Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0024623 (gastric cancer)
 36,219 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A 31-month-old female mongrel dog was orally administered with 50 mg or 100 mg of N-nitrosobutylurea (NBU) in gelatin-capsule 3 times per week for 19 months with interposing periods of complete suspension. Thirty-four foci of signet ring cell carcinoma were found in the antral region of the stomach. The majority of the foci (31 foci) were early cancer, and the remaining foci were invasive cancer. In addition to these lesions, there was "a single gland cancer" in which a row of cancer cells was confined to a single gland. The whole gland was composed of two cell layers; the inner layer facing the lumen was normal gastric cells and the outer layer was atypical or neoplastic cells underlaid by the basement membrane. Mitosis was frequently observed on the bottom of the gland. Atypical or neoplastic cells seemed to mature gradually through a process of upward migration with increase in cytoplasmic Alcian blue-PAS and HID-AB positive mucin. Some of the cells rich in mucin moved into the lamina propria. The other cells remained in the flow of the regular cell renewal system of the normal gastric cells and reached the top of the gland. This observation revealed a mode of incipient gastric cancer growth, which starts and spreads within a single gland, before it invades the surrounding lamina propria.
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PMID:A mode of incipient growth in chemically induced signet ring cell carcinoma of the canine stomach. 22 34

Investigations of Culling et al. showed the possibility of using KOH/PAS effect (visualisation of C7 and C8O-acylated sialic acids characteristic to colon mucin) for differential diagnosis of metastatic colorectal cancer. Our investigations revealed, however, KOH/PAS positive mucin prevailing in some cases of gallbladder, pancreatic and gastric cancer. This makes serious limitation of Culling method in the routine diagnostic work.
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PMID:Limitations of Culling PATS/KOH/PAS method of differential diagnosis of metastatic colorectal cancer. 74 35

Despite the importance of in vitro study of gastric cancer, there are very few established cell lines derived from human gastric carcinoma. We have recently established a new cell line derived from human gastric cancer which has the ability to produce tumor markers. This cell line has been designated JR-St. This cell line was derived from the cerebrospinal fluid of a 37-yr-old female patient who had metastatic brain tumor of signet ring cell gastric adenocarcinoma. This cell line has been maintained for more than 24 months through 80 passages with stable growth. PAS staining showed intracellular mucin granules. Transmission and scanning electron microscopy revealed cells with numerous microvilli and fine projections as well as intracellular granules, indicating mucin. This cell line had the ability to produce high concentrations of tumor markers such as carcinoembryonic antigen (CEA) and carbohydrate antigen (CA) 19-9. Thus Thus this cell line should provide a very useful tool for the investigation of gastric cancer such as analysis of tumor markers as well as effects of anti-cancer drugs or growth factors.
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PMID:Characterization of a newly established cell line (JR-St) derived from human gastric signet ring cell cancer, producing tumor markers. 184 29

Despite the importance of in vitro study of gastric cancer, the established cell lines derived from human gastric carcinoma are very few. We have recently established a new cell line derived from human gastric cancer which has the ability to produce several tumor markers. This cell line has been designated JR-1. The cancer cells were obtained from the cerebrospinal fluid of a 37 year-old female patient who had metastatic brain tumor of the poorly differentiated gastric adenocarcinoma. The cells were inoculated into the tissue culture flask containing Ham's F-12 medium supplemented with 10% fetal bovine serum, antibiotics. Within 24 hours, the cells attached to the surface of the flask and started to grow. The first subcultures were performed at 1 week, and subsequent subcultures have been done once a week. This cell line has been maintained for more than 15 months through 60 passages with a stable growth. Chromosome analysis of the cells was performed. The doubling time of the 20th passage was 72 hours. Under phase contrast microscopy, monolayered pavement-like cell arrangement was observed. PAS staining showed intracellular mucin granules. Transmission and scanning electron microscopy revealed spindle-shaped cells with numerous microvilli and fine projections as well as intracellular granules, indicating mucin. Tumor markers produced by this cell line were CEA, CA19-9, TPA and Procollagen III.
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PMID:[Establishment and characterization of a tumor marker producing cell line (JR-1) derived from a gastric scirrhous cancer]. 256 3

The concept of PASK (Patient follow-up System of Kyushu University Hospital) has been innovated in order to manage clinical data of patients in the hospital-wide scope over long periods of time by using computers. PASK represents a collection of subsystems, each tuned to a specific disease. For the initial implementation, the subsystem for stomach cancer (STOMAC) was constructed according to the Cancer Center of Kyushu University Hospital project. STOMAC began to work in October 1984 and has proved effective. Using STOMAC, doctors in a clinical section can easily access patient data gained in another section. Besides providing a data reference function, STOMAC automatically gives alarms for abnormalities in clinical examination results, anti-tumor drug dosage, visitation cycles of patients, etc. The database and STOMAC programs are strongly standardized and easily applicable to another specific disease.
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PMID:A patient follow-up system for stomach cancer. 302 82

