Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0024623 (gastric cancer)
 36,219 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A novel method has been developed to quantify alpha(1----3)-L-fucosyltransferase activity in human sera by applying a sandwich-type immunoradiometric assay. H type 2 trisaccharide (6Fuc alpha 1----2Gal beta 1----4GlcNAc) covalently attached to bovine serum albumin (BSA) was used as an acceptor and incubated with serum samples in the presence of guanosine diphosphate-fucose. The resulting product, Y tetrasaccharide (Fuc alpha 1----2Gal beta 1----4[Fuc alpha 1----3] GlcNAc beta-BSA), was detected by a sequential use of anti-BSA antibody-coated bead and 125I-labeled anti-Y antibody. Inter- and intra-assay CVs for alpha(1----3)-L-fucosyltransferase were both less than 4%, and the results of the dilution linearity and analytical recovery studies were satisfactory. Using the present assay method, we measured alpha(1----3)-L-fucosyltransferase in serum from patients with benign and malignant gastric disorders and in healthy subjects. The detection rate of alpha(1----3)-L-fucosyltransferase for cancer was apparently higher than that of carcinoembryonic antigen measured in the same samples, particularly in the early clinical stage; indeed, no correlation was observed between the concentrations of the two potential markers. The results indicate that the present assay method seems to be excellent for the determination of serum alpha(1----3)-L-fucosyltransferase activity and useful for the detection of cancer-associated increases of the enzyme activity at the early stage of gastric cancer.
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PMID:Novel method for quantifying alpha(1----3)-L-fucosyltransferase activity in serum. 176 83

Two tumour markers, immunosuppressive acidic protein (IAP) and carcinoembryonic antigen (CEA), were assayed in gastric cancer patients. Levels of IAP and CEA were measured simultaneously in the preoperative and postoperative periods. The usefulness of the combined assay of these markers for detection of recurrence of cancer was investigated in terms of sensitivity, specificity and diagnostic accuracy. Sensitivity was not high (69.2%), but specificity and diagnostic accuracy were 96.7% and 86.9%, respectively. In cases with metastases in the liver, sensitivity (100.0%), specificity (100.0%) and diagnostic accuracy were high. In cases of peritoneal dissemination, these indices were low. The combination assay of IAP and CEA appears to be useful for detection of recurrence of gastric cancer, especially in patients with liver metastases.
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PMID:Diagnostic accuracy of combination of assays for immunosuppressive acidic protein and carcinoembryonic antigen in detection of recurrence of gastric cancer. 182 87

Despite the importance of in vitro study of gastric cancer, there are very few established cell lines derived from human gastric carcinoma. We have recently established a new cell line derived from human gastric cancer which has the ability to produce tumor markers. This cell line has been designated JR-St. This cell line was derived from the cerebrospinal fluid of a 37-yr-old female patient who had metastatic brain tumor of signet ring cell gastric adenocarcinoma. This cell line has been maintained for more than 24 months through 80 passages with stable growth. PAS staining showed intracellular mucin granules. Transmission and scanning electron microscopy revealed cells with numerous microvilli and fine projections as well as intracellular granules, indicating mucin. This cell line had the ability to produce high concentrations of tumor markers such as carcinoembryonic antigen (CEA) and carbohydrate antigen (CA) 19-9. Thus Thus this cell line should provide a very useful tool for the investigation of gastric cancer such as analysis of tumor markers as well as effects of anti-cancer drugs or growth factors.
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PMID:Characterization of a newly established cell line (JR-St) derived from human gastric signet ring cell cancer, producing tumor markers. 184 29

