Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0024141 (systemic lupus erythematosus)
 44,322 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Purified anti-dsDNA antibody was obtained from the serum of patients with systemic lupus erythematosus by affinity-column chromatography. Anti-dsDNA-F(ab')2 fragment (idiotype) was prepared from digested anti-dsDNA antibody with pepsin. We have developed a sensitive and specific method for detection anti-dsDNA-F(ab')2 antibody. Our result revealed that low titer of anti-dsDNA was observed in patients with active stage of systemic lupus erythematosus.
Zhonghua Nei Ke Za Zhi 1992 Jun
PMID:[Detection of anti-dsDNA-F(AB')2 antibody and evaluation of its clinical significance]. 128 85

By using Western blot method, we studied the antihistone fraction antibodies in sera of patients with rheumatic diseases. After analysed with SDS-PAGE, the histone could be separated into H1, H3, H2B, H2A and H4 fractions. The positive rate of anti-histone fraction antibodies was highest (82.1%) in systemic lupus erythematosus (SLE) patients, especially in active stage. In 28 SLE patients, the positive rates of anti-H2B, -H1, -H3, -H2A and -H4 were 78.6%, 60.7%, 50.0%, 35.7% and 7.1% respectively. The results revealed that the determination of anti-histone fraction antibodies was beneficial to the diagnosis of SLE.
Zhonghua Nei Ke Za Zhi 1992 May
PMID:[Study on anti-histone fraction antibodies in patients with rheumatic diseases]. 147 27

The cell surface phenotype of peripheral blood lymphocytes in patients with systemic lupus erythematosus (SLE) was studied with the anti-T4+, T8+ and T4+2H4+ monoclonal antibodies by flow cytometry. 13 patients with active SLE had a markedly low percentage of T4+2H4+ cells but had normal percentage of T8+ cells. Our results reveal that the influence of T cell to B cell is not only because of the quantity of T cells, also because of the function of T cells. The abnormality of T4+2H4+ cells may be important in the pathogenesis of SLE.
Zhonghua Nei Ke Za Zhi 1991 Jul
PMID:[Studies on the T4+2H4+ cell of peripheral blood lymphocyte in systemic lupus erythematosus]. 168 17

The authors reported IL-2 production and T cell subpopulation of peripheral blood lymphocytes from patients with systemic lupus erythematosus(SLE). The results indicated that patients with SLE showed a decreased IL-2 production and a decreased T4/T8 cell ratio. The relation between IL-2 production and T4/T8 ratio was also observed. Our data suggest that IL-2 production correlates significantly with T4/T8 cell ratio.
Zhonghua Nei Ke Za Zhi 1990 Sep
PMID:[The relationship between IL-2 production and T cell subpopulation in patients with systemic lupus erythematosus]. 198 55

Lymphocyte phenotypes of peripheral blood from 32 SLE patients and 30 normal subjects were studied with 13 kinds of monoclonal antibodies using indirect immunofluorescence technique. The results showed that T4+, T8+, T3+ and T11+ cells decreased in active cases of SLE, while Ia+, IL2R1+ and PCA-1+ cells increased, other B-cell phenotypes (B1, B2, B4, IgM, IgG, IgD) showed no significant difference from those of the normal group. Thus, in inactive SLE after therapeutic management, the numbers of T3+, T11+, T4+, Ia+ and IL2R1+ cells no longer show any abnormality, but the numbers of T8+, PCA-1+ cells and the level of serum IgG are still higher than normal. From the above-mentioned results, it was shown that: 1. The key change in active SLE is the abnormality of immunoregulatory T cells, especially T4+ cells; 2. In active SLE, some of the T cells have been activated in vivo and these immunocompetent cells play an important role in immunoglobulin production of B cells; 3. The hyperactivity of humoral immunity is mainly related to the increase of PCA-1+ cells under the regulation of T cells; 4. Both the lymphocyte phenotypes and clinical features of SLE showed heterogeneity.
Zhonghua Nei Ke Za Zhi 1990 Dec
PMID:[Lymphocyte phenotypes and their correlation with clinical features in systemic lupus erythematosus (SLE)]. 198 46

