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Query: UMLS:C0024623 (gastric cancer)
 36,219 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We studied the behavior of the cellular immune system in two types of carcinoma of frequent clinical occurrence: large bowel and gastric cancers. We contrasted both types of neoplasia with respect to the behavior of cellular immunity in the preoperative period, according to the evolutive stage of the tumor. Two hundred and nine subjects were studied: 86 with gastric adenocarcinoma, 63 with adenocarcinoma of colon-rectum and 60 healthy controls. Total lymphocytes were quantitated, as were B and T cell subpopulations, and T cell function by measuring the response to PHA. Summarizing the results, a clear depression in cellular immunity was observed in stage III of gastric cancer. In large bowel cancer the repression of cellular immunity is of lesser magnitude than in gastric cancer.
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PMID:Immunologic study of carcinoma of the digestive tract. Effects of the tumor stage. I. Cellular immunity in the preoperative period. 708 Sep 94

The effects of two immunomodulators, levamisole and lentinan, on cell-mediated immunity and regional lymph nodes of patients with gastric cancer were studied, including reference to some literature. 1) The counts of active T-cells and total T-cells in the peripheral blood showed a tendency of increase after administration of levamisole. 2) PHA lymphocyte blastoformation in the peripheral blood with and without autoserum was enhanced by the administration of levamisole and lentinan. 3) The percentage of active T-cells and PHA lymphocyte blastoformation in the peripheral blood with and without autoserum in proximal regional lymph nodes were increased by the administration of levamisole and lentinan. 4) The percentage of active T-cells and PHA lymphocyte blastoformation in the peripheral blood with and without autoserum in distal regional lymph nodes were increased by the administration of levamisole. 5) Postoperative depression of PHA and PPD skin tests was enhanced by preoperative administration of levamisole, and that of PHA skin test was enhanced by the administration of lentinan.
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PMID:[Preoperative immunochemotherapy for gastric cancer patients--immunomodulating effect of levamisole and lentinan on cell-mediated immunity and regional lymph node]. 718 39

To obtain information regarding the immunologic capacity of uninvolved regional lymph nodes (RLNs) draining tumor, the in vitro response of regional lymph node cells (RLNCs) to PHA was investigated in lymph nodes from 55 patients with gastric or colorectal cancer, and gastric ulcer. Evaluation of data relative to cancer progress demonstrated that, in gastric cancer patients, the response of stimulated RLNCs from patients with middle stage carcinoma was significantly higher compared with those of cells from patients with early or late stage carcinoma, such being similar to the response of RLNCs from control patients with gastric ulcer. The results from colorectal cancer patients were also similar to those from patients with gastric cancer. Our results suggest that RLNs without metastasis contain cells capable of responding to PHA despite the presence of growing tumors in these cancer patients. In the results of PHA stimulation in relation to the lymph node morphology, we observed in both groups of patients with gastric or colorectal cancer that, regardless of the extent of the cancer lymph nodes with lymphocyte predominance there was a high PHA response while lymph nodes with lymphocyte depletion and unstimulated nodes exhibited a low PHA response. These results show a close relationship between this lymph node morphology and immunologic status of nodes.
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PMID:Immunomorphologic study of regional lymph nodes in cancer: response of regional lymph node cells from gastric and colorectal cancer to PHA stimulation. 721 98

Sequential evaluation of lymphocyte blastogenic response (LBR) to PHA was performed in 10 melanoma patients and in 10 gastric cancer patients undergoing radical operations. Preoperative determinations showed a significant depression of LBR in both patient groups as compared to healthy controls. In patients operated for melanoma the average duration of anesthesia was 101 minutes and in patients who underwent gastric resection it was 192 minutes. In both patient groups a further significant depression of LBR was observed in the early postoperative period; however the LBR returned to preoperative levels more promptly in patients who underwent melanoma excision than in those who underwent gastric resection.
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PMID:[Sequential evaluation of lymphocytic blastogenesis in cancer patients after surgical treatment]. 731 75