Dysplasia cancer sequence has not been determined in gastric cancer yet. Dysplastic changes are not frequent. Gastric cancer generally develops in areas of chronic atrophic gastritis. This chronic atrophic gastritis (CAG) is often associated with intestinal metaplasia (IM). IM has been classified in three types, according to morphologic patterns, differentiation and mucins production. We reviewed 55 gastrectomy specimens and 278 endoscopic biopsies. In order to determine an histological high-risk group, we chose cases with preneoplastic conditions (60 CAG, 10 biopsies of gastric remnants, 3 flat adenomas and 55 gastrectomies by cancer or ulcer). We also included 12 hyperplastic polyps because they may contain foci of intestinal metaplasia. Mucin techniques (PAS-ALCIAN BLUE Ph 2.5 and HID-A.B) were used in all cases that showed extensive intestinal metaplasia. In addition, we used immunohistochemistry techniques to detect CEA. Dysplasia was found only in flat adenomas (3 cases), early gastric cancer (1 case) and advanced cancer (3 cases). We considered a preneoplastic lesion only to moderate or severe dysplasia. Hyperplastic regenerative pathology is considered a reversible condition. Therefore, it should be differentiated from dysplasia. We found that IM type III (sulfomucin predominance) is the most related to carcinoma, particularly to the intestinal type. CEA antigen is poorly specific in detecting high-risk lesions because it was seen in regenerative pathology and in gastric cancer too. Relationship of dysplasia and carcinoma, and/or neoplastic polyps was similar to other series. Concerning to follow-up items, we agree with the concepts proposed by the Japanese Research Society for Gastric Cancer.
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PMID:[Gastric dysplasia: its incidence in precancerous conditions, advanced cancer and early cancer]. 325 19

In this report, 141 patients with gastric cancer were studied histochemically. Tissue CEA was stained by the CEA-PAP method and the gastric cancer was classified into CEA-producing (96 cases, 68.1%) and CEA non-producing gastric cancer (45 cases, 31.9%). Histologically, CEA-producing gastric cancer was well differentiated adenocarcinoma and CEA non-producing gastric cancer was chiefly undifferentiated carcinoma. PAS, pH 2.5 Alcian-blue, High Iron Diamine, Alkaline-PAS, and Concanavalin A paradoxical stain were applied to specimens from each type of gastric carcinoma. Mucosubstances of CEA-producing gastric cancer were positive for A-B, HID, AL-PAS and CPS III-1; those of CEA non-producing gastric cancer were positive for PAS and CPS III-s, but negative for A-B, HID and A1-PAS. These results suggest that CEA-producing gastric cancer arises from intestinal metaplasia of gastric mucosa and that CEA non-producing gastric cancer arises from the gastric mucosa itself.
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PMID:[Mucohistochemical studies of CEA producing and non-producing stomach cancers]. 619 17

PCAA, a glycoprotein antigen fractionated from the ascites fluid of a patient with pancreatic cancer and sharing an identical immunogenicity with Gelder's POA, and PaA, a novel pancreatic tissue antigen, were studied immunohistologically. Serial paraffin sections were prepared from surgical specimens of 11 cases of pancreatic cancer, 15 of gastric cancer, 12 of colonic cancer, and 2 of gallbladder cancer; these were then subjected to immunofluorescence and immunoperoxidase stainings. In noncancerous tissues, PCAA was detected unexpectedly at goblet cells of the whole intestine, but was completely absent in pancreatic tissues, while PaA was not demonstrated in intestinal tissues, but was positive at acinar cells of the pancreas. In pancreatic cancer tissues, PCAA and PaA were detected at apical cytoplasm of cancer cells, although positive cells were in different proportions and had different distributions. PCAA-positive cells were mucin-producing (positive in PAS-alcian blue staining) and were well differentiated histologically, while PaA-positive cells were less differentiated and poor in mucin production. Among 11 cases of pancreatic cancer, both PCAA- and PaA-positive cells were demonstrated in 3 cases, PCAA-positive cells alone were found in 3 cases, and PaA-positive cells alone were seen in 4 cases. In 27 gastrointestinal cancer tissues, PCAA was detected in 7 cases of mucin-producing cancer, and PaA was demonstrated in 2 cases of poorly differentiated adenocarcinoma.
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PMID:Differential distribution of the pancreatic cancer-associated antigen (PCAA) and pancreatic tissue antigen (PaA) in pancreatic and gastrointestinal cancer tissues. 636 96

A female patient of 18 with Peutz-Jeghers syndrome and gastric cancer was found to have in macroscopically intact areas of gastric mucosa cysts of the body of the gastric mucosa glands (the first observation in the national literature) which were characterized by glandular structures of various sizes lined with clear columnar epithelium with basally located nucleus and with production of the PAS-positive substance. Some of the cysts extend into the gastric lumen, some form polyps. They should be regarded as hamartomas. When the cystic epithelium undergoes malignant transformation, the trend for mucus formation is not lost.
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PMID:[Cysts of the body of gastric mucosa glands in a female patient with Peutz-Jeghers syndrome and stomach cancer]. 684 4

One hundred and fourty seven lymph nodes involved by metastases of the stomach, breast and lung cancer have been examined. It was found that in metastatic stomach cancer the PAS-reaction intensity is high, the mitotic level is low, the modal class of cells on the histogram is diploid. Breast cancer metastases are characterized by weak PAS-reaction, distinct stroma and cell reaction around the tumor, the prevalence of tetroploid nuclei on the histogram. In lung cancer metastases the PAS-positive substances are practically absent, the stroma and cell-reaction around the tumor is less distinct, the mitotic level is high, the number of hypodiploid nuclei on the histogram is 10.4%. Based on the data obtained histographically the author suggests a new criterion -- a coefficient of stability/a ratio of the diploid nuclei number to the number of the rest ones/ which allows a quantitative characterization of the intensity of the nuclei polymorphism in tumors.
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PMID:[Comparison of the morphology of stomach, breast and lung cancer metastases to the lymph nodes]. 700 44


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