Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0024141 (systemic lupus erythematosus)
 44,322 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Reduced complement-mediated solubilization (CMS) of pre-formed immune complexes (IC) was demonstrated in sera from 11 out of 12 SLE patients. The presence of incompletely solubilized endogeneous IC in SLE sera was indicated by the following findings: (1) When IC positive SLE sera with reduced CMS capacity were mixed with normal donor sera they inhibited the CMS of the latter sera. (2) Resuspended PEG (2.75%) precipitates obtained from SLE sera inhibited the CMS of normal donor sera. (3) Non-solubilized or incompletely complement solubilized IC in SLE sera give a strong response in the PEG-CC assay for IC. The IC activity of SLE sera was clearly reduced in this assay when the endogeneous IC were solubilized prior to testing. In contrast, sera of 14 rheumatoid arthritis (RA) patients exhibited normal CMS. IC which could be further solubilized by complement were not demonstrable although all RA sera were IC positive.
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PMID:Reduced complement-mediated immune complex solubilizing capacity and the presence of incompletely solubilized immune complexes in SLE sera. 665 67

Immune complexes were precipitated with polyethylene glycol (PEG) from sera of patients with rheumatoid arthritis (RA), psoriatic arthritis and systemic lupus erythematosus. Precipitates were tested for their capacity to consume complement and for the presence of fibronectin (Fn) and specific autoantibodies rheumatoid factor (RF, anti-DNA). The results showed enrichment of autoantibody activity in the precipitates. In RA, RF was especially enriched in 2.5% PEG precipitates, while IgM anti-DNA activity was more evident in 3.5% precipitates. IgG anti-DNA antibody was detected only in 3.5% precipitates from lupus sera. Complement consumption activity of precipitates was mainly related to IgM autoantibodies. There was a strong correlation between the presence of RF activity and Fn in the PEG precipitates. Nephelometric studies revealed direct interaction between the Fn and IgM RF or heat aggregated gammaglobulin. It may be possible to monitor the serum levels of immune complex material using PEG precipitation.
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PMID:Immunochemical analyses of components of immune complexes in the sera of patients with autoimmune diseases. 666 92

A method is described for quantitative measurement of C3d in plasma and synovial fluid by the use of rocket immunoelectrophoresis after fractionation of the samples with 22% polyethylene glycol. This method has the advantage over radial immunodiffusion of being more sensitive (detecting C3d down to 3 mg/l) whilst proving equally reproducible. Investigations indicate that the collection of blood samples in EDTA prevents in vitro activation of C3 even after storage for up to 6 h at room temperature and up to 12 weeks at -70 degrees C. Elevated levels of C3d were found in a proportion of SLE and RA plasma samples and in synovial fluids from patients with inflammatory synovitis. It is suggested that C3 conversion in vivo may be assessed by measurement of C3d by the technique described, and when used in conjunction with measurements of complement components and immune complexes, offers a means of investigating complement catabolism by the classical and alternative pathways.
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PMID:Measurement of the complement C3 breakdown product C3d by rocket immunoelectrophoresis. 679 79

In a prospective study, we screened sera samples of 128 patients with glomerulonephritis (GN) for the presence of circulating immune-complexes (CIC) fixing C1q by precipitation of native C1q with 2% polyethylene glycol (PEG) in the presence of 10 mM EDTA. The amount of precipitated C1q, as measured by Mancini, is less than 27% (m +/- 2 SD) of the original value in control sera. We found 35/128 (27%) positives samples, distributed as follows: 4/17 (24%) in acute GN, 10/15 (67%) in SLE, 2/16 (13%) in membraneous GN, 13/24 (54%) in membranoproliferative GN, 3/16 (19%) in segmental focal hyalinosis, 2/10 (20%) in minimal lesions nephrotic syndrome, and 1/30 (3%) in mesangial IgA GN. We then correlated these results with clinical, serological and pathological data. Whatever the type of GN, the presence of CIC fixing C1q correlated significantly, well with : the presence of chronic renal failure (serum creatinine greater than or equal to 2 mg/dl) (X2 = 5.48, p less than 0.02), the presence of hypocomplementemia (X2 = 12.30, p less than 0.001), the presence of low serum C3 (X2 = 8.25, p less than 0.01), the activation of C3 through normal pathway (low serum C4 and/or C1q) (X2 - 18.12, p less than 0.001) and the presence of glomerular deposits of C3 (X2 = 8.52, p less than 0.01), of C4 (X2 = 7.10, p less than 0.01), and of C1q (X2 = 4.11, p less than 0.05). The technique is simple, does not require labeled C1q, and allows the further study of antigen or antibody determinants of the complexes. Its sensitivity is near that of the 125 I-C1q binding assay technique. Such routine screening is a major immunopathologic step in the investigation of human GN.
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PMID:[Circulating immune complexes, detection in human glomerulonephritis by the PEG-EDTA technique (author's transl)]. 680 42

