Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0026764 (multiple myeloma)
 36,148 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Somatic cell hybrids (hybridomas) between mouse myeloma cells and spleen cells derived from BALB/c mice immunized with inactivated rabies vaccine were found to produce antibodies to rabies virus. Monoclonal antibodies with different specificities were obtained either from the mass culture directly after fusion or from clones derived from a single-cell cloning procedure. Several strains of fixed or street rabies virus were analyzed by virus neutralization procedures which demonstrated differences in their antigenic composition. Hybridoma antibodies were able to protect experimental animals from lethal rabies virus infection.
 ...
PMID:Monoclonal antibodies against rabies virus produced by somatic cell hybridization: detection of antigenic variants. 27 9

Production of monoclonal antibodies against a wild strain of rabies virus. Cell fusion of SP 2/O, a murine myeloma against a wild strain of rabies virus has originated five monoclonal antibodies (M.A.) specific for virus nucleocapsid , one M.A. specific for virus glycoprotein and one M.A. specific for a viral membrane protein.
 ...
PMID:[Production of monoclonal antibodies against a wild strain of rabies virus]. 130 44

A series of hybridomas secreting monoclonal antibodies (MCA) to glycoprotein and nucleocapsid proteins of rabies virus strain Vnukovo-32 was selected as a result of fusion of splenocytes from immune BALB/c mice with cells of myeloma line Sp2/OAq14, screening and cloning by limiting dilution methods in semi-liquid agar. Four hybridomas secreted MCA to glycoprotein in high titres (5.0 x 10(5)-2.2 x 10(6)) and had marked virus-neutralizing and therapeutic properties. Eight hybridomas produced MCA to the nucleocapsid complex: five hybridomas secreted MCA of the G class in high titres (2.4 x 10(5)-1.6 x 10(6)) and three hybridomas secreted MCA of the M class in low titres.
 ...
PMID:[The isolation and characteristics of hybridomas producing monoclonal antibodies to the structural proteins of the Vnukovo-32 strain of the rabies virus]. 179 88

According to a recommendation from WHO (World Health Organisation) for prevention of a possible rabies infection, active vaccination has to be combined with application of immunoglobulin to get a fast protective effect. At present, preparations of purified human or equine rabies-specific immunoglobulin are used. We have generated a human rabies-specific monoclonal antibody (huMAb) by immortalization of human B-cells with Epstein Barr Virus (EBV), followed by fusion with a mouse myeloma cell. The resulting clone TW-1 secrets an IgG1 lambda huMAb which specifically reacts in ELISA with 5 laboratory rabies virus strains of serotype 1 and DUV3 (Duvenhage, serotype 4). Western Blot analysis revealed fine specificity for the G glycoprotein (gp67) of rabies virus. HuMAb TW-1 neutralizes rabies virus in vitro (RFFIT) as well as in vivo and protects rabies infected mice. Compared to polyclonal human rabies immunoglobulins, huMAb TW-1 is advantageous, because of its defined specificity and the very low amounts of total protein needed for therapeutic effects.
 ...
PMID:A rabies-specific human monoclonal antibody that protects mice against lethal rabies. 180 70

Hybridomas were prepared by fusion of spleen cells from BALB/c mice immunized with dog rabies isolates from Nigeria with P3x63Ag8 myeloma cells. More than 69 hybridomas secreted antinucleocapsid (antiNC) antibodies when tested with homologous viruses by indirect immunofluorescence. One hybridoma (Z144-88) was found which secreted antiNC antibody that reacted negatively with fox rabies isolates from the Federal Republic of Germany, Switzerland and France and with rabies-related viruses and European bat isolates. It reacted positively with other strains/isolates of rabies virus. It is possible to use this antiNC monoclonal antibody (mab) for the investigation of fox rabies outbreaks in Europe.
 ...
PMID:Identification of fox rabies by a monoclonal antibody directed against nucleocapsid of a street rabies virus. 236 25

Spleen lymphocytes from donor mice immunized against rabies virus were transferred into non immunised cyclophosphamide treated syngeneic mice. Antibody producing cells against rabies antigens were increased in the cyclosphosphamide treated recipient mice (p less than 0.01) as compared to then non treated recipient mice. A fusion was made with the splenic cells of cyclophosphamide treated recipient mice and SP2 murine myeloma cells 30 of 144 hypridoma plated welles were positive. There results suggest that cyclophosphamide immunosuppression inhibits the regulation of antibody production.
 ...
PMID:[Increase of lymphocytes producing antibodies against rabies virus, using intrasplenic adoptive transfer in mice treated with cyclophosphamide]. 263 21

