Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0024141 (systemic lupus erythematosus)
 44,322 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A beaded pattern of immunofluorescence (IF) occasionally occurs in routine FTA-ABS testing. This phenomenon strongly correlates with false-positive reactions in patients with SLE. We report on this IF finding in association with carcinoma of the colon.
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PMID:Beaded fluorescence pattern of FTA-ABS test associated with malignancy. 37 36

The objective of this study was to investigate the false-positive reactions in the Kline, FTA200 and FTA-ABS tests in acute disseminated lupus erythematosus, polyarthritis and controls. Though the false-positive Kline tests are frequent in autoimmune diseases, false-positive FTA200 when made on non lyophilised treponema are uncommon. On the other hand, when lyophilised treponema are used as substrate for the immunofluorescence, false-positive FTA200 and FTA-ABS are more common in the patients with autoimmune diseases than in controls. The false-positives had two patterns of fluorescence "beaded" or "homogeneous". The present investigation suggests that the false-positive reactions of the beaded type are due to the presence of anti-DNA antibodies in the sera.
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PMID:Laboratoire d'Hygi'ene de la Facult'e de M'edecine de Lyon Universit'e Claude-Bernard, Domaine Rockefeller, 8, avenue Rockefeller, 69373 Lyon Cedex 2 France. 78 44

Sera of patients with lupus erythematosus can produce false-positive reactions in most serologic tests for syphilis, including the FTA-ABS test. False-positive reactions in the FTA-ABS test can exhibit beaded, borderline or reactive patterns of fluorescence. Beaded fluorescence is commonly associated with anti-DNA antibody and other correlates of lupus activity. Borderline and reactive results are common in both systemic and discoid lupus erythematosus, and are usually not associated with increased clinical activity. TPI and MHA-TP tests appear helpful in detecting false-positive FTA-ABS results.
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PMID:Lupus erythematosus and reactive tests for syphilis: update. 101 53

The Venereal Disease Research Laboratory (VDRL) test for syphilis was used to screen 19,067 Jamaican sera, and positive were confirmed with the fluorescent Treponema pallidum haemagglutination assay (FTA-ABS). The group included 15,876 persons applying for emigration, 3039 pregnant women attending prenatal care, and 152 patients with systemic lupus erythematosus (SLE). 2.8% of the general population were VDRL positive, and of these 21.3% had titers 1:4. 4.7% of the pregnant women were positive, 48.2% with titers 1:4. 27.1% of the VDRL positive in the general population and 30.9% of the pregnant women were false positive, i.e.negative on the FTA-ABS test. 78.2% of the SLE patients were false positive on the VDRL, including 1 patient with a titer of 1:128. Thus the percentage of false positive taking the whole group as denominator was 0.59% in the general population, 0.72% in the pregnant women, but 11.8% among SLE patients. The overall seroprevalence of syphilis was 2.2%. These results are atypical in that pregnant women are usually considered to have a high rate of false positive results on the VDRL.
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PMID:Biological false positive serological tests for syphilis in the Jamaican population. 218 94

A 41-year old male who presented for blood donation for the first time was rejected on the grounds of a positive reaginic serological test (VDRL and RPR) and a specific antitrepomena test (FTA) for syphilis. Later studies revealed the existence of a lupus anticoagulant and this was made responsible for the positive serology. Our observation suggests the need to search for the existence of a lupus anticoagulant in blood donors with non-specific or specific positive antitrepenema test.
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PMID:Lupus anticoagulant and false positive serological tests for syphilis. 311 73

Serum samples of 78 patients with systemic lupus erythematosus, systemic sclerosis and other immunological diseases were tested for antibodies to syphilis. Reactive or weak reactive results were observed in 10% by means of the treponema pallidum hemagglutination (TPHA) test, in 27% by the FTA-Abs test, in 40% applying the IgM-FTA-Abs test, in 10% by the VDRL test and in 3% of the cases using the cardiolipin CF test. Only in 3 patients (4%) we found an antibody pattern characteristic of syphilitic patients (TPHA and FTA-Abs test simultaneously undoubtedly reactive). Neither the comparative qualitative and quantitative determination of antibodies to ANA, nDNS and ENA (extractable salinesoluble nuclear antigen) nor elimination of nDNS and ENA antibodies, or incubation of the treponemal test antigen with DNase lead to a conclusion whether the reactive results of the TPHA, FTA-Abs, and IgM-FTA-Abs tests specifically indicate a syphilitic infection. The low incidence of reactive syphilis tests in SLE and the presence of syphilitic antibodies in other immunological diseases limitate the significance for the criterium in the diagnosis of SLE.
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PMID:[Incidence and diagnostic significance of reactive syphilis antibodies in systemic lupus erythematodes and other immunologic diseases]. 637 75