One-hundred and seventy-four consecutive patients who underwent curative resection for gastric and colorectal cancer between 1983 and 1985 were studied prospectively to evaluate the roles of sequential carcinoembryonic antigen (CEA), tissue polypeptide antigen (TPA) and Ca 19-9 determinations and independent clinical examinations, in the early diagnosis of resectable recurrences. Sixty-six recurrences (33 from gastric and 33 from colorectal cancer) were detected between 6 and 42 months after primary surgery. In gastric cancer CEA, TPA and Ca 19-9 showed a sensitivity of 64%, 73% and 60% respectively and a specificity of 67%, 65% and 54% respectively. Nine patients (27%) underwent surgical treatment for recurrent disease, and four of these (44.4%) had resectable recurrence, for a total resectability rate of 12%. Of these four patients, three are still living after 12, 36 and 44 months respectively from re-operation without evidence of neoplastic disease. In one of these patients, re-operation was performed on the basis of the elevation of the three markers, without any other clinical sign of disease. This patient had a resectable solitary hepatic recurrence. In colorectal cancer. CEA, TPA and Ca 19-9 showed a sensitivity of 73%, 73% and 49% respectively, and a specificity of 77%, 87% and 97% respectively. Fourteen patients (42.4%) underwent surgical treatment for recurrent disease and eight of these (57%) showed resectable recurrence, for a total resectability rate of 24.2%. Six patients are still living after 9, 16, 21, 31, 41 and 53 months respectively from re-operation without evidence of neoplastic disease.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Gastrointestinal cancer follow-up: the effectiveness of sequential CEA, TPA and Ca 19-9 evaluation in the early diagnosis of recurrences. 187 36

To establish further the clinical significance of the CA-195 tandem immunoradiometric assay in gastro-intestinal malignancies, the sera of a total of 222 subjects have been analysed and compared with assays of the "classical gastrointestinal tumour markers", CA19-9 and carcinoembryonic antigen (CEA). CA-195 elevations above normal (greater than 10 U/ml) were noted in 51/72 (70.8%) colorectal, 15/15 (100%) pancreatic, and in 6/12 (50%) gastric cancer patients. Whereas CA19-9 was increased (greater than 37 U/ml) in 65%, 93%, and 42% of cases, only 54% colorectal, 45% pancreatic, and 42% gastric cancer patients had pathologically elevated serum CEA levels (greater than 5 ng/ml). No abnormal increase of both CA-195 and CA19-9 was found in healthy volunteers, whereas 3/20 (smoking) individuals had CEA levels slightly above normal. With a 29% false-positive rate noted among 103 patients with benign gastrointestinal disorders, the specificity of CA-195 was superior to that of CA19-9 (58%) and comparable with that of CEA (31%). A significant correlation between CA-195 levels and the clinical/pathological stage of disease was noted in colorectal (P less than 0.01) and pancreatic cancer patients (P less than 0.007). Preliminary results of serial measurements of CA-195 in colorectal cancer suggest that this new marker protein, which has no cross-reactivity with CEA, may be useful as a non-invasive test for postoperative surveillance of patients to detect disease recurrence, and serve to complement (though certainly not replace) standard clinical measurements of response to chemotherapy.
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PMID:Comparative analysis of cancer-associated antigen CA-195, CA 19-9 and carcinoembryonic antigen in diagnosis, follow-up and monitoring of response to chemotherapy in patients with gastrointestinal cancer. 189 Jan 43

The immunoreactivity and affinity of seven kinds of monoclonal antibody raised against human gastric cancer (MKN-45) secreted carcinoembryonic antigen (CEA) were determined with the method of cell binding assay in vitro. Tumor localization and biodistribution of radiolabeled antibodies were performed in athymic mice implanted MKN-45 xenografts. Results obtained were as follows: 1) The affinity constant of CEA-specific three antibodies (1A4, 1B2, 4H11) was the same approximately, whereas the immunoreactivity found to be quite different among them. While CEA-nonspecific four antibodies (7D1, 6C7, 2C3, 5H7) showed the much higher affinity constant than that of the former. 2) In an animal model on tumor localization and biodistribution studies, CEA-specific antibodies obtained more highly tumor targeting and cleared more rapidly from the blood and non-tumor organs than CEA-nonspecific antibodies did, so that tumor to nontumor ratios was increased. 3) In this model system it is the immunoreactivity preparation of antibodies that improved tumor targeting and tumor activity retention, on the other side, the affinity constant of antibodies were associated with rapid clearance from the blood and non tumor sites. In conclusion, this studies would also be beneficial for practical use and clinical application of radiolabeled monoclonal antibodies.
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PMID:[Experimental study of the relationship between in vitro binding activity and in vivo tumor accumulation of radiolabeled anti-CEA monoclonal antibodies]. 189 Jul 53

