Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0024591 (malignant hyperthermia)
 2,353 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Vaginal swabs taken in 211 cases of alleged sexual assault were examined for seminal vesicle-specific antigen (SVSA) using an MHS-5-ELISA (SEMA kit). The results were compared with those obtained by sperm detection by means of light microscopy and the acid phosphatase reaction (ACP), using Phosphatesmo-KM papers. Especially in fresher samples (duration of storage between 10 days and 2 1/2 months), a high degree of correlation was observed between the results of light microscopic and MHS-5 methods. Several cases with positive MHS-5 showed concurrent positive ACP reactions, even though no spermatozoa had been seen microscopically. The results are displayed in the light of time elapsed between the alleged assault and examination of the swabs. The longest time span after the alleged assault in which MHS-5 yielded a positive result was 47 h; in this case spermatozoa were also seen microscopically. SVSA is not totally stable in vaginal swabs stored over long (9 months to 5 1/2 years) periods of time. Furthermore, results in eight penile swabs are reported. MHS-5 is a useful tool for medico-forensic semen detection in vaginal swabs, probably even in cases of azoospermic or aspermia.
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PMID:Evaluation of MHS-5 in detecting seminal fluid in vaginal swabs. 865 22

We isolated DNA from porcine hair roots by silica matrix. Purified DNA is suitable for PCR analysis of malignant hyperthermia in the pig breeds. The using of hair roots as a source of DNA provides noninvasivity and it is also convenient for breeders. We compared amplification of DNA purified by silica matrix and Prep-A-gene kit. DNA purification by silica is as effective as Prep-A-gene purification and it is advantageous because of the low price.
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PMID:A simple and inexpensive DNA purification for malignant hyperthermia PCR detection in porcine hair roots. 2206 27