Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0024141 (systemic lupus erythematosus)
 44,322 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Activated charcoal with various coating materials were screened and tested as adsorbents in hemoperfusion. Among them silicon rubber polyurethane, crosslinked agrose, polyvinyl acetate, polyhydroxy ethyl methacrylate showed good blood compatible properties. Various types of resins and carbonized resins with different functional groups, and structures were tested for the adsorption of small molecules i.e. creatinine, barbiturates, middle molecules i.e. VitB12, inulin, cytochrome C and large molecules i.e. unconjugated bilirubin. High adsorption capacities were obtained and uncoated carbonized resins showed satisfactory blood compatible properties. NK-107 a macroporous, non-coated resin is now being manufactured and successfully used clinically on patients for acute detoxification of hypnotic drugs. Polysaccharide dialdehyde and polystyrene derivatives etc. were used for the removal of urea, ammonia, potassium and phosphorus. DNA immune adsorbents were prepared for systemic lupus erythematosus therapy.
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PMID:Advances of adsorbents for hemoperfusion in China. 228 12

Isolated submandibular gland calcifications were found in a young woman with systemic lupus erythematosus (SLE) undergoing intermittent hemodialysis for 2 years. The calcifications appeared to be due to multiple factors including high calcium-phosphorus product and possibly local inflammation due to SLE.
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PMID:Reversible submandibular gland calcifications in a hemodialyzed woman with systemic lupus erythematosus. 365 65

A microchemical assay for phosphorus was applied to the measurement of DNA in immune complexes formed with monoclonal or serum anti-DNA autoantibodies and DNA of varying size and conformation. Two monoclonal antibodies were produced by hybridomas derived from spleen cells of autoimmune MRL-lpr/lpr mice and were purified from culture fluid by affinity chromatography on columns of goat anti-mouse Ig-Sepharose. Double-helical DNA fragments were prepared by brief digestion of calf thymus DNA with micrococcal and S1 nucleases and fractionation on Sepharose 4B; their double-stranded structures was confirmed by measurement of thermal denaturation. Immune complexes were formed with monoclonal or serum antibodies and native DNA or DNA fragments or denatured DNA; the complexes were precipitated with goat anti-mouse IgG and washed, and DNA phosphorus content of the precipitates was measured. With one monoclonal autoantibody (H241), there were discontinuous increases in the amount of DNA that could be bound (and decreases in the antigen concn required for half-maximal binding) as the DNA size increased. There were especially marked increases in binding efficiency as fragment size increased from an average of 100 (range 85-105) to an average of 150 (range 105-170) base pairs, and again between 450 (range 360-620) and 600 (range 425-825) base pairs. A second monoclonal antibody (H143) did not show significant variation in binding with DNA fragments larger than 300 base pairs. With smaller fragments, the amount of DNA bound by H143 was reduced, but the DNA concn required for half-maximal binding was not. Affinities of these monoclonal antibodies were within the spectrum of human systemic lupus erythematosus serum IgG anti-DNA autoantibodies. The dependence of binding on mol. wt is important in the evaluation of these monoclonal antibodies as biochemical reagents and as potential participants in formation of immune complexes in vivo.
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PMID:Binding of monoclonal anti-native DNA autoantibodies to DNA of varying size and conformation. 387 30

137 patients on maintenance dialysis were studied. All but 2 patients were ambulatory, and all patients demonstrated good to normal strength on manual motor tests. With the exception of systemic lupus erythematosus, no correlation was found between primary diagnosis and patients' fatigue ratings. Laboratory studies of hematocrit, BUN, creatinine, calcium, and phosphorus did not correlate with fatigue ratings for the majority of patients. Fatigue appeared more problematic for patients who had been dialyzing for less than 4 years. Depression was pronounced among patients who reported feeling fatigued upon arising.
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PMID:The problem of fatigue in dialysis patients. 711 Apr 64

