Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0024141 (systemic lupus erythematosus)
 44,322 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Sera from 98 patients were examined for antinuclear antibodies (ANA). The patient population has been previously identified clinically as having the following diseases: systemic lupus erythematosus, scleroderma, dermato or polymyositis, discoid lupus, rheumatoid arthritis, Raynaud phenomena only, undifferentiated connective tissue disease, and psoriasis. All sera samples were tested using both HEp-2 cells and rat kidney tissue as substrates and were stained with both fluorescein-conjugated antihuman antibody and glucose oxidase-conjugated antibody to human IgG. Each serum was initially tested at a screening dilution of 1:40 with PBS. Positive sera were serially diluted until an end point was observed. The number of dilutions for each specimen in all four combinations was compared mathematically using the Pearson product moment correlation. Using this method, glucose oxidase- and fluorescein-conjugated antinuclear antibody (FANA) techniques appear to have a high positive correlation (r = 0.92 kidney, r = 0.95 Hep-2) in this patient population. In our experience, the glucose oxidase technique offers comparable results to FANA and is ideally suited for the hospital laboratory, especially facilities without the benefit of a fluorescent microscope.
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PMID:A comparative study of glucose oxidase versus FITC-labeled antibody techniques for the detection of antinuclear antibodies. 643 30

Antibodies against native DNA have been evaluated by an immuno-enzymo assay (ELISA) using glucose oxidase labelled Ig class-specific antibodies. Results of the ELISA test were significantly correlated with those of the Farr test performed with native DNA as well as with the degree of clinical activity. ELISA is proposed as a simple method for the direct evaluation of class-specific anti-native DNA antibodies in systemic lupus and related diseases.
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PMID:Direct evaluation of class-specific anti-DNA antibodies by an immunoenzymatic technique. 699 57