Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0024591 (malignant hyperthermia)
 2,353 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The location of the antigen recognized by monoclonal antibody MHS-5 in the human reproductive tract was examined by means of enzyme-linked immunosorbant assay (ELISA) and indirect immunohistochemistry employing the strepavidin-biotin-complex method. Homogenates of male reproductive tract tissues and other human organs assayed by ELISA demonstrated immunoreactivity of the MHS-5 monoclonal antibody specifically with human seminal vesicle extracts. Varying ratios of seminal protein and monoclonal antibody ascites were tested to determine the amount of antigen necessary to completely absorb the antibody in the ELISA assay. This ratio was subsequently used to obtain the absorbed negative control for histochemical localization studies. By light microscope examination of seminal vesicle tissue in paraffin section, the MHS-5 antigen was localized in principal cells of the seminal vesicle epithelium. Epididymal sperm, obtained from patients at orchiectomy and vasovasostomy were found to lack the MHS-5 antigen. Following incubation with seminal protein or fluid obtained from the lumen of the human seminal vesicle, epididymal sperm reacted with the MHS-5 antibody on ELISA. These findings indicate that the MHS-5 antigen, a novel protein previously shown to be a unique marker for human semen, is a secretory product of the human seminal vesicle epithelium and may be reconstituted on the surface of epididymal spermatozoa.
Anat Rec 1986 Apr
PMID:Immunohistochemical localization of the MHS-5 antigen in principal cells of human seminal vesicle epithelium. 370 82

Malignant hyperthermia developed in two Landrace x Large White pigs, 75 and 105 minutes after the induction of anaesthesia with halothane. Rapid treatment and discontinuation of halothane anaesthesia were unable to reverse the condition in the first case but were successful in the second. The delayed onset of malignant hyperthermia after delivery of halothane is unusual and for successful treatment careful monitoring and rapid and aggressive therapy are needed.
Vet Rec 1998 Jul 18
PMID:Delayed onset malignant hyperthermia in two pigs. 971 23

Two groups of 21 three-month-old Landrace x Large White pigs were sedated with either azaperone (2 mg/kg), butorphanol (0.2 mg/kg) and ketamine (5 mg/kg) (group A), or detomidine (100 microg/kg), butorphanol (0.2 mg/kg) and ketamine (5 mg/kg) (group D) administered intramuscularly, before being anaesthetised with halothane, oxygen and nitrous oxide for a bilateral stifle arthrotomy. The pigs' heart rate, respiratory rate, mean arterial blood pressure, electrocardiogram, arterial oxygen saturation, arterial blood gases, and oesophageal and rectal temperature were measured while they were anaesthetised and five minutes after they were disconnected, and their recovery times and any complications were recorded. Both groups were well sedated. Their heart rate was unchanged during the period of anaesthesia but increased when they recovered. The respiratory rate, mean arterial blood pressure and rectal temperature were lower in group A than in group D (P<0.05). Mild respiratory acidosis developed during anaesthesia in both groups. Both groups recovered equally rapidly and complications were generally minor, though two pigs in group D appeared to develop malignant hyperthermia.
Vet Rec 1999 Sep 04
PMID:Comparison of two combinations of sedatives before anaesthetising pigs with halothane and nitrous oxide. 1057 38