Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0024530 (malaria)
 44,886 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Primary sequence differences have been found between two different ribosomal DNA (rDNA) units of the rodent malaria parasite, Plasmodium berghei, within the coding areas of both the small and large ribosomal RNAs (rRNA). The coding regions of rDNA unit A are protected from nuclease S1 digestion by rRNA isolated from asexual blood stage parasites. Under the same conditions of analysis, the comparable coding regions from unit C are cut into small pieces by nuclease S1, the largest being 1.1 kb. Analysis of heteroduplexes of the respective DNA clones from units A and C by electron microscopy reveals that the two units differ in the 5' flanking and internal transcribed sequences and that there are extensive sequence differences in the DNA coding for the mature large rRNA. No introns were detected in either rDNA unit. The data shows that unit A is transcribed in blood stage parasites and that unit C is not.
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PMID:Two major sequence classes of ribosomal RNA genes in Plasmodium berghei. 637 6

A genes-first approach to genome sequencing is described which efficiently generates gene sequence tags from genomic DNA. Mung bean nuclease (EC 3.1.30.1) cleaves the genomic DNA of many organisms before and after genes and within some introns. Analysis of gene sequence tags prepared from mung bean nuclease-digested Plasmodium falciparum DNA demonstrates that this method has several advantages over the popular cDNA expressed sequence tag approach. To date, 673 sequence tags containing over 215 kb of sequence have been generated from 400 clones. Sixty clones (15%) have significant similarity to sequences in the protein and translated nucleic acid data bases. These represent 51 unique genes, of which only 5 encode previously known P. falciparum proteins. The identified proteins include those expressed in erythrocytic, exoerythrocytic, and gametocytic stages of the parasite. Thirty percent of clones identified appear to carry complete coding regions. The spacer DNA separating genes is rarely cloned. These gene sequence tags will form a useful data base from which to initiate projects to develop new therapeutics, vaccines, and strategies to control human malaria.
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PMID:Gene sequence tags from Plasmodium falciparum genomic DNA fragments prepared by the "genease" activity of mung bean nuclease. 823 27