Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0023890 (cirrhosis)
 42,195 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Determination of p21, a product of Ha-ras oncogene, and HBsAg in hepatocellular carcinoma (HCC) and liver cirrhosis etc. was carried out with ABC method. The results showed that HCC tissues exhibited enhancement of p21 expression with a positive rate of 72.4%, which was obviously higher than the expression of p21 in tissues from liver cirrhosis, chronic hepatitis and hepatoblastoma. The p21 positive rate of regenerative cirrhosis nodules close to the HCC was 87.2%. The p21 expression level in HBsAg positive regenerative nodules of cirrhosis close to the HCC was significantly high, and its positive rate reached 93.9%. The expression level of p21 protein in well-differentiated HCC was higher than that of poorly differentiated and undifferentiated HCC. Therefore, the result suggests that the expression level of p21 in liver cirrhosis is related to persistent infection of HBV. The elevated expression of p21 plays an important role in the development of regenerative nodules in liver cirrhosis towards HCC, and it is also an important factor in the early stage of HCC development.
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PMID:[Expression of p21 in hepatocellular carcinoma and liver cirrhosis and its relation with HBV infection]. 165 96

In this study, HBsAg, HBcAg and HBV DNA in myeloid cells of 57 patients with hepatitis have been examined by using ABC staining method. The results show that in the myeloid cells of 54 patients with positive HBVM in serum, there are 4 cases with HBV positive antigen, HBcAg has been found in a case of acute hepatitis and HBsAg in a case of chronic hepatitis and a case of liver cirrhosis respectively, HBsAg and HBcAg were found simultaneously in another case of liver cirrhosis. Nothing has been found the myeloid cells of 3 cases with negative HBVM in serum. All these findings suggest that myeloid cells are probably another breeding ground for duplicating HBV.
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PMID:[The conditions of HBV infection in myeloid cells in patients with hepatitis]. 203 91

We detected the presence and distribution of HBcAg in the liver by immunohistochemistry (ABC method) and the presence of HBV-DNA in serum (spot hybridization) and anti-HBe in serum (ELISA) from 59 cases of hepatitis B hospitalized in our hospital, including 47 cases of CAH, 5 cases of CPH, and 7 cases of subacute fulminant hepatitis. 1. HBcAg in the liver was detected in 25 out of 47 cases (53%) of CAH, in 2 out of 5 cases of CPH and in 4 out of 7 cases of subacute fulminant hepatitis. The total percentage was 53% (31/59). 2. There was no positive correlation between HBV replication activity and liver disease activity (P greater than 0.05). Our results did not support the hypothesis that suggests a direct cytopathic effect of HBV. Oppositely, the fact was that the presence, the amount and the patterns of HBcAg in the liver, and the presence of HBV-DNA in serum were predominant in mild CAH compared with those in severe CAH, predominant in CAH without cirrhosis compared with those in CAH with cirrhosis. There was a tendency of inverse correlation between HBV replication activity and liver disease activity. The results above were in line with the concept that HBcAg expressed on the surface of infected hepatocytes may be relevant target for T lymphocyte cytotoxicity. The results have suggested that an immune response to HBV is present, leading to the destruction of most infected cells. 3. There was a positive correlation between HBV-DNA in serum and HBcAg in the liver (P less than 0.005), indicating that HBV-DNA in serum can represent HBV replication.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:[Relationship between HBcAg in the liver and mechanisms of chronic type B hepatitis HBVM in serum]. 259 35

