Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0024141 (systemic lupus erythematosus)
 44,322 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Three human T-cell clones with activated killer activity (5B5, 5C1, and 7B5) which could lyse various tumor cell lines were established. The cytotoxic activity of these clones was decreased by incubation with anti-CD3 monoclonal antibody, suggesting that they recognized tumor cells by T-cell antigen receptor. A monoclonal antibody which blocked the cytotoxic activity of clone 5B5 was obtained. This antibody (N1977) blocked the binding and cytotoxic activity of clone 5B5 at the target cell level, suggesting that the antigen defined by N1977 antibody, designated as ATM-1, was a target molecule recognized by 5B5 cells. ATM-1 in the conditioned medium of a cancer cell line (NBT-2) and serum from a patient with lung cancer was characterized by following its immunoreactivity. On gel filtration, both the conditioned medium and the serum gave three peaks of ATM-1 immunoreactivity, corresponding to approximate molecular weights of 1,200,000, 700,000, and 120,000, respectively. They were chromatofocused at pH 4.0, 4.8, and 6.5, respectively. The high molecular weight forms were shown to be molecules with the disulfide-linked elementary glycoprotein with ATM-1 immunoreactivity and approximate molecular weight of 120,000. Most of the molecules with ATM-1 immunoreactivity bound to both concanavalin A and wheat germ agglutinin, and their binding activity to the antibodies was lost by treatment at 60 degrees C for 30 min. An assay of ATM-1 level in sera was performed by a sandwich enzyme immunoassay. The following positive percentages were obtained from preliminary clinical studies: breast cancer, 67% (8 of 12 cases); hepatocellular carcinoma, 83% (10 of 12 cases); gastric cancer, 58% (7 of 12 cases); lung cancer, 41% (5 of 12 cases); hematological malignancies, 0% (0 of 9 cases); systemic lupus erythematosus, 0% (0 of 8 cases); rheumatoid arthritis, 0% (0 of 8 cases).
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PMID:Identification of a tumor-associated target antigen, ATM-1, for a human T-cell clone with activated killer activity and its existence in sera of cancer patients. 304 79

The article presents results of examination designed to study parameters characterizing the peripheral blood neutrophils functional activity in leukocytopenia in patients with diffusive disorders of the connective tissue (systemic lupus erythematosus and rheumatoid arthritis), with the above values having come to be used in the assessment of severity of the course, differential diagnosis of complications, and prognostication of the condition. Such cytochemical indices as activity of myeloperoxidase, amounts of cationic proteins, capability of neutrophils to reduce nitroblue tetrazolium to diphormazine (NBT-test) are considered to be of much informative value which have come to be widely used in the diagnosis and prognosis of a great many of various medical problems. Defects in neutrophils are shown, disturbances are pointed out in their functional activity evidenced by the decline in their capability of reducing NBT. Poststimulation metabolic activity is but inadequately increased as compared to results in the control group. Of practical value is screening of patients presenting with low values for the functional reserve of heterophilic leucocytes, the purpose of which screening being early prognostication of the course of pyo-inflammatory complications.
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PMID:[Clinical patameters associated with oxygen-dependent metabolism in neutrophils in patients with systemic lupus erythematosus and leukocytopenia]. 1145 12