Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0026764 (multiple myeloma)
 36,148 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A family of monoclonal antibodies (MoAb) was derived from the somatic cell fusion of P3NS1 myeloma cells and lymphoid cells from naturally infected mice of hyperimmunized mice. C57BL/6 mice were naturally infected with Schistosoma japonicum, and BALB/c mice were hyperimmunized with preparations of S. japonicum soluble egg antigens (SEA). The MoAbs which reflected the immune repertoire of naturally infected animals versus hyperimmune animals were characterized with regard to antibody isotype, antigen binding specificity, in-vitro immunosuppression of antigen-induced cell-mediated immune responses and the expression of SJ-CRIM, a major cross-reactive idiotype which appears on polyclonal anti-SEA antibodies generated during murine S. japonicum infection. The data indicate that for MoAbs of the IgG isotype which bound SEA by ELISA, the most immunosuppressive anti-SEA MoAbs identified expressed SJ-CRIM and were derived from naturally infected mice. All anti-SEA MoAbs expressing SJ-CRIM showed an identical banding pattern on immunoblot analysis which was abrogated by weak periodate treatment. The generation of expression of SJ-CRIM on MoAbs using differing methodologies across an allotype barrier indicates that the expression of SJ-CRIM is encoded by a germline gene. These data indicate an association between expression of this germline interstrain cross-reactive idiotype and immunosuppressive capacity. In addition, the immunoregulatory network which develops during immune S. japonicum infection is initiated by a carbohydrate epitope(s) found on various SEA. These data have profound implications in the use of the cross-reactive idiotype as a serodiagnostic tool in schistosomiasis.
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PMID:Characterization of a family of monoclonal antibodies which bind Schistosoma japonicum egg antigens and express an interstrain cross-reactive idiotype. 169 Aug 72

In the present paper, the results of detecting circulating antigen and/or antigen-antibody complexes by McAb against surface membrane antigen of adult Schistosoma japonicum were reported. The McAb, coded as 8SE4, was prepared by fusion of SP2/0 myeloma cells with spleen cells of the BALB/c mice immunized with the saline extract of adult S. japonicum. The 8SE4- directed antigen was proved to be located on the surface of the adult worm. After being purified by a DE52 column, 8SE4 was labelled with HRP and the conjugate (HRP-8SE4) was used in the test. For testing, the serum sample was first incubated with HRP-8SE4, then PEG (mw. 6,000) was added to precipitate the antigen-antibody complex. Upon centrifugation, OPD was added to the precipitate. Results were read by ELISA reader at 492nm. The OD value was found to be proportional to the amount of circulating antigen and/or antigen-antibody complexes. Results from 5 heavily infected (1,500-2,000 cercariae) rabbits showed that the OD values were raised significantly at the 6th week post infection, being 1.9-4.5 times higher than those before infection. The OD values of the 5 rabbits each lightly infected with 10-500 cercariae were also markedly raised 6 weeks post infection and reached the peak at the 8th week, then maintained in high levels until 11th week post infection. The worm burden of the 5 lightly infected rabbits were 4-326. No obvious correlations between OD values and worm loads were observed. The results suggested the existence of surface membrane-related antigen and/or antigen-antibody complexes in the circulation of infected rabbits.
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PMID:[Detection of circulating antigen and/or antigen-antibody complex by using McAb against surface membrane antigen of adult Schistosoma japonicum]. 209 94

Monoclonal antibodies were produced by the fusion of splenic lymphocytes from BALB/c mice immunized with Schistosoma japonicum excretory/secretory antigen and the myeloma cell line SP2/0. The 1B2E7B8 McAb was proved to be specific against the gut antigen of adult worm in IFA. The McAb labelled with HRP was used in Dot-ELISA to detect schistosome circulating antigen. Schistosome circulating antigen was detected in 152 out of 188 proven cases of schistosomiasis, accounting for a positive rate of 80.9%. The positive rates for circulating antigen in cases with 1-24, 25-99 and greater than or equal to 100 EPG were 76.8%, 86.6% and 100% respectively. 10 out of 11 cases who had been checked 2 months after effective treatment became ELISA negative. No circulating antigen was detected in cases with other parasitosis nor in normal individuals. In addition, the McAb-Dot-ELISA showed good reproducibility. The results indicated that McAb-Dot-ELISA might be used for diagnosis of schistosomiasis and evaluation of cure.
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PMID:[Dot-ELISA using monoclonal antibody for detecting schistosome circulating antigen]. 212 59

Fusions between spleen cells from BALB/c mice infected with Schistosoma japonicum or mansoni cercariae and SP2/0 myeloma were carried out. More than 30 hybridoma cell lines secreting monoclonal antibodies were obtained. Their target antigens were integument, gut and egg respectively. The preliminary tests showed that the antibody against gut-associated polysaccharide antigen had the highest activity of all antibodies obtained. This antibody can be used in the sandwich ELISA to detect trichloroacetic acid soluble antigen at a level of less than 1 ng/ml, A positive reaction was found in a group of infected rabbits, negative in normal and the circulating antigen level correlated with the number of infecting cercariae. Three months after treatment antigen titers of 6/8 rabbits became negative. This report shows that the McAb sandwich ELISA for the detection of circulating antigen is better than other antibody detecting methods. It is also a potential immunodiagnostic method.
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PMID:[McAb-ELISA for detection of circulating antigen from schistosoma. I. Preparation, selection of monoclonal antibody and preliminary testing]. 215 Mar 53

A monoclonal IgE antibody was prepared by fusion of NS-1 myeloma cells with spleen cells of C3H/He mice immunized with an extract of adult worms of Schistosoma japonicum (Sj). The antibody was able to elicit passive cutaneous anaphylaxis in the rat skin against Sj with the highest titer of 1/256,000 in an ascitic form but did not cross-react with any of antigens extracted from S. mansoni, Fasciola hepatica, Paragoniumus westermani, or Trichinella spiralis. Western blot analysis indicated that the monoclonal IgE antibody recognized epitopes on molecules of 82 kDa, 97 kDa, 160 kDa, and 200 kDa, at least some of which were recognized by IgG antibodies of patients with chronic schistosomiasis japonica. The IgE antibody also recognized a 97-kDa antigen expressed on the surface of mechanically transformed schistosomula. Passive transfer of the antibody into mice in an early stage of challenge infection resulted in a partial but significant reduction of recovery of adult worms. However, similar treatment was not effective for the protection if the antibody was given in the postlung stage of the infection. Moreover, eosinophil-mediated damage to schistosomula was observed in vitro in the presence of the monoclonal anti-Sj IgE antibody, whereas the damage was not observed in the presence of another monoclonal IgE antibody with dinitrophenyl specificity.
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PMID:Production and properties of a mouse monoclonal IgE antibody to Schistosoma japonicum. 311 84