Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0024141 (systemic lupus erythematosus)
 44,322 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

An enzyme-linked immunosorbent assay (ELISA) was developed to detect serum antibodies reactive to canine platelets. The method used an anti-canine immunoglobulin G horseradish peroxidase conjugate, O-dianisidine, as a water-soluble chromagen, and pooled platelets as a temporary antibody support. The ELISA was compared with a conventional immunoinjury method measuring the accelerating effect of platelet factor 3 on coagulation. The ELISA was more sensitive in the detection of antiplatelet antibodies in canine patients with idiopathic thrombocytopenic purpura, autoimmune hemolytic anemia, and systemic lupus erythematosus. Serial dilution of rabbit anti-canine platelet serum revealed a positive correlation between the strength of the antiserum and the degree of color measured spectophotometrically, indicating that the absorbance value may be useful in estimating the amount of antibody present in the serum in indirect tests and on the platelets in direct tests.
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PMID:Application of the enzyme-linked immunosorbent assay for the detection of platelet antibodies in dogs. 654 3

A 10-year-old girl with systemic lupus erythematosus (SLE) and autoimmune hemolytic anemia developed acute leukemia 10 months after the diagnosis of SLE. Leukemic cells had both the myeloid character, as shown by peroxidase activity, and T-cell surface antigen. The SLE-like syndrome as a paraneoplastic syndrome is discussed.
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PMID:Acute myeloid leukemia with preceding systemic lupus erythematosus and autoimmune hemolytic anemia. 658 57

The inflammatory infiltrates of cutaneous lupus lesions from 14 patients with benign cutaneous, subacute cutaneous or systemic lupus erythematosus were examined for T and B lymphocytes, macrophages and Ia positive cells using monoclonal antibodies and a peroxidase antiperoxidase technique. T cells and Ia positive cells were present in abundance followed by B cells then macrophages. Approximately equal numbers of helper/inducer T cells (OKT4, Leu3a) and suppressor/cytotoxic T cells (OKT8, Leu-2a) were present in the inflammatory infiltrate.
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PMID:Characterization of the inflammatory infiltrate in lupus erythematosus lesions using monoclonal antibodies. 660 42

This article gives a synopsis of the inflammatory reactions as well as its mediators under special consideration of the efferent part of the reaction. There is no doubt that histamine, complement, and the kinin system play an essential role; arachidonic acid (eicosatetraenic acid) and its metabolites, however, have gained comparable significance: prostaglandines, prostacyclines, and thromboxanes as metabolites of the cyclo-oxygenase, the leucotrienes SRS-A (slow reacting substances of anaphylaxis) and ECF (eosinophilic chemotactic factor) mediated via lipoxygenase. Moreover, oxygen and its metabolites hydrogen peroxide (H2O2), peroxide radicals (O-2), and hydroxyl radicals (.OH) as well as activated oxygen (singulett oxygen (1O2) play an important part with all aerobic living organisms. Inborn enzyme deficiency of the oxygen metabolism such as NADPH oxidase or cytochrome b-245 deficiency lead to chronic septic granulomatosis. The disease is characterized by reduced resistence against infections, decreased phagocytosis, insufficient killing of bacteria by leucocytes, and diminished oxygen burst. Thus the underlying enzyme deficiency leads to reduced formation of peroxide radicals frequently causing infections with septic complications. On the other hand, increased formation or reduced degradation of peroxide radicals may result in pathological reactions like chromosomal alterations, lipidperoxidation or oxidation of sulph-hydryl groups. The fact that increased peroxide radical formation may cause inflammation or chromosomal aberration is of importance with regard to the pathogenesis of several chronic inflammatory diseases of unknown etiology, such as systemic scleroderma or lupus erythematodes. The enzyme superoxide dismutase (SOD) converts peroxide radicals (O-2) into hydrogen peroxide (H2O2) which can be inactivated by catalase or peroxidase. Consequently, treatment with SOD may have an effective influence on chronic inflammatory dermatoses of unknown pathogenesis.
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PMID:[Biochemical aspects of the inflammatory reaction - with special reference to oxygen]. 666 95

Twenty-two haematological parameters were measured in 30 patients with systemic lupus erythematosus (SLE) at 110 patient attendances. Using a 10,000 cells per sample automated differential counter, the major abnormalities demonstrated were: lymphopenia, monocytopenia, eosinophilopenia (each of which showed strong correlation with steroid therapy) and increased numbers of cells of high peroxidase activity. Despite the common lymphocytopenia elevation of large unstained cells was noted in 20% of patients. However, no patient with severe disease had a lymphocyte count above 1.9 X 10(9)/l or a haemoglobin above 11.7 g/dl.
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PMID:Haematological aspects of systemic lupus erythematosus: a reappraisal using automated methods. 681 Jun 23

