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Target Concepts:
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Query: UMLS:C0024141 (
systemic lupus erythematosus
)
44,322
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Hemorrhagic myocarditis is a potentially fatal complication of excessive levels of systemic inflammation. It has been reported in viral infection, but is also possible in systemic autoimmunity. Epicutaneous treatment of mice with the Toll-like receptor 7 (TLR-7) agonist Resiquimod induces auto-antibodies and systemic tissue damage, including in the heart, and is used as an inducible mouse model of
systemic lupus erythematosus
(
SLE
). Here, we show that overactivation of the TLR-7 pathway of viral recognition by Resiquimod treatment of
CFN
mice induces severe thrombocytopenia and internal bleeding, which manifests most prominently as hemorrhagic myocarditis. We optimized a cardiac magnetic resonance (CMR) tissue mapping approach for the
in vivo
detection of diffuse infiltration, fibrosis and hemorrhages using a combination of T
1
, T
2
and T
2
*
relaxation times, and compared results with
ex vivo
histopathology of cardiac sections corresponding to CMR tissue maps. This allowed detailed correlation between
in vivo
CMR parameters and
ex vivo
histopathology, and confirmed the need to include T
2
*
measurements to detect tissue iron for accurate interpretation of pathology associated with CMR parameter changes. In summary, we provide detailed histological and
in vivo
imaging-based characterization of acute hemorrhagic myocarditis as an acute cardiac complication in the mouse model of Resiquimod-induced
SLE
, and a refined CMR protocol to allow non-invasive longitudinal
in vivo
studies of heart involvement in acute inflammation. We propose that adding T
2
*
mapping to CMR protocols for myocarditis diagnosis improves diagnostic sensitivity and interpretation of disease mechanisms.This article has an associated First Person interview with the first author of the paper.
...
PMID:Characterization of acute TLR-7 agonist-induced hemorrhagic myocarditis in mice by multiparametric quantitative cardiac magnetic resonance imaging. 3132 89
