Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0024141 (systemic lupus erythematosus)
 44,322 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

An EIA for measuring anti-TPO autoantibodies (rhTPO-EIA) was developed using recombinant human TPO expressed in CHO cells and was compared with MC-HA generally used in laboratory routine work. rhTPO-EIA showed a satisfactory reproducibility in the intra-assay test and did not have an accidental error of lots. Almost equal number of healthy females and males were measured for their IgG binding to TPO to define a normal range of anti-TPO autoantibodies. After setting 20 IU/ml as an upper limit of normal range, sera from patient with thyroid disorders were measured for their anti-TPO autoantibodies. Chronic thyroiditis and Graves' disease were highly positive, while adenoma, thyroid cancer, SLE, and RA were low in their positivity. The positive rate of anti-TPO autoantibodies was compatible to those of previous reports in each disorder. Seventy-two sera from patients with chronic thyroiditis or Graves' disease were measured for their autoantibodies by both rhTPO-EIA and MC-HA and the results were compared between both methods. A correlation coefficient was 0.486. Following absorption with thyroglobulin, sera were measured again and as the results, the correlation coefficient increased to 0.723. Therefore, MC-HA was thought to be influenced in the presence of anti-thyroglobulin autoantibodies. Since rhTPO-EIA is excellent in quality and not affected by anti-thyroglobulin antibodies, it is useful and applicable to clinical diagnosis and observation of thyroid disorders.
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PMID:[Measurement of human thyroid peroxidase autoantibodies by enzyme immunoassay using recombinant human TPO]. 815 66

TPO is a major antigen corresponding to thyroid-microsomal autoantibodies. Anti-TPO autoantibodies are very important to diagnose autoimmune thyroid disease and to estimate its clinical course. An EIA for measuring anti-TPO autoantibodies (rhTPO-EIA) was developed using recombinant human TPO expressed in CHO cells and was compared with MCHA generally used in routine laboratory work. Sera from patients with various disorders were measured for their anti-TPO autoantibodies. Chronic thyroiditis and Graves' disease were highly positive, while thyroid cancer, adenoma, SLE, and RA were low in their positivity. The positive rate of anti-TPO autoantibodies were compatible with those of previous reports of each disorder. In the comparison between rhTPO-EIA and MCHA, the correlation coefficient was 0.486. Following absorption with thyroglobulin, sera were measured again and as a result, the correlation coefficient increased to 0.723. Therefore, MCHA was thought to be influenced in the presence of anti-thyroglobulin autoantibodies. The characteristics of TPO antigen and anti-TPO autoantibodies were also summarized.
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PMID:[Clinical application of recombinant thyroid peroxidase]. 829 53