Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0023890 (
cirrhosis
)
42,195
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Molecular probes activated by a single enzyme have been extensively used in bioimaging and disease diagnosis; however, imaging and identification in an accurate manner remains a challenge for such probes. Here, based on the specificity of enzyme recognition, we engineered a "double-locked" and enzyme-activated molecular probe (
NML
) for accurate bioimaging and hepatopathy differentiation. Triggered by the successive reactions with leucine aminopeptidase (
LAP
, first "key") and monoamine oxidase (MAO, second "key"), the emissive fluorophore (
NF
) was released.
NML
can be activated only in the presence of both
LAP
and MAO and can be silenced when either enzyme is inhibited. Benefiting from the "double-locked" strategy,
NML
showed higher accuracy for imaging of drug-induced liver injury (DILI) than the "single-locked" probe. With serum testing,
NML
showed significant differences in mouse models of both CCl
4
-induced
liver cirrhosis
and DILI. Significantly,
NML
can be applied to accurately distinguish serum samples from clinical patients with different hepatopathies. Our smart molecular probe may hold great potential for hepatopathy diagnosis and clinical transformation.
...
PMID:A "Double-Locked" and enzyme-activated molecular probe for accurate bioimaging and hepatopathy differentiation. 3219 Feb 49
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