Two hundred forty-five sera submitted to the Center for Disease Control, Atlanta, Ga., (CDC) were analyzed serologically in an attempt to demonstrate the diagnostic value of the Treponema pallidum immobilization (TPI) test when performed in addition to the fluorescent treponemal antibody-absorption (FTA-Abs) test. Diagnoses for the patients whose sera were tested were based on information supplied by the referring physicians. Fifty-four per cent of the diagnostic problems were resolved merely by the finding of a negative result with the FTA-Abs test. There was agreement between the serologic results of the referring laboratory and those of the CDC for 76% of sera tested by the Venereal Disease Research Laboratory test and for 71% of sera tested by the FTA-Abs test. For patients with treponemal disease, the sensitivity of the TPI test was 56% and that of the FTA-Abs test was 78%. When the FTA-Abs test was reactive, a negative TPI test was not significantly associated with systemic lupus erythematosus (P > 0.6) or other collagen vascular disease (P > 0.6), nor was a positive TPI test associated with treponemal disease (P > 0.09). It is concluded that once the result of the FTA-Abs test is known, the TPI test does not provide additional diagnostic information.
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PMID:Failure of the Treponema pallidum immobilization test to provide additional diagnostic information about contemporary problem sera. 700 73

Four hundred and forty-nine syphilis sera, positive in VDRL and FTA-ABS tests, were studied for the presence of circulating immune complexes (CIC) by means of anti-antibody (AA) neutralization test. Ninety-three (20.7%) sera had demonstrable CIC. Subsequently, dispersion of CIC by saline suspension of cardiolipin and by extract of rabbit testicular syphiloma was examined. Of 40 CIC-containing sera tested with cardiolipin, dispersion of CIC was noted in 16 instances; of 32 CIC-containing sera examined with syphiloma extract, dispersion was noted in 16 cases. Significantly, CIC that showed definite dispersion by cardiolipin were not dispersed by syphiloma extract and vice versa. Some studies were also performed on lepromatous leprosy and SLE. None of 27 cIC-containing lepromatous leprosy sera were affected by cardiolipin, but in 2 of 9 CIC-containing SLE sera, cardiolipin dispersed the complexes. Cardiolipin-anti-cardiolipin complexes that neutralized AA were frequently observed when cardiolipin was added to sera containing its corresponding antibodies but not CIC. Similar reactions were noted between the syphiloma extract and anti-treponemal antibodies.
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PMID:Immune complexes in syphilis sera. 719 25

In the New Zealand black (NZB) x New Zealand white (NZW) (B/W) mouse model of systemic lupus erythematosus (SLE), females die prematurely and males have late onset of disease. It has been proposed that testosterone protects B/W mice from rapidly progressive SLE. The mechanisms of androgenic suppression of the immune system, however, are not understood. We tested the hypothesis that testosterone exerts protective effects in males through classic receptor-mediated actions. Flutamide, a potent and specific androgen receptor blocker, was administered continuously to B/W mice from 6 weeks of age, and the animals were monitored in a longevity study. Our supposition that flutamide treatment would accelerate disease was upheld in female B/W mice. In females, flutamide clearly accelerated mortality and reduced longevity to (median) 30 weeks as compared with 37 weeks in controls (p = 0.003). In male B/W mice, mortality was identical in treated and control animals in the early phase of the study, and deleterious effects of treatment were apparent only after the first year of treatment. Flutamide-treated males had significant elevation of serum testosterone, but their atrophied seminal vesicles strongly suggested that peripheral androgen effects were blocked. Albuminuria, blood urea nitrogen, and anti-DNA antibodies were not altered by flutamide in females or males. The receptor-mediated effects of androgens on murine autoimmune disease were dependent on gender and were noted primarily in females and in males that survived past 1 year of age.
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PMID:Accelerated deaths from systemic lupus erythematosus in NZB x NZW F1 mice treated with the testosterone-blocking drug flutamide. 808 83

Enzyme-linked immunoabsorbent assay (ELISA) techniques for the detection of antibodies to the Venereal Disease Research Laboratories (VDRL) antigen as well as for the estimation of antibodies to cardiolipin (aCL) in cerebrospinal fluid (CSF) were performed in several groups of patients, including those with definite paretic neurosyphilis (DPNS, 10 patients), probable paretic neurosyphilis (PPNS, 19 patients), systemic lupus erythematosus (SLE, 71 patients), and miscellaneous neurologic disorders (30 patients), and normal subjects (11 patients). In the DPNS group, all demonstrated positive VDRL by ELISA, and 7 also had positive aCL tests, whereas only 7 of the 10 had positive CSF fluorescent treponemal antibody absorption (FTA-ABS) and Treponema pallidum hemagglutination assay (TPH A) tests. Three had positive VDRL tests by flocculation. In the PPNS group, no patients had positive FTA-ABS, TPH A, or VDRL flocculation tests in CSF. However, 18 of the 19 had positive CSF VDRL by ELISA; two of these also had positive aCL tests. Four SLE patients had positive CSF aCL tests (three with positive ELISA VDRL). Only one patient in the miscellaneous group had positive CSF aCL (Guillain-Barre syndrome), and one had positive IgM VDRL tests (bacterial meningitis). The value of VDRL ELISA in the diagnosis of neurosyphilis in the face of other negative conventional tests is established by our study and had important therapeutic consequences in patients with possible/probable neurosyphilis. The aCL test often may be positive in patients with DPNS but is less useful in the PPNS group.
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PMID:Anti-VDRL antibodies by ELISA in cerebrospinal fluid and its value in the differential diagnosis of abnormalities of the cerebrospinal fluid. 2648 30


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