Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0024623 (gastric cancer)
 36,219 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In an attempt to determine the significance of increased beta-glucuronidase content of gastric juice of patients with gastric carcinoma, gastric mucosal cells were exposed, in organ culture technique, to a known gastric carcinogen and indices of carcinogenic activity were studied in the ambient fluid and in the mucosal cells. Isotopic methods were used to determine cell viability. Indices of carcinogenic activity in the ambient fluid were beta-glucuronidase and lactate production and changes in the LDH isoenzyme pattern of the homogenates of the exposed cells were also studied. Incubation with the carcinogen resulted in increased production of beta-glucuronidase and lactate, suggesting the increased beta-glucuronidase activity in the gastric juice of patients with gastric cancer indicates malignancy.
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PMID:beta-Glucoronidase and the gastric epithelial cell. A study using organ culture. 61 35

The activity of acid phosphatase, beta-glucuronidase and N-acetyl-beta-glucosaminidase was assessed using semiquantitative cytochemical methods in peripheral blood lymphocytes of 45 untreated patients with gastric cancer and 80 healthy subjects. In cancer patients the study demonstrated a statistically significant decrease in the number of lymphocytes with granular reaction for acid phosphatase and N-acetyl-beta-glucosaminidase, as well as an increase in the number of lymphocytes showing a granular-diffuse reaction for the above enzymes and a diffuse reaction for all the studied lysosomal enzymes. Possible mechanisms of the observed changes are discussed.
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PMID:Lysosomal enzymes in peripheral blood lymphocytes of patients with gastric cancer. 243 66

Flow cytometric DNA studies were performed on cell suspensions of biopsy specimens from gastric tumors and neutral gastric mucosa in 18 patients with gastric cancer and 9 patients with gastric polyps. In cancer, aneuploidy was found in two tumors in the antral and five in the body part of the stomach (39%). The mean DNA index for aneuploid cancers was 1.57. In patients with aneuploid carcinomas three biopsy specimens from uninvolved mucosa also showed aneuploidy. In diploid carcinomas in the antral part of the stomach, the proliferative index (PI) was increased when compared with that of antral mucosa in controls (p less than 0.05). Increased PI was found in uninvolved mucosa in aneuploid carcinomas of the body part of the stomach when compared with that in diploid carcinomas (p less than 0.001). In uninvolved body mucosa in aneuploid carcinomas of the body part significantly reduced levels of acid-beta-glucuronidase (p less than 0.0001) were found when compared with diploid carcinomas. No polyps showed aneuploidy, and the PI in biopsy specimens from patients with gastric polyps did not differ from that in controls.
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PMID:Flow cytometric DNA studies in human gastric cancer and polyps. 343 17

Levels of lactic dehydrogenase and beta-glucuronidase were measured in gastric wash samples obtained from 445 patients over 40 years old attending a routine diagnostic endoscopy clinic. An index was derived from the two levels and used as a test for the presence of gastric cancer. Of the 24 patients with an endoscopic diagnosis of gastric cancer, 21 (91.3%) proved positive on this test, including all four cases of early gastric cancer, which were found at repeat endoscopy. The specificity of the test for gastric cancer was 81.3%. Among those patients with false positive results who had endoscopic biopsy were four out of the five cases of severe dysplasia, and four out of the 13 cases of type 2B intestinal metaplasia. Atrophic gastritis alone or with intestinal metaplasia was found in 95.9% of the false positives who were biopsied.
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PMID:Gastric enzymes as a screening test for gastric cancer. 357 36

Lactic dehydrogenase (LDH) and beta-glucuronidase (bGLU) levels are elevated in the gastric juice of patients with gastric cancer, and these enzymes have been used as a screening test for gastric cancer. False positives are common, however, and we have measured enzyme levels, including LDH isoenzyme fraction, in tissue homogenates from resected stomach specimens and compared these with histological features. Increases in bGLU were found in carcinoma and chronic gastritis, an increase in LDH was found in carcinoma, whilst mLDH fraction was increased in chronic gastritis, carcinoma and intestinal metaplasia, particularly the sulphomucin variant. These findings indicate the basis for the screening test, and also the reason for the false positives. An elevated mLDH fraction may be a useful marker of a group at increased risk of developing gastric cancer, and worthy of long-term follow-up.
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PMID:Beta-glucuronidase, LDH and LDH isoenzyme levels and screening for gastric cancer. 375 63

