Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0024591 (malignant hyperthermia)
 2,353 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Binding of monoclonal antibody MHS-5 to western blots of human seminal plasma was employed to follow the fate of a seminal vesicle-specific antigen (SVSA) during semen liquefaction. Ejaculates from four vasectomized donors were collected in a manner to inhibit liquefaction or to allow liquefaction to proceed at room temperature. Aliquots of the liquefying seminal fluid were removed at specific time points and further liquefaction inhibited with sodium dodecyl sulfate (SDS). Western blot analysis using monoclonal antibody MHS-5 demonstrated that the SVSA epitope in all donors was located on multiple bands ranging in mass from 15-92 kDa in unliquefied ejaculates; immunoreactive peptides below 15 kDa were not detected. As early as 5 min post ejaculation, immunoreactive bands below 15 kDa were identified in liquefying samples. During the same time period (5 min), immunoreactive bands of 69-71 and 58 kDa could not be immunologically detected in liquefying samples. A decrease in immunoreactive staining of components higher molecular mass was accompanied by a concomitant increase in immunoreactive staining of intermediate and small molecular mass molecules during the first 2 h of liquefaction. After 8-24 h of liquefaction, two immunoreactive bands of 10.9 and 12.5 kDa predominated. Between 24 and 48 h, each donor's ejaculate demonstrated a common single immunoreactive band of 10.9 kDa. These results indicate that there is a rapid transformation in mass of the SVSA with major 69-71 and 58 kDa bands being converted to forms of lower mass within 5 min of ejaculation.
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PMID:Human seminal vesicle-specific antigen during semen liquefaction. 367 97

The location of the antigen recognized by monoclonal antibody MHS-5 in the human reproductive tract was examined by means of enzyme-linked immunosorbant assay (ELISA) and indirect immunohistochemistry employing the strepavidin-biotin-complex method. Homogenates of male reproductive tract tissues and other human organs assayed by ELISA demonstrated immunoreactivity of the MHS-5 monoclonal antibody specifically with human seminal vesicle extracts. Varying ratios of seminal protein and monoclonal antibody ascites were tested to determine the amount of antigen necessary to completely absorb the antibody in the ELISA assay. This ratio was subsequently used to obtain the absorbed negative control for histochemical localization studies. By light microscope examination of seminal vesicle tissue in paraffin section, the MHS-5 antigen was localized in principal cells of the seminal vesicle epithelium. Epididymal sperm, obtained from patients at orchiectomy and vasovasostomy were found to lack the MHS-5 antigen. Following incubation with seminal protein or fluid obtained from the lumen of the human seminal vesicle, epididymal sperm reacted with the MHS-5 antibody on ELISA. These findings indicate that the MHS-5 antigen, a novel protein previously shown to be a unique marker for human semen, is a secretory product of the human seminal vesicle epithelium and may be reconstituted on the surface of epididymal spermatozoa.
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PMID:Immunohistochemical localization of the MHS-5 antigen in principal cells of human seminal vesicle epithelium. 370 82

In vitro contracture tests, in which muscle specimens are exposed to halothane or caffeine are, at present, the only generally accepted screening methods for the diagnosis of susceptibility to malignant hyperthermia (MHS). Static tests (performed with the muscle held at constant length) are used more commonly although, in addition, some MH investigation units use dynamic tests, in which the length of the specimen is varied. We have performed dynamic and static tests in parallel on muscle from 112 patients. The dynamic halothane test was more sensitive in discriminating between MHS and MH negative (MHN) individuals than the static halothane test. However, the dynamic caffeine test was less sensitive at discriminating between MHS and MHN individuals, and nothing is to be gained by including it in the investigation.
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PMID:Static v. dynamic tests in the in vitro diagnosis of malignant hyperthermia susceptibility. 370 2

Studies of whole-kidney function and micropuncture measurements in superficial nephrons were performed to investigate the role of the tubuloglomerular feedback (TGF) in the excretion of salt and water in hydropenic and volume-expanded rats of the spontaneously hypertensive Milan strain (MHS). The rats were 3.5-5 and 5-7 wk old, and age-matched animals from the Milan normotensive strain (MNS) served as controls. There was no difference in mean arterial blood pressure (Pa) between the 3.5- to 5-wk-old prehypertensive MHS (MHSp) and MNS rats, but the glomerular filtration rate (GFR) was higher in MHSp than in MNS [1.35 vs. 0.80 ml X min-1 X g kidney wt (KW)-1, P less than 0.01]. The distal single-nephron glomerular filtration rate (SNGFR) was also higher in MHSp than in MNS (28.6 vs. 20.2 nl X min-1 X g KW-1, P less than 0.05). TGF was determined from both stop-flow pressure response and proximal and distal SNGFR. It was found that MHSp exhibited essentially no TGF response. During development of hypertension 5- to 7-wk-old MHS (MHSd) had a higher Pa than MNS (120 vs. 98 mmHg, P less than 0.01). Normally GFR and SNGFR increase with age, and such was the case with MNS (0.8 to 1.02 ml X min-1 X g KW-1 and 20.2 to 23.4 nl X min-1 X g KW-1), but in MHSd there was a decrease in both GFR and SNGFR with age (1.35 to 1.10 ml X min-1 X g KW-1 and 28.3 to 18.3 nl X min-1 X g KW-1).(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Increased tubuloglomerular feedback activity in Milan hypertensive rats. 371 52