The influence of splenectomy on the immunologic status of stomach cancer patients was studied. Eight gastric cancer patients having undergone total gastrectomy combined with splenectomy were evaluated immunologically. Peripheral lymphocyte counts were depressed and continued to be so in splenectomized patients. PHA-induced lymphocyte blastogenesis decreased rapidly after total gastrectomy with splenectomy and thereafter increased gradually. PHA skin test seemed to take almost the same time course. These recoveries to the normal range were observed 4 weeks after operation. In 10 other patients who had undergone total gastrectomy with splenectomy for gastric cancer and had been recurrence-free for more than 6 months, peripheral lymphocyte and T lymphocyte counts significantly increased compared with 7 cases having undergone total gastrectomy only, though no difference was seen in both PHA skin test and PHA-induced lymphocyte blastogenesis between the 2 groups.
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PMID:Clinical studies on the correlation between immunologic status and total gastrectomy combined with splenectomy. 743 81

The data from 329 gastric cancer patients (206 males and 123 females) were applied to the following statistical analysis. The stage of gastric cancer progress, which was determined by the general rules for the gastric cancer study in Japan, the counterpart of the TNM classification was predictable by a multi-variative mathematical model based on Hayashi's quantification theory which allowed to use qualitative variables as well as quantitative ones for the calculation using the variables relevant to clinical findings consisted of the grade of surgical operability, the grade of histopathological change, positive or negative liver metastasis, positive or negative histopathologically detectable lymph-node metastasis and so on. The variables relevant to clinical findings predicted accurately the stage by the above-cited model and multi-variative correlation coefficient (R2) was 0.9475, suggesting that 95% of the values predicted by those variables could identical to the observed value of the cancer stage. The variables relevant to clinical findings contributed only 29% (R2 = 0.2902) to the prediction of the histopathological grade. The stage and the histopathological grade also were predictable with the multi-variative regressive equations using the data of the clinico-pathological examinations which were administered on the day before the operation to 239 patients (139 males and 95 females) of gastric cancer and 82 control surgical patients (50 males and 32 females). The clinico-pathological indicators consisted of the SI values of Con A and PHA, leukocytes' count, lymphocytes' count, serum albumin concentration, B- and T-cell numbers. The factors which contributed to the stage, or the histopathological grade of gastric cancer were extracted respectively through principal component analysis using the respective correlation matrices consisted of the variables used for the calculation of the multi-variative regression equations in order to predict the stage or histopathological grade. For the male patients, the aging factor contributed to both of the stage and the histopathological grade. For the female patients, the factor relevant to the complication such as infectious diseases and low-nutrition emaciating the patient contributed to the cancer stage and the factor relevant to T-lymphocyte function contributed to the histopathological grade.
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PMID:[Studies on the classification of clinical stage and histopathological grade of gastric cancer, the contribution of clinical findings and clinico-pathological changes including immunological responses]. 772 75

The suppressive activity of immunologic suppressor factors (ISF) in the sera of 34 patients with ovarian cancer was investigated by using the lymphocyte proliferation and inhibition test. It was found that the sera of the preoperative patients could significantly suppress PHA-induced lymphocyte response, and that the suppression was dose-dependent. When the sera were diluted to 1 : 4,000, the suppressive activity could still be demonstrated. We also observed the suppressive activities in the sera of patients with cervical cancer, endometrial cancer, gastric cancer and lung cancer, and compared them with ovarian cancer patients. The observation showed that the suppressive activity in the sera of preoperative ovarian cancer patients was higher than that in the sera of gastric cancer or lung cancer patients. These results suggested that ISF played an important role in the development of ovarian cancer.
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PMID:[Immunologic suppressor factors in sera of patients with ovarian cancer]. 800 13

Glycoproteins from tumor cells isolated by Con A, PHA-E and RCA1, were coupled to horse radish peroxidase and then used as reporters to evaluate the oligosaccharides change on serum glycoproteins in digestive tract diseases and individual healthy persons. A value 2 standard deviation above the mean of the healthy person group was considered positive. The results indicated that the positive rates of the oligosaccharides bound with Con A, PHA-E and RCA1 were 51% (61/119), 70.6% (84/119), and 76.4% (91/119) in patients with gastric cancer; 53.3% (16/30), 46.7% (14/30) and 66.7% (20/30) in esophageal cancers; 28.5% (15/49), 49.0% (24/49) and 46.9% (23/49) in colorectal cancer; 78.1% (25/32), 81.3% (26/32) and 31.3% (10/32) in gallbladder tumors; 60.4% (29/48), 54.1% (26/48) and 39.5% (19/48) in hepatocellular cancer. The positive rates among the patients with benign diseases were also found to be 12.7% (16/126), 15.5% (20/126) and 26.2% (33/126). However, the expression of the oligosaccharide moieties recognized by WGA on the glycoprotein bound with Con A, was significantly lower in the serum of patients with tumors than in those with benign disease and in healthy individuals (P < 0.01, P < 0.01).
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PMID:Analysis of the N-glycosylation of the serum glycoproteins defined by Con A, PHA-E, RCA1 and WGA in Chinese patients with gastrointestinal diseases. 920 17