A micro-ELISA technique has been developed to measure antibodies to native DNA and used in SLE patients. The distribution of antibody to native DNA in the main immunoglobulin classes was studied, using anti-human globulin conjugates labelled with peroxidase. the antigen (double-stranded DNA from calf thymus) used in the assay was adsorbed to the surface of polystyrene plates treated with methylated bovine serum albumin. The standardization of the method was carried out by use of globulin calibration curves.
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PMID:Enzyme-linked immunosorbent assay for antibodies to native DNA in sera of patients with SLE. 697 97

A solid-phase assay for the detection of anti-F(ab')2 antibodies is described. Wells of microtiter plates are coated with F(ab')2, dilutions of sera are added, and IgG bound to the solid phase is detected using peroxidase-labeled Protein A. Anti-F(ab')2 antibodies were found in 61% of sera from patients with rheumatoid arthritis, 77% with subacute bacterial endocarditis , and 34% with systemic lupus erythematosus. Simultaneous analysis of these sera for immune complexes (IC), using modified Clq and monoclonal rheumatoid factor assays, showed that a correlation existed between anti-F(ab')2 antibodies and the levels of IC. Characterization of anti-F(ab')2 antibodies by inhibition and absorption experiments and by immunological and physical means indicated that they were similar to serum proteins described in the 1960s and designated pepsin agglutinators.
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PMID:Association of anti-F (ab')2 antibodies (pepsin agglutinators) with immune complexes as determined by enzyme-linked immunosorbent assays. 697 96

Experiments were undertaken to determine if porcine C1q could replace human C1q in the solid-phase immunoassay of human immune complexes (ICs). Porcine C1q was obtained by a two-cycle precipitation method involving dialysis against chelating agents in low ionic strength buffer. C1q was adsorbed to polystyrene beads and in vivo- or in vitro-formed ICs binding to the solid-phase C1q were detected with 125I-labeled or horseradish peroxidase-conjugated anti-human gamma antibodies. Unfractionated, heat-aggregated human gamma globulin (delta IgG) could be detected at 20 ng/ml when diluted in buffer only. The detection threshold changed to 40-80 ng delta IgG/ml when the assay was run with buffer containing normal human serum diluted 1: 1000 (the serum dilution used for detecting natural ICs). Analysis of systemic lupus erythematosus sera revealed that 60% contained highly significant levels of ICs (binding greater than or equal to 3 S.D. above the mean of controls). Comparison with platelet aggregation test results revealed a highly significant correlation between the two methods (P less than 0.0001), even though each assay detected ICs in several serum specimens negative in the other test. These results demonstrate that porcine C1q can functionally replace human C1q in the solid-phase immunoassay of human ICs. Since porcine blood is normally a waste product of the meat-processing industry, it is an obvious source of easily isolated C1q for use in such an assay.
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PMID:Porcine C1q and the solid-phase immunoassay of human immune complexes. 697 62

Protein A from Staphylococcus aureus, a cell wall protein with high affinity binding properties for IgG-type antibodies, has been labeled with peroxidase to form a stable immunohistological tracer molecule of relatively low molecular weight. It has been used for demonstration and titration of antinuclear antibodies in SLE sera on mouse liver sections in an indirect technique. The findings were consistent with those obtained by immunofluorescence and by staining with peroxidase-coupled anti-IgG. In contrast to immunofluorescence, the stained sections could be mounted and stored for documentation. In comparison, unspecific tissue adsorption and staining could be minimized by addition of glucose, galactose, and mannose as well as bovine serum albumine to the buffer containing Protein A-peroxidase.
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PMID:A new immunoenzyme tracer for immunohistology: peroxidase-labeled protein A. Its application for determination of antinuclear antibodies. 699 81

Using a specific substrate, no leucocyte elastase activity could be detected in 55 synovial fluids, including 29 from patients with rheumatoid arthritis (RA). However, a high percentage of samples contained phagocytic inclusions of elastase, alpha 1-proteinase inhibitor (alpha 1-PI) and alpha 2-macroglobulin (alpha 2-MG) in both the polymorphonuclear (PMN) and mononuclear phagocytes. Immunofluorescence and indirect peroxidase-antiperoxidase staining of articular cartilage (ACA) from 52% of 21 patients with RA and one with juvenile RA (JRA) showed presence of elastase in the superficial layer of microscopically intact but proteoglycan depleted pannus-free ACA. In histologically altered pannus-free RA-ACA superficial elastase deposits were found in 24% of the cases. Adjacent ACA sections contained IgG, C3, alpha 1-PI and rarely alpha 2-MG. RA-ACA below or surrounded by pannus showed close contact with intact and decaying PMN in 62% and 48% of the cases, respectively. ACA specimens from patients with degenerative disease and systemic lupus were negative. These findings strongly suggest that PMN leucocyte elastase is operative in the degradation of RA-ACA and JRA-ACA, and that this activity is largely dependent upon the presence of entrapped immune complexes in such cartilage.
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PMID:Degradation in vivo of articular cartilage in rheumatoid arthritis by leucocyte elastase from polymorphonuclear leucocytes. 705 Dec 54


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