Anticardiolipin (aCL) and anti-beta 2-glycoprotein I(anti beta 2GPI) antibodies have been shown in animal models as not cross-reacting antibody populations. This observation prompted us to prove if anti-beta 2GPI exist in human sera by using a reliable method and then to investigate if these are independent from aCl antibodies. We have developed a new ELISA for the detection of anti-beta 2GPI antibodies employing the coating of the protein in carbonate buffer to irradiated microtitre plates and the filtration of serum samples, that makes irrelevant the binding to the uncoated wells. IgG F(ab)2 fragments from IgG positive sera were shown bind beta 2GPI, providing that the binding was a specific antibody binding, mediated by the antigen binding site of the antibody molecule: moreover the antibodies were not able to differentiate native and delipidated beta 2GPI coated plates, making a possible role of a phospholipid contaminant unlikely. On the other hand, the phosphorus content of native as well as delipitated beta 2GPI was undetectable. IgG, but not IgM, anti-beta 2GPI antibodies were classically inhibited by the addition of soluble beta 2GPI, while cardiolipin liposomes appear to modify the reaction in a completely different way, possibly by the described interaction between cardiolipin and beta 2GPI.(ABSTRACT TRUNCATED AT 250 WORDS)
Lupus 1995 Apr
PMID:Anti-beta 2-glycoprotein I antibodies: a marker of antiphospholipid syndrome? 779 15

Preparative thin-layer chromatograms of chloroform-methanol extracts of Borrelia burgdorferi (B31) sonicates showed four fractions (Rf values of 0.84, 0.81, 0.66 and 0.61) that stained with iodine vapors, orcinol, or phospray, suggesting the presence of lipid-, carbohydrate-, and phosphorus-containing compounds. Sera from patients with Lyme disease showed IgM or IgG antibody reactivity to hydrophobic fractions, designated F1 and F2, in both early and late stages of the disease. Lack of constitutive amino acids in these fractions was shown by protein, amino acid, and peptide detection analyses. Sera from patients with syphilis, systemic lupus erythematosus, and antiphospholipid syndrome reacted to one or both of the fractions. Adsorption of sera from Lyme disease patients with intact B. burgdorferi resulted in significantly different pre- and postadsorption patterns of reactivity by whole cell ELISA, whereas adsorption with F1 and F2 resulted in similar pre- and postadsorption patterns. These fractions may not be present in aqueous whole cell or whole cell lysate ELISA antigens or in immunoblots.
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PMID:Nonprotein antigens of Borrelia burgdorferi. 844 Sep 37

The recognition of DNA-like phosphorylated polymers by anti-DNA antibodies from the plasma of systemic lupus erythematosus patients was evidenced a few years ago by our research group. However, the radioimmunological Farr assay used for the assessment of anti-DNA antibodies adsorption was not sensitive enough to give accurate results, particularly in the case of weak levels of antibodies. An alternative method based on the use of radiolabelled species was set up in order to check the validity of previous results. Polystyrene resins with different levels in phosphate groups substitution were assessed with regard to their interactions with anti-DNA antibodies. Results show that the anti-DNA antibodies affinity is dependent on the composition of the polymers and reaches a maximum for a composition of 17.5-22.5 mol of phosphorus per 100 mol of monomeric units. This composition corresponds to the DNA-like polymer previously described. A computer-assisted method was used in order to have an insight into the structure of the active sites responsible for the DNA-like behaviour of this polymer. Numerical simulations of the phosphorylation reaction were performed using a Monte Carlo method, taking the structure predictions and the environment of the phosphorylated units into account. A number of thus generated virtual polymers correlated with the experimental results of the adsorption of anti-DNA antibodies. The chemical structure of the active site was determined by computations introducing selected hypotheses on the structure of the phosphorylated units. Moreover, since the number of active sites is directly related to the number of adsorbed anti-DNA antibodies in the experimental results, the most probable structure of the active sites is proposed and compared to a fragment of DNA. Conclusions are that the distances between the phosphate groups in the active sites of the DNA-like polymer and in the DNA fragment are similar. Optimal conditions for the purification of SLE sera by highly specific liquid chromatography using phosphorylated polystyrene resins of precise compositions as stationary phases can thus be envisaged, as well as a new method for the detection of anti-DNA antibodies.
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PMID:Biospecific polymers: recognition of phosphorylated polystyrene derivatives by anti-DNA antibodies. 919 32

