Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0024141 (systemic lupus erythematosus)
 44,322 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Immune complexe-like materials have been detected by a precipitation test with polyethylene glycol (PEG test) in the sera of 14 (93.3 p. 100) of 15 patients with active SLE, in 11 (44.0 p. 100) of 25 patients with inactive SLE, in 5 (83.3 p. 100) of 6 patients with widespread DLE and 3 (42.9 p. 100) of 7 patients with localized DLE. A good correlation was demonstrated between the value of PEG test and the complement consumption activity measured by an anticomplementary method in the cases of SLE, but it was not clear in the cases of DLE. The anticomplementary activity has been observed in the macromolecular fractions obtained by gel filtration on Sephadex G-200 of serum samples from one patient with active SLE and one patient with widespread DLE. As the value of PEG test had shown a tendency to increase with the antinuclear antibody titer if the titer was higher than 1 : 128, it is probable that the antinuclear factors were the important elements of the composition of circulating immune complexes in the cases of SLE. Contrarily, in several DLE patients, PEG test was positive despite lacking antinuclear antibody. In most cases, no significant decrease of PEG titers after DNase action was recorded, suggesting the participation of complexes containing antigens different from DNA.
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PMID:[Detection of circulating immune complexes in lupus erythematosus by precipitation test with polyethylene glycol and complement consumption test (author's transl)]. 30 93

Soluble immune complexes were detected using inhibition of antibody-dependent cell-mediated cytotoxicity (ADCC) in sera of patients with various diseases. Results were positive in 32/41 patients with rheumatoid arthritis (78%), in 27/38 systemic lupus erythematosus patients (71%), in 7/10 cutaneous lupus erythematosus patients (70%), in 6/8 mixed connective tissue disease patients (75%), in 11/26 membranous glomerulonephritis patients (42%), in 6/20 membranoproliferative glomerulonephritis patients (30%) and in 3/12 multiple sclerosis patients (25%). ADCC inhibition was compared with PEG precipitation technique and was found to be more sensitive for detecting soluble immune complexes. Various pitfalls are discussed.
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PMID:Detection of soluble immune complexes by the technique of ADCC inhibition in human diseases. 53 26

Using Laser nephelometric measurement a method is described to detect immune complexes, which seems to be suitable for routine studies as well as for specific complex characterisation. Tetanus-Antitetanus complexes are used as standard thus the result is expressed as tetanus complex units (TCU). The method is simple and permits the direct measurement of complexes in native sera. Furthermore it is possible to characterise the complexes either by the immuno-globuline class of the antibody involved or the antigen respectively. Complexes were found with this method in various frequency in all but one group of patients under study (SLE, RA, other connective tissue diseases, malignomas, myelomas, thyroiditis, Grave's disease and healthy controls). Their was a high correlation with two other common techniques to measure immune complexes, e.g. C1q-deviation and C1q-PEG precipitation.
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PMID:[Detection of circulating immune complexes using a laser-nephelometric assay (author's transl)]. 58 Dec 78

The search for circulating immune complexes (IC) by precipitation tests using polyethylene glycole was performed in a large series of normal (150 subjects) and 1200 pathological sera (over 800 patients). Increased precipitability of IgG and C4 was seen in a great percentage (80%) of pathological sera giving positive PEG precipitation without direct influence of IgG, IgM, C1q, C3 and C4 serum levels. The labeled C1q binding test gave similar results in 90 normal and 640 pathological sera. The C1q binding test could be replaced by the more direct and simple evaluation of the amount of C4 precipitated with IgG by 3.5% PEG. Positive results obtained in the three methods were particularly found in patients with diseases generally presumed to represent immune complexes diseases including acute glomerulonephritis, systemic lupus erythematosus, polyarteritis nodosa, subacute bacterial endocarditis, and acute or chronic hepatitis.
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PMID:[Detection of circulating immune complexes by three techniques using polyethylene glycol (author's transl)]. 60 Jul 49

Sera and 5% PEG-precipitates from 20 patients with IMN, 18 with MPGN, 20 with SLE, 8 with anti-GBM disease and 17 with other varieties of glomerulonephritides, 19 patients with chronic liver or intestinal diseases, as well as those from 40 healthy adults, were tested by gel prcipitation for the presence of a ubiquitous tissue antigen (UTA) and/or its corresponding antibody, previously shown to be associated with renal disorders. The antigen was detected in sera of 5 patients with IMN, one with anit-GBM disease and one with SLE. Corresponding antibodies were present in sera of 2 patients with IMN. In 4 of 5 patients with IMN and UTA was only detected if the sera were first treated with PEG (mol. wt. 6,000) which induces precipitation of antigen-antibody complexes and other high molecular weight components in serum. The UTA was not detected in renal glomeruli by immunofluorescence. The possible significance of these findings in the pathogenesis of renal diseases is discussed.
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PMID:A ubiquitous tissue antigen and its corresponding antibody in sera of patients with glomerulonephritides. 82 47