The T lymphocytes and their subsets in the peripheral blood and spleen of the patients with idiopathic portal hypertension (IPH) and liver cirrhosis were analysed using OK and Leu series monoclonal antibodies. The healthy subjects showed no difference between the OK series (OKT3, OKT4, OKT8) and the Leu series (Leu3a, Leu2a). In the patients with IPH or liver cirrhosis, the percentages of OKT3 and Leu 4 positive lymphocytes (pan T), and OKT4 and Leu3a positive lymphocytes (H/I T) were similar, but the percentages of OKT8 and Leu2a positive lymphocytes (S/C T) in the patients with IPH were slightly decreased. The changes in the T lymphocyte subsets differed among the patients, but the patients with IPH and liver cirrhosis showed a slight decrease in the mean number of H/I T and a slight increase in that of S/C T after splenectomy. Pan T and H/I T in the IPH cases were similar to those of the normal spleen, but S/C T was slightly higher than that of the normal spleen. The distribution of the lymphocytes in the spleen examined by the ABC method showed that in the normal spleen, the OKT-positive lymphocytes were concentrated around the artery. The B1-positive lymphocytes were distributed on the outer portion of the OKT3-positive lymphocytes aggregate in a mantle shape. The distribution of the OKT4-positive subset and that of the OKT3-positive subset were almost the same. OKT8-staining was seen sporadically in the medullary cord, but there were slightly more OKT8-positive lymphocytes in the medullary cord in these spleens than in the normal spleen.
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PMID:[A study of the T-lymphocytes and their subsets in the peripheral and spleen using OK and Leu series monoclonal antibodies--especially patients with idiopathic portal hypertension (IPH) and liver cirrhosis]. 350 35

The major theoretical approaches that have provided competing explanations for the distribution and nature of alcohol problems are reviewed and assessed. The sociocultural model and studies are briefly discussed with major emphasis given to the particular studies that have focused on different conceptions and dimensions of availability. Attempts to integrate the sociocultural model and the distribution of consumption model are also reviewed. A recent integrated model finds specific relationships between physical availability or the different types of outlets, with social area characteristics of different populations, and some four different alcohol problems, including cirrhosis, public drunk arrests, and misdemeanor and felony drunk driving arrests. Specific social area characteristics representing social class, minority status, and other structural features such as unemployment and women's labor force participation become implicated in alcohol problems independently of the effects of differing levels of availability. Additional related research on licensing and enforcement practices, beliefs, and attitudes of ABC personnel are also reviewed in relation to the general issue of availability. Other recent research on social and psychological dimensions of availability are also reviewed. Cross-sectional time series modeling is suggested as a technique for determining causal processes.
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PMID:The role of alcohol availability in alcohol consumption and alcohol problems. 639 May 54

Hepatitis B virus (HBV) DNA and its 5 antigens were studied in 225 cases of paraffin-embedded sections of human liver cirrhosis obtained by biopsy. HBxAg, pre-S1 and pre-S2 antigens were detected by immunohistochemical ABC method, HBsAg and HBcAg by PAP method. HBV DNA by in situ hybridization, and both HBV DNA and HBsAg, HBxAg or HBcAg by double labelling technique of immunohistochemistry and in situ hybridization respectively. The results showed that the positive rates were 70.0% (128/183) for HBsAg, 64.4% (85/132) for pre-S1 antigen, 61.4% (81/132) for pre-S2 antigen, 75.3% (113/150) for HBxAg, 22.4% (39/174) for HBcAg and 62.4% (58/93) for HBV DNA respectively. The double labelling positive rates were 37.3% (19/51) for both HBV DNA and HBsAg, 86.3% (44/51) for both HBV DNA and HBxAg and 39.2% (20/51) for both HBV DNA and HBcAg respectively. More than 80% of the cases with positive sections for HBV DNA and its 5 antigens were associated with liver cell dysplasia (LCD). The results of this study suggest that the occurrence and development of liver cirrhosis were closely related to chronic infection of HBV in China.
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PMID:[Expression and significance of HBV DNA and its 5 antigens in liver cirrhosis]. 778 Nov 8

C/EBP is a sequence-specific DNA-binding protein. In order to identify its distribution and localization, immunohistochemical technique (ABC method) was done using anti-C/EBP polypeptide antibodies 1103#, 425# in liver specimens from 20 normal adults, 5 neonates, 6 patients with hepatitis, 25 with liver cirrhosis, 80 with hepatocellular carcinoma (40 cases were associated with surrounding nontumorous tissues) and 26 patients with cholangiocarcinoma (15 cases were associated with surrounding nontumorous tissues). The results showed that C/EBP was diffusely distributed in nuclei and cytoplasm of differentiated liver cells and very low or undetectable in liver cancer cells. The manifestation of C/EBP correlated with degree of differentiation of tumour cells, and was obviously weaker than that in surrounding nontumorous tissues. C/EBP positive staining has also been found in regenerating epithelial cells of bile ductules. The results suggested that C/EBP should play an important role in establishing and maintaining the differentiation of liver cells.
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PMID:Immunohistochemical demonstration of CCAAT/enhancer binding protein (C/EBP) in human liver tissues of various origin. 780 44