Lactic dehydrogenase (LDH) and beta-glucuronidase concentrations were measured in the resting gastric juice of 113 patients presenting with dyspepsia. All patients were investigated by double-contrast barium meal, endoscopy with biopsy, and, when appropriate, by laparotomy. In all patients tested there was a positive correlation between LDH and beta-glucuronidase concentrations. An index derived from the two enzyme activities correctly predicted the presence of gastric carcinoma in 41 out of 42 cases, and identified the only 2 cases of early gastric cancer in the series. There were 13 (11.5%) false-positive results, all in cases with extensive intestinal metaplasia, a change which may be associated with an increased risk of gastric malignancy. The measurement of gastric-juice enzymes is useful in the diagnosis of gastric cancer and may be of value in the identification of high-risk groups. The test is easily performed, inexpensive, and reproducible.
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PMID:Gastric-juice enzymes--an aid in the diagnosis of gastric cancer? 611 86

Measurement of beta-glucuronidase and lactic dehydrogenase in the fasting gastric juice of dyspeptic patients is a useful test for gastric cancer, but about 10% of patients tested have positive results without a demonstrable carcinoma. We have compared the histological features of multiple endoscopic gastric biopsies from 17 such patients with apparently false positive enzyme tests with gastric biopsies from 17 age and sex matched patients with negative enzyme tests. Epithelial dysplasia, a precancerous lesion, was found in 3 patients with positive enzyme tests but was not found in those with negative enzyme tests. Sulphomucin-containing intestinal metaplasia, another lesion which is associated with carcinoma of the stomach, was found in 8 patients with a positive enzyme test (including all 3 with dysplasia) but in only one patient with a negative enzyme test. These findings suggest that patients with positive gastric juice enzyme tests who do not have an established carcinoma form a group who are at increased risk of developing gastric cancer in the future and who may be worthy of long-term follow-up.
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PMID:Elevated gastric juice enzymes--a marker for increased gastric cancer risk? 650 14

Activity of acid phosphatase, beta-glucuronidase and N-acetyl-beta-glucosaminidase was investigated cytochemically in neutrophils from peripheral blood of 22 untreated patients with gastric cancer, 8 patients with cancer of large intestine and in 40 healthy individuals. Differences in the activity of enzymes studied were demonstrated between patients at different clinical stages of cancer advancement, as well as between cancer patients and healthy subjects. Most significant changes were observed in patients with initial (1st stage) cancer, as compared with the control group, including decrease of acid phosphatase and beta-glucuronidase activity, accompanied by an enhanced N-acetyl-beta-glucosaminidase activity. The possible mechanism of these changes is discussed.
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PMID:Activity of some lysosomal enzymes in neutrophils from peripheral blood of patients with digestive tract cancer at different clinical stages of the disease. A cytochemical study. 718 22

N-Acetyl-beta-glucosaminidase (NAG) and beta-glucuronidase were measured in the serum of 70 patients with breast and digestive-tract neoplasms and in 70 healthy subjects. The mean value of the NAG activity was significantly (P < 0.001) elevated in patients with gastric, liver and pancreas cancer as compared with the reference population. In patients with liver and pancreas cancer the very high sensitivity contrasted with a low specificity. NAG elevations above normal were observed in 14 (78%) patients with breast cancer, in 11 (100%) with gastric cancer, in 17 (70%) with colorectal cancer, in 8 (100%) with liver cancer and in 9 (100%) with pancreas cancer. In patients with breast and gastric cancer the enzyme shows a good specificity and sufficient sensitivity as a tumor marker. beta-Glucuronidase appeared less sensitive and was significantly elevated (100%) only in patients with pancreas cancer.
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PMID:A study of serum glycosidases in cancer. 786 Jun 21

In the present study we measured the activities of the following enzymes: LDH (lactic dehydrogenase), beta-glucuronidase, acid maltase, phosphohexoseisomerase (PHI) and acid proteases in the gastric juice of patients with gastric cancer (n = 50) (Case Group), in endoscopically normal subjects (n = 50) and in subjects with different non tumor-like digestive pathologies (n = 55) (Control Groups). In the patients with gastric carcinoma we found a significant increase in LDH, beta-glucuronidase, PHI and acid maltase activities and a decreased activity of acid proteases. The results agree with previous findings from other workers. The variations of enzyme activities in gastric juice can help to differentiate between malignant and benign processes of the gastric mucosa.
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PMID:[Enzymes in gastric juice. An aid in the diagnosis of gastric cancer]. 943 22


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