Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0024530 (
malaria
)
44,886
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The present research reports a detailed in silico analysis of chromatin assembly factor-1 (CAF-1) family in human
malaria
parasite
Plasmodium falciparum
. Our analysis revealed five chromatin assembly factor-1 genes in
P. falciparum
(
Pf
CAF-1) and the
Pf
CAF-1 family was divided into two classes where, Class A belongs to the CAF-1 complex and others are kept in Class B. For comparative studies, orthologs of
Pf
CAF-1 family were identified across 53 eukaryotic species and evolutionary relationships were drawn for different CAF-1 subfamilies. The phylogenetic analysis revealed grouping of evolutionary-related species together, although, divergence was observed in branching pattern. A detailed analysis of domain composition highlighted species-specific features viz
.
species-specific KDDS repeats of 84 amino acids were identified in
Pf
CAF-1A whereas, members of CAF-1C/
RbAp48
and RbAp46 subfamily exhibited least variation in size and domain composition. The qRT-PCR analysis revealed upregulation of
Pf
CAF-1 members in trophozoite or schizont stage. Furthermore, a comparative expression analysis of the available transcriptome and proteome data along with qRT-PCR analysis revealed mixed expression patterns (coordination as well as non-coordination between different studies). Protein-protein interaction network analyses of
Pf
CAF-1 family were carried out highlighting important complexes based on interologs. The
Pf
RbAp48
was found to be highly connected with a total of 108 PPIs followed by
Pf
RbAp46. The results unravel insights into the
Pf
CAF-1 family and identify unique features, thus opening new perspectives for further targeted developments to understand and combat
malaria
menace.
...
PMID:Unraveling CAF-1 family in
Plasmodium falciparum
: comparative genome-wide identification and phylogenetic analysis among eukaryotes, expression profiling and protein-protein interaction studies. 3220 92