Surgical specimens of 20 cases of human intrahepatic cholangiocarcinoma (n = 12) and cholangiohepatocarcinoma (n = 8) were studied immunohistochemically by ABC technique for HBxAg, pre-S1 and pre-S2 and by PAP method for HBsAg and HBcAg. The neighboring liver tissues with chronic hepatitis or cirrhosis surrounding the tumor were also examined in 19 cases. Of the cancerous tissues, 15 were positive for HBxAg (75%), 8 positive for pre-S1 and pre-S2 (40%), respectively and 2 for HBsAg (10%). Sixteen of 19 liver tissues surrounding the tumor were also positive for HBxAg (84.2%), 9 for pre-S1 and pre-S2 each (47.4%), 6 for HBsAg and HBcAg each (31.6%). The results suggest that a close relationship exists between cholangiocarcinoma and cholangiohepatocarcinoma and hepatitis B virus infection. The HBxAg might play an important role in the pathogenesis of cholangiocarcinoma.
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PMID:[Expression of five different antigens of HBV in human intrahepatic cholangiocarcinoma and cholangiohepatocarcinoma]. 817 60

C/EBP, a heat-stable DNA-binding protein, play a very important role in establishing and maintaining the differentiation state of liver cells as a transcription factor enriched in the liver. In order to identify its distribution, localization and function, immunocytochemical examination (ABC method) was done by using anti-C/EBP polypeptide antibodies 1103#, 425# in 20 normal human adult liver tissues, 5 neonatal liver tissues, 6 hepatitis tissues, 25 liver cirrhosis tissues, 80 hepatocellular carcinoma tissues (40 cases with associated surrounding non-tumor hepatic tissues) and 26 cholangiocarcinoma tissues (15 cases with associated surrounding non-tumor tissues). The results showed that C/EBP expression was restricted to terminally differentiated liver cells, and was very low or undetectable in poorly differentiated liver cells, such as liver tumor cells. Its expression also correlated with the grading of hepatocellular carcinoma. Besides, positive C/EBP stain could be found in all regenerating bile ductules. The C/EBP has a diffuse distribution which could be detected both in nuclei and in cytoplasm, but more abundant in cytoplasm. The results are in accordance with the concept that C/EBP plays an important role in establishing and maintaining the differentiation state of liver cells.
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PMID:[Determination on of C/EBP in human liver cancer, normal adult liver and various diseased liver tissues]. 817 74

In order to know whether HBV antigen exists in extrahepatic tissue, we detected HBV antigen in liver and gallbladder tissue obtained from 31 cases with HBV chronic liver disease (17 active cirrhosis, 14 chronic hepatitis) by using polyclonal antibody and ABC method. All cases were diagnosed by biopsy (n = 12) or autopsy (n = 19), 25 were males and 6 females, the average age was 45.6 years. The results showed that in the liver tissue 30 cases were (96.77%) HBsAg positive and 15 cases (48.39%) HBcAg positive. In the gallbladder tissue 18 cases (58.06%) were HBsAg positive and 8 cases (25.81%) HBcAg positive. Although 26 cases had pathological changes in the gallbladder, the changes had no relation with the existence of HBV antigen. Their symptoms and signs were also not related with the existence of HBV antigen in the gallbladder. Among the 31 cases, 19 died. The cause of death was severe hepatic complication, but not the pathological change of the gallbladder. The results suggest that pathogenesis of HBV in extrahepatic tissue needs to be elucidated.
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PMID:[Detection and significance of HBV antigen in gallbladder tissue]. 826 87


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