Four human gastric cancer xenografts in nude mice were analyzed for tumor growth and associated levels of carcinoembryonic antigen (CEA) in sera and tissues. The xenografts were established from gastric cancer patients and transplanted serially over 20 passages in our laboratory. Histologically, two xenografts were intestinal type and the other two were diffuse type. All of them were positive for CEA immunohistochemically. Individual xenografts showed a positive correlation between tumor growth and serum CEA level, with correlation coefficients ranging from 0.73 to 0.91. Serum CEA levels rose continuously with increasing tumor weight after inoculation. A positive correlation was also observed between the tissue CEA level and tumor growth, the former increasing along with the latter, showing the correlation coefficients ranging from 0.69 to 0.81. Furthermore, the level of serum CEA closely paralleled to that of tissue CEA, showing the correlation coefficients ranged 0.54 to 0.90. The deviation of the ratio of serum CEA level/tissue CEA level was constant among the xenografts, although the mean ratio differed slightly. These results suggest that serum CEA level is closely correlated with tissue CEA level as well as tumor growth, and that the elevation of serum CEA is attributable to the tissue CEA level rather than tumor weight.
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PMID:[Changes in serum and tissue carcinoembryonic antigen with growth of human gastric cancer xenografts in nude mice]. 196 Nov 81

A new human gastric cancer cell line (OCUM-1), which was derived from Borrmann type IV tumor of the stomach, was established. The cell line grew sometimes singly and sometimes in clusters, and continued to grow for more than 3 years. It's doubling time was 33.2 hours, chromosomal mode was 50, and nuclear DNA ploidy pattern was diploid. The cells could grow in nude mice. It produced carcinoembryonic antigen, carbohydrate antigen 19-9, and cancer-associated antigen SPan-1 and expressed epidermal growth factor receptor. Supplementation of epidermal growth factor to culture medium increased the cell number statistically significantly. It was decreased by supplementation of chondroitin sulphate. So the cell line OCUM-1 might be useful for the study of gastric cancer, especially Borrmann type IV gastric cancer.
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PMID:[Establishment and characterization of a new gastric cancer cell line (OCUM-1), derived from Borrmann type IV tumor]. 196 Nov 83

We examined the correlation among preoperative serum carcinoembryonic antigen (CEA) levels, staining properties of the tumors by CEA immunohistochemistry and the tumorigenicity of their xenografts in nude mice, in 28 patients with gastric cancer. Eleven (40 per cent) of them were positive for serum CEA (greater than or equal to 2.5 ng/ml) and seven (25 per cent) of the xenografts were tumorigenic in nude mice. All the tumorigenic cases were positive for serum CEA (p less than 0.001) and the mean value of the serum CEA level in the patients with tumorigenic neoplasms was 20.8 ng/ml, being significantly higher than that (1.4 ng/ml) in the patients with non-tumorigenic neoplasms (p less than 0.001). Twenty-five of the 28 carcinomas (89 per cent) were positive for CEA staining in their cancer cells by the ABC method and CEA localization correlated with tumorigenicity (p less than 0.05). These results suggest that the serum CEA level in patients is correlated with the tumorigenicity of their gastric carcinoma xenografts in nude mice and may account for the poor prognosis of patients with high serum CEA.
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PMID:Serum CEA levels in patients with gastric carcinoma correlate with the tumorigenicity of their xenografts in nude mice. 204 Dec 38

We established a human gastric cancer xenograft which, when inoculated into nude mice, showed a positive correlation between tumor growth and the serum level of carcinoembryonic antigen (CEA). Serum CEA levels in the mice rose continuously with increasing tumor weight after inoculation, showing a correlation coefficient of 0.96. A positive correlation was also observed between the tissue CEA level and tumor weight, the former increasing along with the latter. Furthermore, the level of serum CEA closely paralleled that of tissue CEA. The serum CEA level fell after tumor extirpation, with a half-life of approximately 86 h. These results suggest that the elevation of serum CEA is attributable to the gain in tumor weight as well as the increase of CEA production in the tumor tissue. Thus, human gastric cancer xenografts in nude mice are a good model for examining the biological role of CEA.
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PMID:Changes in serum and tissue carcinoembryonic antigen with growth of a human gastric cancer xenograft in nude mice. 210 47


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