Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0024591 (malignant hyperthermia)
 2,353 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Vaginal swabs taken in 211 cases of alleged sexual assault were examined for seminal vesicle-specific antigen (SVSA) using an MHS-5-ELISA (SEMA kit). The results were compared with those obtained by sperm detection by means of light microscopy and the acid phosphatase reaction (ACP), using Phosphatesmo-KM papers. Especially in fresher samples (duration of storage between 10 days and 2 1/2 months), a high degree of correlation was observed between the results of light microscopic and MHS-5 methods. Several cases with positive MHS-5 showed concurrent positive ACP reactions, even though no spermatozoa had been seen microscopically. The results are displayed in the light of time elapsed between the alleged assault and examination of the swabs. The longest time span after the alleged assault in which MHS-5 yielded a positive result was 47 h; in this case spermatozoa were also seen microscopically. SVSA is not totally stable in vaginal swabs stored over long (9 months to 5 1/2 years) periods of time. Furthermore, results in eight penile swabs are reported. MHS-5 is a useful tool for medico-forensic semen detection in vaginal swabs, probably even in cases of azoospermic or aspermia.
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PMID:Evaluation of MHS-5 in detecting seminal fluid in vaginal swabs. 865 22

Objective markers of exposure to semen provide a more valid assessment of condom failure and failure to use condoms than self-reports. The present study evaluated three of the assays commonly used in forensic medicine for detecting semen exposure: acid phosphatase (AP) activity, prostate specific antigen (PSA), and the human seminal plasma antigen (MHS-5). 20 US women were intravaginally inoculated with 6 measured, increasingly larger amounts of their partners' semen. Vaginal fluid was collected with swabs by study participants and tested for the three markers. Before semen inoculation, PSA levels were consistently low (median, 0.11 ng/ml; range, 0-1.25 ng/ml) while those of AP were highly variable (median, 13.4 U/l; range, 0-350 U/l); all preinoculation samples were negative for MHS-5. The median PSA concentration increased consistently with increasing volumes of semen, while median AP and MHS-5 levels showed an inconsistent pattern. All 120 swabs obtained after intravaginal inoculation with semen were positive for PSA, 64 (55%) were positive for AP, and 14 (12%) were positive for MHS-5. These findings indicate that self-sampling of vaginal secretions followed by the PSA immunoassay represents a simple, accurate marker of semen exposure. Because the PSA assay is available in most hospital laboratories for prostate cancer screening, the methodology used in the present study is suitable for widespread application.
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PMID:Objective markers of condom failure. 977 37