The acute phase protein, alpha1-acid glycoprotein (AGP), is a normal constituent of human blood (0.2-1 mg ml(-1)) and its glycosylation and concentration in the blood change during inflammation. In this review of our recent work, we discuss the immunomodulatory properties of AGP in connection with the structure of its carbohydrate chains. AGP samples prepared from normal donor serum (nAGP), serum obtained during abortion (fAGP), serum of cancer patients (cAGP), and ascitic fluid of patients with stomach cancer (sAGP) were subjected to analysis. All the samples except for fAGP had five N-linked chains of the 'complex' type, however, the numbers of bi-, tri-, and tetra-antennary chains, as well as glycan structures terminating these chains, were different. fAGP had three N-linked chains of the lactosamine and polylactosamine type and three O-chains which were not present in AGP isolated from the other sources. The glycoforms of nAGP and sAGP that were isolated using a ConA affinity column were similar in respect to their branching, but differed in their terminal oligosaccharides. sAGP was enriched in units ending in Le(x) and asialoagalacto (GlcNAc-terminating) forms. Immunomodulatory activity of different AGP preparations was tested in vitro by measuring their effect on the proliferative response of human lymphocytes stimulated by PHA, and by determining their influence on the production of IL-1, IL-2, IL-6, and TNF in the stimulated cells. nAGP was less active compared to cancer or fetal AGP in the proliferation test, but more active in affecting cytokine production. Some AGP glycoforms had opposite immunomodulatory effects. A new approach was developed in order to clarify the role of carbohydrate chains in the biological activity of AGP. A pool of N-linked oligosaccharide chains were attached to a soluble polyacrylamide matrix. This 'pseudoglycoprotein' was similar to AGP in its molecular weight; in its relative amounts of tetra-, tri-, and bi-antennary chains; and in the content of mono-, di-, tri-, and tetra-sialylated-oligosaccharides. This pseudo-AGP displayed a similar activity to its parent AGP in the biological tests. Analytical flow cytometry of leukocyte subpopulation from human peripheral blood showed that monocytes and granulocytes but not lymphocytes were the main targets for the binding of AGP and pseudo-AGP. This binding was inhibited by synthetic glycoconjugates containing mannose or sialic acid. The binding curve data suggested that there are two monocyte and granulocyte populations. These may have different carbohydrate specificities. All the evidence provided by these studies indicate that it is the carbohydrate chains on AGP that are important in modulating the immune system and not the AGP molecule itself.
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PMID:Carbohydrate composition and immunomodulatory activity of different glycoforms of alpha1-acid glycoprotein. 929 96

We have taken advantage of a recently described technique of transformation and immortalization of T lymphocytes using the lymphotropic Herpesvirus saimiri, to achieve long-lasting T-cell lines from gastric cancer patients and healthy volunteers. Blood samples were drawn and T lymphocytes were transformed. Once sustained growth was observed, lines were subjected to phenotypic and functional analyses, and the results compared with freshly isolated peripheral blood mononuclear cells. Cytofluorometric analysis revealed that CD3 and CD45 were found at lower proportion in primary cells from patients than from control individuals (54% vs 75%, p<0.001, 90% vs 96%, p<0.05, respectively), and in HVS-derived T-cell lines (90% vs 98%, p<0.05, 97% vs 100%, p<0.05, respectively). Proliferative analyses showed that primary isolated cells were unable to respond adequately to CD3-, CD2-, and PHA-mediated stimulation, as compared to controls. Similarly, T-cell lines from patients proliferated to a lesser extent when CD3- and CD2-mediated stimuli were considered, especially when simultaneous stimulation via CD3 and CD2 molecules was carried out (47,824 counts per minute [cpm] vs 121,478 cpm, p<0.05). Altogether these results show that the defects reported in T cells from patients with cancer are not exclusively due to tumour-derived factors, since the alterations persist in long-lasting, HVS-transformed, T-cell lines, suggesting that this model seems a suitable one to disclose them.
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PMID:Intrinsic defects explain altered proliferative responses of T lymphocytes and HVS-derived T-cell lines in gastric adenocarcinoma. 1283 Mar 24


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