Twenty-one hybridoma cultures, obtained through the fusion of mouse myeloma cells with splenocytes of BALB/c mice immunized with either rabies virus or Mokola virus, secreted monoclonal antibodies specific for the nucleocapsid of the inducer virus. They displayed different specificities for the nucleocapsids of rabies and rabies-related viruses and could be classified into eight groups which are likely to correspond to different antigenic determinants on the nucleocapsid. Four strains of fixed rabies virus (CVS, ERA, Flury-LEP and Kelev) could not be differentiated by the nucleocapsid-specific hybridoma antibody. The Flury-HEP virus (derived from Flury-LEP) as well as the rabies-related viruses Mokola, Lagos bat and Duvenhage, showed marked differences in their reactivities with hybridoma antibodies to nucleocapsid. A selected panel of three of these hybridomas may be used for a rapid differential diagnosis among all members of the Lyssavirus group.
 ...
PMID:Use of hybridoma monoclonal antibodies in the detection of antigenic differences between rabies and rabies-related virus proteins. I. The nucleocapsid protein. 615 36

A rapid and sensitive enzyme immunoassay is described for detecting rabies antibody in hybridoma culture fluids. Glass fiber filter disks were used to immobilize gamma-irradiated mouse neuroblastoma cells infected with street or laboratory strains of rabies virus. Bound rabies-specific antibody was detected by reaction with horseradish peroxidase-labeled goat anti-mouse immunoglobulin G. The assay was performed in a 96-well filtration device developed by Cleveland et al. (J. Clin. Microbiol. 15:402-407, 1982) for the typing of herpes simplex viruses. When partially disrupted cells were used, both internal and external viral antigens were available for reaction. The procedure is rapid (less than 4 h for completion) and requires only small amounts of fluid, and the gamma-irradiated antigen is noninfectious. When the procedure was used to screen 145 fluids from rabies-immune spleen-myeloma cell fusions, 132 were positive for rabies antibody. Other commonly used assays for the detection of rabies-specific antibody were less sensitive. Simultaneous analyses of many hybridoma fluids against a battery of street and laboratory strains of rabies virus are possible and allow rapid selection of useful monoclones.
 ...
PMID:Enzyme immunoassay for rabies antibody in hybridoma culture fluids and its application to differentiation of street and laboratory strains of rabies virus. 636 63

Assuming the fact that the highest specific secreting hybrids number has been recorded with Iq secreting spleen cells, we did some immunizing protocols to maximize the ELISA reacting antibodies: for this Balb/c mice were primed intra-peritoneally with vaccine containing inactivated rabies (P.V. strain). Half of them were boosted intravenously with the same concentrated purified and live virus. We performed a series of hybridizations, at different times (i.e. 10, 15, 20, 75 days), between the rabies committed splenocytes and the SP 2/0 non secreting myeloma cells. Successful hybrids secreting antibody to rabies virus have only been obtained at the earliest and the latest time of fusion, essentially with the intravenous booster. They were initially screened by ELISA test for PV binding and about 65% of the colonies were generally positive. Clones directed against nucleocapsids were revealed by immunofluorescence of the rabies infected fixed cells, and those directed against glycoprotein by the neutralizing test and rabies infected live cells. From the early fusion we also produced hybrids secreting IgG (60%) or IgM (40%). At that time IgG/IgM distribution of specific hybrids do not correlate to that seen for ELISA reacting antibodies. It suggests that the IgG secreting spleen cells are the preferential fusion partners or the most vigorous ones.
 ...
PMID:Production of monoclonal antibodies against the Pasteur (P.V.) strain of rabies virus: problems and results. 652 39

We have developed idiotype-anti-idiotype monoclonal antibodies that provide evidence for rabies virus binding to the acetylcholine receptor (AChR). Hybridoma cell lines 7.12 and 7.25 resulted after fusion of NS-1 myeloma cells with spleen cells from a BALB/c mouse immunized with rabies virus strain CVS. Antibody 7.12 reacted with viral glycoprotein and neutralized virus infectivity in vivo. It also neutralized infectivity in vitro when PC12 cells, which express neuronal AChR, but not CER cells or neuroblastoma cells (clone N18), which have no AChR, were used. Antibody 7.25 reacted with nucleocapsid protein. Anti-idiotypic monoclonal antibody B9 was produced from fusion of NS-1 cells with spleen cells from a mouse immunized with 7.12 Fab. In an enzyme-linked immunosorbent assay and immunoprecipitation, B9 reacted with 7.12, polyclonal rabies virus immune dog serum, and purified AChR. The binding of B9 to 7.12 and immune dog serum was inhibited by AChR. B9 also inhibited the binding of 7.12 to rabies virus both in vitro and in vivo. Indirect immunofluorescence revealed that B9 reacted at neuromuscular junctions of mouse tissue. B9 also reacted in indirect immunofluorescence with distinct neurons in mouse and monkey brain tissue as well as with PC12 cells. B9 staining of neuronal elements in brain tissue of rabies virus-infected mice was greatly reduced. Rabies virus inhibited the binding of B9 to PC12 cells. Mice immunized with B9 developed low-titer rabies virus-neutralizing antibody. These mice were protected from lethal intramuscular rabies virus challenge. In contrast, anti-idiotypic antibody raised against nucleocapsid antibody 7.25 did not react with AChR.
 ...
PMID:Evidence from the anti-idiotypic network that the acetylcholine receptor is a rabies virus receptor. 767 60


1 2 Next >>