In this report, the outcome, diagnosis, management, and complications of pregnancy in dialysis patients are discussed. The advantages and disadvantages to the use of peritoneal dialysis and hemodialysis and the changes in dialysis regimen used in pregnant women are addressed. Maternal complications, particularly hypertension and anemia, are reviewed. This report looks at the approach to the management of anemia and calcium/phosphorus metabolism in the setting of limited information. The report also discusses pregnancy outcome for the mother and fetus, including the problem of prematurity and fetal loss. Special considerations in women with lupus and diabetes are noted. Pregnancy in dialysis patients remains a high-risk undertaking for both the patient and the infant. There are large gaps in our knowledge base regarding the effect of the abnormalities associated with renal failure on pregnancy. The survival of the infant and the safety of the mother depend on close cooperation among all the specialities involved, including nurses, doctors, nutritionists, and social workers from nephrology, perinatology, and neonatology.
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PMID:Management of the pregnant dialysis patient. 947 12

The aim of this study was to assess the skeletal metabolism in a murine model of systemic lupus erythematosus (SLE). MRL/n and MRL/l mice (respectively representing a benign and a malignant form of the disease) were observed from 1.5 to 6.5 months of life. The monthly follow-up included: biochemical and histomorphometrical studies of the femoral bone, serum biochemistry, immunoglobulins and osteocalcin, and histological evaluation of the kidney tissue. The results showed a higher femoral weight (+11.5%), calcium (+4.4%) and protein bone content (+11.4%) and a significantly higher (+77%) phosphorus bone content in the MRL/n group; significantly lower (-48.9%) bone alkaline phosphatase enzymatic activity, lower bone alkaline/acid phosphatase enzymatic activities ratio (-40.8%) and lower (-38.4%) serum osteocalcin values in the MRL/l group (which might suggest reduced bone formation in these animals); markedly smaller trabecular bone volume (BV/TV) in the femoral head (-36.2%) and femoral neck (-39.8%), and smaller cortical and femoral areas in the mid-femoral shaft (-38.8% and -38.1% respectively) in the MRL/l group; higher serum immunoglobulins, increased serum blood urea nitrogen (BUN) and creatinine and a higher index of activity in the kidney histology in the MRL/l group, indicating increased activity of the disease in this substrain. The MRL mice, through their two substrains, may serve as a valuable laboratory animal model for study of the skeletal changes in SLE and of the influence of the disease activity on the skeletal metabolism.
Lupus 2001
PMID:Osteoporosis in murine systemic lupus erythematosus--a laboratory model. 1143 79

Beyond its effects on bone metabolism, calcium and phosphorus homeostasis, 1,25-dihydroxyvitamin D3 (1,25(OH)(2)D(3), calcitriol) exerts profound effects on the immune system. We here provide an overview over the metabolism, molecular and cellular action of 1,25(OH)(2)D(3) with particular regard to its immunomodulatory function. Effects of 1,25(OH)(2)D(3) on the immune system are manyfold and include suppression of T cell activation, shaping of cytokine secretion patterns, induction of regulatory T cells, modulation of proliferation, and interference with apoptosis. 1,25(OH)(2)D(3) further influences maturation, differentiation, and migration of antigen presenting cells. Altogether, its immunomodulatory potency is comparable to other established immunosuppressants without sharing their typical adverse effects. This profile makes 1,25(OH)(2)D(3) a potential drug for the treatment of immune-mediated diseases. Yet, the major obstacle for its clinical use, its potent calcemic activity, is not overcome to date. The identification or generation of novel vitamin D derivatives with dissociated calcemic and immunomodulatory properties is therefore a major task. Its success might eventually lead to promising drugs for future therapeutic exploitation of a wide array of immune diseases, such as psoriasis, multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus, and others.
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PMID:Immunoregulation through 1,25-dihydroxyvitamin D3 and its analogs. 1558 87


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