Antibodies to DNA can be found in the circulation of the majority of patients with Systemic Lupus Erythematosus (SLE). They are quite specific for this disease, which makes their detection an important diagnostic aid to the clinician. Fluctuations in the level of anti-dsDNA in an individual patient generally parallel the clinical state of that patient. Furthermore, the presence of anti-dsDNA may precede the diagnosis of SLE by more than a year. Four methods relevant for the measurement of anti-dsDNA antibodies are discussed in this paper: the ELISA, the indirect immunofluorescence test on Crithidia luciliae, the PEG assay, and the Farr assay. Each of these methods detects a part of the spectrum of anti-dsDNA antibodies present in the circulation of an individual patient. The ELISA is the most sensitive method, whereas the Farr assay is the most specific for SLE. However, with the latter method only antibodies of a relative high avidity for DNA are detected. Mild forms of SLE, where patients only have anti-dsDNA of a low avidity in their circulation, may easily be missed by this technique. Clinically, high avidity anti-dsDNA is related with the more frequent occurrence of nephritis, whereas low avidity anti-dsDNA antibodies are more often found in patients with central nervous system involvement.
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PMID:Detection of antibodies to DNA: a technical assessment. 128 79

Antibodies to DNA are quite specific for systemic lupus erythematosus (SLE) and occur in the majority of SLE patients. Therefore, their detection is an important diagnostic aid to the clinician. Detection of anti-dsDNA may precede the diagnosis of SLE by more than a year. Fluctuations in the level of anti-dsDNA in an individual patient may give important information on the clinical status of the patient. Four of the most important methods developed for the measurement of anti-dsDNA antibodies will be discussed in this paper: the Farr assay, the PEG assay, the indirect immunofluorescence test on Crithidia luciliae and the ELISA. They will also be compared with one commercially available (Farr) assay, the Amersham anti-dsDNA kit. Each method, detects a part of the spectrum of anti-dsDNA antibodies produced by a patient. The Farr assay is the most specific for SLE; however, milder forms of the disease in which patients have only low avidity anti-dsDNA may easily be missed by this technique. Clinically, high avidity anti-dsDNA is related more frequently to the occurrence of nephritis, whereas low avidity anti-dsDNA antibodies are found more often in patients with central nervous system involvement. Traditionally, SLE is considered an immune-complex disease, in which inflammatory processes are initiated by local deposition of DNA/anti-dsDNA complexes. More recently, a major role was thought to be played by crossreactions of anti-dsDNA with tissue constituents. Our current view, however, is that such a crossreactivity plays only a minor role; we postulate that binding to glomerular constituents is caused by anti-dsDNA antibodies complexed with DNA and histones.
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PMID:Anti-dsDNA: choice of assay in relation to clinical value. 175 10

Immune-complex-mediated vasculitis is a frequent complication of rheumatoid arthritis and systemic lupus erythematosus. The mechanism of deposition of immune complexes within the vessel wall in these diseases remains unknown, but probably involves other proteins. Fibronectin is a likely candidate since it possesses the ability to bind to collagen, endothelial cells, and possibly immunoglobulins and immune complexes. In this study, the binding of fibronectin to IgG and IgM cryoglobulins, cold soluble IgM, IgG, IgG subclasses and IgG fragments was investigated in the solution phase. Static light scattering, fluorescence anisotropy, fluorescence intensity, and PEG precipitation studies were used to investigate binding under different conditions of temperature and ionic strength. These studies failed to demonstrate significant binding between fibronectin and IgM, IgG, IgG subclasses and IgG fragments under the conditions studied. These findings argue against solution phase binding of fibronectin and immunoglobulins contributing to immune complex vasculitis. The possibility of important surface interactions between these proteins has not been ruled out.
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PMID:Lack of binding between cryoimmunoglobulins, immunoglobulins and fibronectin: implications for immune complex vasculitis. 182 33

In this paper we will briefly compare four assays in use in our institute for the measurement of antibodies to DNA: the anti-DNA ELISA, the PEG assay, the indirect immunofluorescence test on Crithidia luciliae and the Farr assay. Although with the use of sera from defined patients with systemic lupus erythematosus (SLE) quite good correlations were obtained between the various assays, these correlations were lost upon the use in routine screening of sera of undefined patients. It will be shown that the Farr assay has the highest specificity to systemic lupus erythematosus, whereas the ELISA and the PEG assay are the most sensitive methods. In its present form, however, the ELISA is not suited for the detection of IgM anti-DNA. Furthermore, this technique alone also detects DNA/-anti-DNA complexes present in sera or hybridoma culture supernatants.
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PMID:A comparison of assays used for the detection of antibodies to DNA. 220 95

Hybridomas producing monoclonal antibodies to DNA were prepared from NZB/W F1 (n = 20), MRL/lpr (n = 13), mice with a chronical graft versus-host-disease (GVHD) (n = 8) and polyclonally stimulated mice (n = 9). Screening was performed by means of an anti-DNA ELISA. Reaction patterns in four different anti-DNA assays (anti-DNA ELISA, indirect immunofluorescence on Crithidia luciliae, PEG assay and Farr assay) as well as avidity and cross-reactivity of these monoclonals were studied in relation to anti-DNA (sub)class and murine origin of the clones. It was found that monoclonal anti-DNA derived from mice with chronic GVHD did not differ from monoclonal anti-DNA derived from NZB/W F1 or MRL/lpr mice, with respect to isotype distribution, avidity towards DNA, cross-reactivity and assay behaviour in the anti-DNA assays mentioned before. In contrast, monoclonal anti-DNA obtained from polyclonally stimulated mice were all of the IgM isotype and displayed a stronger cross-reactive behaviour than the other three models. Altogether, these results exclude the possibility that anti-DNA in the GVHD mice originates from the non-specific pool of natural autoantibodies and further emphasize the relevance of chronic GVHD as a murine model of systemic lupus erythematosus.
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PMID:Murine monoclonal antibodies to DNA. A comparison of MRL/lpr NZB/W and chronically graft-versus-host-diseased mice. 235 56


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