The potential role of atracurium besylate as a trigger or attenuator of the malignant hyperthermia syndrome was tested in six MHS Landrace swine. Animals were tested for susceptibility and then exposed to atracurium given as an i.v. bolus both alone and concomitantly with 2% halothane. In no instance could the syndrome be triggered by atracurium nor did it convincingly attenuate the syndrome when triggered by halothane.
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PMID:The screening of atracurium in MHS swine. 375 46

In earlier work on malignant hyperthermia (MH) susceptible pigs the concentration of muscle metabolites differed from that found in normal control pigs. Therefore, in the present study these metabolites were measured in human muscle biopsies to find out whether normal individuals could be discriminated from MH-susceptible persons. Analysis of skeletal muscle metabolites was performed on skeletal muscle obtained from humans (n = 68) being screened to exclude or confirm susceptibility to MH. Three groups were identified based on the reaction pattern of a skeletal muscle sample exposed in vitro to caffeine or halothane 1% plus caffeine: 1) MH susceptible (MHS; n = 19); 2) normal humans, (controls; n = 31); and 3) intermediate-reaction type (K-type:n = 18). No significant differences were found in metabolite levels of phosphocreatine (normal, MHS, and K-type: 13.20 vs. 13.74 vs. 14.42 nmol/mg wet weight, respectively), creatine (16.30 vs. 16.94 vs. 15.06 nmol/mg wet weight, respectively), adenosine triphospate (3.75 vs. 3.98 vs. 3.89 nmol/mg wet weight, respectively) and lactate (3.73 vs. 3.65 vs. 3.79 nmol/mg wet weight, respectively). It is concluded that analysis of skeletal muscle metabolites cannot be used as a screening test to confirm or exclude MH susceptibility in humans.
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PMID:Comparison of metabolites in skeletal muscle biopsies from normal humans and those susceptible to malignant hyperthermia. 378 37

Malignant haemangioendothelioma of the scalp of the elderly (MHS) is a relatively rate entity. A new case of this uncommon condition, which was subjected to an ultrastructural and immunohistological study, is reported herein.
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PMID:[Malignant hemangioendothelioma of the head in the elderly. Ultrastructural and immunohistological study]. 389 68

The histological and histochemical structure of m. longissimus thoracis, m. semimembranosus, m. triceps brachii-caput longum and m. rectus femoris was compared in pigs with a positive and negative reaction to halothane treatment. As found, the pigs affected by the malignant hyperthermia syndrome have thicker muscle fibres in the studied muscles. In the course of growth, the muscles of these animals have a larger proportion of red fibres (SO), but it is already at the age of 170 days (weight 100 kg) that light fibres prevail. The relative volume of the FG fibres is larger by 1-5%, as compared with the healthy animals. A higher number of pathologically changed fibres occurs in the pigs sensitive to halothane. The histological picture is individually variable; therefore the histological and histochemical methods cannot be considered as objective in view of MHS diagnosis. The general weakening of the constitution of the pigs is discussed as a possible predisposition factor underlying the development of various health disorders.
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PMID:[Muscle fibers of pigs sensitive to halothane and resistant to it]. 392 85

Care of patients with osteoarthritis is compared for three different types of health service: fee-for-service, prepayment (Kaiser Health Plan), and an experimental service (Midpeninsula Health Service--MHS). While charging fees-for-service, MHS modifies medical practice in defined ways and seeks to involve patients through supervised self-care. Volunteer panels of patients provide the data at 6-month intervals through self-administered questionnaires. This report covers the first 18 months. Health status has not changed. Annualized utilization data, adjusted for age, income, education, pain, disability, disease duration, and prescription drug use, indicate that MHS members have fewer physician visits, fewer diagnostic tests, and lower antiinflammatory drug use than the other two services. MHS members express satisfaction equal to or greater than other participants. The results suggest that, in addition to modified financial incentives, there is another strategy that is relevant to achieving effective, efficient, and satisfying health care.
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PMID:Chronic disease and health system performance. Care of osteoarthritis across three health services. 398 7

The proton NMR parameters of suspensions of erythrocytes from normotensive (MNS and WKY strains) and spontaneously hypertensive (MHS and SHR strains) rats have been compared: a marked difference in the relaxation behaviour has been observed for the two classes of strains. Various factors which may give rise to the difference found in dynamic behaviour are discussed.
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PMID:Modified proton relaxation behaviour in erythrocytes from spontaneously hypertensive rats. 631 9


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