'Monospecific' antibodies directed against nuclear ribonucleoprotein (nRNP) and Smith (Sm) antigens from the sera of patients with mixed connective tissue disease (MCTD) and systemic lupus erythematosus (SLE) were used to isolate and purify the respective antigens from calf thymus nuclear extracts. The antibodies were allowed to interact with the nuclear antigens and the resulting complexes were purified by Protein-A-Sepharose and poly-U-Sepharose chromatography. When the Protein-A-Sepharose antigen-antibody complex was sequentially eluted with 20% and 60% ethylene glycol, 2 fractions could be identified, one containing antigenically active Sm and the other antigenically active Sm and nRNP. The nRNP fraction contained 5 major proteins with mol. wts ranging from 38,000 to 150,000. The Sm antigen had a mol. wt of 68,000 and was noted to 'co-purify' with the nRNP antigen. Amino acid analysis of the purified proteins demonstrated a high content of glycine, serine and acidic amino acid residues similar to previously described core proteins of heterogeneous nuclear RNP (hnRNP), a precursor to mammalian messenger RNA. Analysis of the RNA moiety following nuclease phosphorylase reactions demonstrated the presence of a RNA polynucleotide with a 'capped' structure at the 5' terminus, a feature consistent with the concept that the nRNP antigen is part of the hnRNP complex.
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PMID:The purification, characterization and amino acid analysis of nuclear ribonuclear protein and Sm antigens reacting with human autoimmune sera. 688 33

Circulating immune complexes (CIC) were measured in 4 normal controls and 8 patients with systemic lupus erythematosus (SLE) by a polyethylene glycol precipitation (PEG) method during a 24-hour period. In all the normal controls levels fell during sleep and after drinking 1 pint (568 ml) of milk and rose before getting out of bed and after moderate exercise. There were also marked differences in the patterns of rise and fall of the CIC levels between the normal controls and patients with SLE. Insufficient account has been taken of these physiological influences in CIC levels in previous studies attempting to relate disease activity to quantitative levels.
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PMID:Variation in circulating immune complex levels with diet, exercise, and sleep: a comparison between normal controls and patients with systemic lupus erythematosus. 689 50

Two patients with systemic lupus erythematosus (SLE) had pericardial tamponade. The pericardial fluid from both patients contained "mixed" type cryoglobulins consisting of IgG, IgM, and C1q. There was a selective concentration of antinuclear antibodies in the pericardial fluid cryoglobulin of one patient. Pericardial fluid from the same patient contained immune complexes. Anti-DNA antibodies were found in the serum, pericardial fluid, and pericardial fluid cryoprotein. Immune complexes isolated from the pericardial fluid by polyethylene complexes isolated from the pericardial fluid by polyethylene glycol precipitation contained antinuclear antibodies including anti-DNA antibodies. Immune complexes may be important in the pathogenesis of pericarditis in SLE.
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PMID:Immune complexes in the pericardial fluid in systemic lupus erythematosus. 696 47

The hypothesis that serum lymphocytotoxins are antigen-antibody complexes was examined. High molecular weight fractions from the sera of eighteen patients with infectious mononucleosis (IM), thirteen patients with systemic lupus erythematosus (SLE) and six healthy controls, were prepared by precipitation with polyethylene glycol 6000 (PEG). The lymphocytotoxic activity (LCA) of these PEG precipitates was significantly greater (P less than 0.01) than that of the corresponding sera and a significant correlation (r = 0.66, P less than 0.01) was observed between the LCA of sera and the PEG precipitates. In contrast to the concentration of LCA in the PEG precipitates, the heterophil antibody titres of the precipitates from IM sera were significantly less (P less than 0 05) than serum titres. Antisera raised against PEG precipitates from sera from nine patients with IM contained significant LCA. The nature of this LCA differed from that of the LCA in the original sera in temperature dependence and the molecular size. Antigen-antibody complexes in seven sera (four IM, three SLE) were dissociated at low pH (3.0) and fractionated by gel filtration at pH 3.9. The LCA of these fractions was compared with the LCA of equivalent fractions obtained by gel filtration at pH 7.2. The heterophil antibody present in sera from patients with IM and the cytotoxicity of anti-lymphocyte globulin (ALG) were used as 'antibody controls'. In this way it was shown that the LCA in patient sera, but not heterophil antibody or ALG cytotoxicity was significantly reduced (P less than 0.001) by low pH gel filtration.
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PMID:Studies of lymphocytotoxins in infectious mononucleosis and systemic lupus erythematosus: evidence for immune complex-mediated cytotoxicity. 696 48

In 30 patients with systemic lupus erythematosus the number of a circulating basophils was countered in different stages of activity. An inverse correlation was found between the absolute basophils count and anti-DNA antibodies and presumptive circulating immune complexes (as judged by polyethylene glycol precipitation of serum). A positive correlation was found between the absolute basophil count and C3 or C4 levels. IgE on the basophil surface was determined by radioimmunoassay in 7 patients. All of them showed a significantly higher surface IgE number. When the count of circulating basophils was roughly normal, 5 out of the 6 patients showed a positive basophil degranulation test with native DNA. These results suggest the existence of an anti-DNA specific IgE in lupus patients. Depression of the circulating basophil count may be a useful index of lupus activity.
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PMID:Evidence of an immediate hypersensitivity mechanism in systemic lupus erythematosus. 696 64

Serial blood samples were taken from eight patients with systemic lupus erythematosus and five normal controls before and after a standard meal containing 20 g of protein. The samples were assayed for circulating immune complexes by the fluid-phase C1q, monoclonal rheumatoid factor and conglutinin-binding assays. No significant differences were seen in the post- as compared to the pre-prandial levels of circulating immune complexes. In neither controls nor patients did circulating complexes appear for the first time within 90 min after starting the meal. We conclude that if systemic antigen--antibody reactions occur soon after a protein meal they are not detected by the polyethylene glycol-dependent tests in current use and do not appear to influence the immediate clearance of endogenous immune complexes.
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PMID:The effect of a protein meal on three fluid-phase assays for circulating immune complexes. 697 43


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