Using immunoblotting technique (IBT) antibodies to Sm, RNP and SSB polypeptides we detected in 173 patients with different rheumatic diseases. It was found that antibodies to Sm polypeptides with molecular weights of 28K(B) and 13.5K(D) could be detected almost only in SLE (34.0% and 30.0% respectively). The positive rates of anti-28K and anti-13.5K polypeptides in SLE detected by IBT were significantly higher than those of anti-Sm by counterimmunoelectrophoresis (12.0%) (P less than 0.05). The antibodies to SSB polypeptides with molecular weights of 48K and 43.41K could be detected mainly in Sjogren's syndrome (70.0% and 65.0% respectively) and the antibodies to RNP polypeptides with molecular weights of 68K, 32K(A) and 29K (B') mainly in MCTD (82.6% 100% and 34.8% respectively) (P less than 0.001). It was reported by Pettersson that anti-68K polypeptide had high specificity for the diagnosis of MCTD. But our data showed that the anti-68K polypeptide could also be detected in SLE(26.0%) and PSS(15.0%) and was not so specific for MCTD.
Zhonghua Nei Ke Za Zhi 1990 Aug
PMID:[The clinical significance of antibodies to SM, RNP and SSR polypeptides detected by immunoblotting technique]. 208 16

The determination of serum AchrA in patients with SLE(43) and other rheumatic diseases (ODRs, 109) and in 42 normal individuals was measured by indirect immunofluorescence assay (IIF) using the polytene chromosomes of the third instar larva of Drosophila melanogaster as the substrate. The results showed that the serum AchrA was negative in all of the 42 normal individuals and the 109 patients with ORDs and the AchrA was positive in 19 (44%) of the patients with SLE. The patients with active SLE were found in 16 (84%) of the 19 AchrA positive group and 13 (54%) of the 24 AchrA negative group. The incidence of arthritis/arthralgia, photosensitivity, glomerulonephritis and anemia in the SLE patients were higher in the AchrA positive group than those in the AchrA negative group. In contrast, the patients with nephrotic syndrome were more common in the AchrA negative group than in the AchrA positive group. It is suggested that serum AchrA is extremely specific and more sensitive for SLE and that the finding of positive AchrA are usually associated with activity of SLE and renal involvement.
Zhonghua Nei Ke Za Zhi 1990 Oct
PMID:[Serum anti-polytene chromosomes of Drosophila melanogaster antibody (AchrA) and systemic lupus erythematosus]. 212 58

Changes of blood coagulation in 32 cases of SLE were investigated. Abnormalities frequently found were elevation of blood fibrinogen, FDP, V111R: Ag levels, prolonged or shortened KPTT time, and depressed AT-III value. Half of the patients with SLE showed laboratory changes compatible with the diagnostic criteria of DIC, but acute DIC was encountered clinically only in 2 cases hypercoagulation state or hypercoagulation with lower fibrinolysis, however were frequently seen. Lupus anticoagulant were detected in 6 patients and deep vein thrombosis of lower extremity in 1 patient. Examination of blood coagulation in patients with SLE was, therefore, of clinical importance.
Zhonghua Nei Ke Za Zhi 1990 Dec
PMID:[Blood coagulation changes in systemic lupus erythematosus]. 212 40

Determination of antineuronal antibodies was carried out by a solid phase radioimmunoassay, in which the neuronal cells SK-N-SH were cultured as the target antigens, the positive rate in sera and CSF of neuropsychiatric SLE patients being 85% and 77.8% respectively, all with quite high level of antibodies. Although 66.7% sera of SLE patients without CNS involvement were also positive for the antibodies, yet the antibody levels of them were distinctly lower, and only 3 of them showed weak positive reactions in their CSF. It was shown that 90% of neuropsychiatric SLE patients with diffuse CNS manifestations had increased antineuronal antibodies, compared with only 20% in cases with local CNS involvements. The antibody levels both in sera and CSF decreased remarkably following the patients' recovery from the neuropsychiatric attack. It is concluded that the antineuronal antibodies might play a role in the pathogenesis of neuropsychiatric lupus and the determination of them in CSF might be useful in the diagnosis and differential diagnosis of neuropsychiatric lupus.
Zhonghua Nei Ke Za Zhi 1990 Mar
PMID:[Antineuronal antibodies and neuropsychiatric systemic lupus erythematosus]. 220 46

Determinations of anti-histone antibodies (AHA) by ELISA were carried out in 109 cases of SLE, 117 of RA, the positive rate being 50.5%, 23.1% respectively, with titres in SLE patients higher than in RA. AHA was 90.2% positive in active cases of SLE patients. SLE patients with AHA showed a higher incidence of pericarditis and arthritis, but a lower rate of malar rash than SLE patients without AHA. In RA, there is a higher incidence of extraarticular manifestations in AHA positive patients IgM-AHA was this predominant AHA in RA while in SLE patients it was the IgG-AHA. For SLE, IgG-AHA which was more closely associated with anti-ds DNA was more significant than IgM-AHA.
Zhonghua Nei Ke Za Zhi 1990 Apr
PMID:[Detection and clinical significance of anti-histone antibody in systemic lupus erythematosus and rheumatoid arthritis]. 222 52


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