera, taken from healthy individuals or from individuals with various parasitic and bacterial infections, were examined by the indirect haemagglutination test. The tests were carried out using either a thermo-stable lipoprotein or unfractionated hydatid cyst fluid, and a titre of 1:64 or above was considered positive. Sixty-two of 70 sera from individuals with surgically-confirmed hydatid disease showed positive reactions with the thermo-stable lipoprotein--a sensitivity of 88%. No false positive reactions were obtained with sera from healthy individuals or from individuals with parasitic or bacterial infections, and no cross-reactions were observed with sera from individuals with multiple myeloma. The lipoprotein antigen thus showed a specificity of 100%. A sensitivity of 88% was obtained with the indirect haemagglutination test using whole hydatid cyst fluid; but positive reactions were obtained from healthy individuals and from individuals with schistosomiasis, leishmaniasis, taeniasis and malaria. No cross-reactions were obtained with sera from patients with gonorrhoea, syphilis or multiple myeloma. Because of the high sensitivity and specificity shown by the thermo-stable lipoprotein ('Antigen 880'), it is considered that this antigen is more useful than unfractionated hydatid cyst fluid in the diagnosis of human hydatidosis in Kenya.
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PMID:Diagnosis of human hydatid disease in surgically-confirmed cases by the use of the indirect haemagglutination test based on a thermo-stable lipoprotein and on unfractionated hydatid cyst fluid. 260 68

This communication reports on the usefulness of the IHA test and the ELISA in the diagnosis of human hydatid disease. The study was conducted on 40 surgically confirmed cases of hydatid disease, 40 normal individuals, and sera from individuals with various parasitic infections and other conditions namely: hook-worm-8, taeniasis-5, schistosomiasis-10, malaria-15, visceral leishmaniasis-12, multiple myeloma-3, syphilis-6, and gonorrhoea-10. The results show a sensitivity of 85% and specificity of 100%. The results indicate that it is no longer scientifically rational to hold the view that the Turkana do not mount adequate immune response against Echinococcus infections.
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PMID:Usefulness of indirect haemagglutination (IHA) and enzyme-linked immunosorbent assay (ELISA) in the diagnosis of human hydatidosis. 267 69

Hybridomas secreting monoclonal antibodies (MAbs) to Trichinella spiralis were produced. Myeloma cells were fused with splenocytes of a mouse immunized with excretory-secretory (E-S) antigen of infective larvae. A large percentage of growing hybrids secreted antibodies cross-reactive to many of 23 heterologous parasites tested. Only 6 monoclones (designated 3F2, 5D1, 10F6, 11E4, 13D6 and 14D11) secreted MAbs specific to the E-S antigen and/or a crude extract (CE) of T. spiralis infective larvae. The 6 monoclones secreted IgM, IgG3, IgM, IgG3, IgG3 and IgG3, respectively. Clone 5D1 was selected to mass produce MAbs which were then coupled to CNBr-activated Sepharose CL-4B to prepare an affinity-purified antigen. Dot-blot ELISA with either purified antigen or CE was evaluated. There were 17 patients with acute trichinellosis and 76 individuals convalescing from T. spiralis infection (group 1). Controls were 170 patients with parasitic infections other than trichinellosis (group 2) and 35 healthy parasite-free controls (group 3). CE-ELISA was positive in all group 1 patients. However, sera from many group 2 patients also were reactive (opisthorchiasis-44.2%, schistosomiasis-44%, gnathostomiasis-30%, paragonimiasis-28.6%, taeniasis-27.3%, strongyloidiasis-23.1% and hookworm infections-20%). Affinity-purified antigen was 100% specific, all sera from group 2 and group 3 individuals tested negative. Although 74 of 76 patients (97.4%) with convalescing trichinellosis tested positive, sera from only 3 of 17 patients (17.6%) with acute T. spiralis were reactive. Thus, CE antigen is appropriate when sensitivity is needed, while purified antigen should be used when specificity is required. Dot-blot ELISA is easier to perform, more rapid and less expensive than indirect ELISA. Many samples can be assayed simultaneously, special equipment is not required, and results can be preserved for retrospective analysis. Dot-blot ELISA is therefore the method of choice for the rapid diagnosis of trichinellosis, particularly when more complex laboratory tests are unavailable.
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PMID:Trichinella spiralis-specific monoclonal antibodies and affinity-purified antigen-based diagnosis. 1254 56