Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0023890 (cirrhosis)
 42,195 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Apoptosis and nuclear antigen of proliferating cells were detected by labelling technique of in situ terminal deoxynucleotide transferase and immunohistochemical method in liver cirrhosis and hepatocellular carcinoma(HCC). The density of apoptotic cells in HCC was significantly lower than that in cirrhosis, and the density of proliferating cells was much higher in HCC than that in cirrhosis. Apoptotic cells mainly distributed in the peripseudolobular region of cirrhosis and formed an apoptosis zone. But they scattered within the cancer tissue. The results suggest that the formation of apoptosis zone in cirrhosis may be related to the change of liver blood stream. Selective proliferation of cells may exist during carcinogenesis of liver cirrhosis.
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PMID:[Study on relationship between apoptosis and proliferation of cells in liver cirrhosis and hepatocellular carcinoma]. 1068 20

We evaluated the IGF1 system in cholangiocytes of primay biliary cirrhosis (PBC) patients and investigated the relationships with apoptosis. Biopsies of PBC patients (n=32) and normal subjects (n=5) were investigated by immunohistochemistry for expression in cholangiocytes of IGF1, IGF1-R, pAKT, terminal deoxynucleotide transferase end labeling (TUNEL), Bax (proapoptotic protein), and Bcl2 (antiapoptotic protein). Whereas normal cholangiocytes were almost negative, cholangiocytes of PBC patients showed strong IHC staining for IGF1, IGF1-R, and pAKT, which increases from stage I to stage IV, where >70% of cholangiocytes were positive. Bax/Bcl2 ratio reached the highest value (4.6) in PBC stage III when apoptosis is maximal (24% TUNEL positivity), whereas it declines in stage IV (1.4) when only 7.8% cholangiocytes were TUNEL positive. In PBC stages III and IV, expression of IGF1, IGF1-R, and pAKT in cholangiocytes was directly correlated with the antiapoptotic Bcl2 and inversely correlated with proapoptotic Bax, Bax/Bcl2 ratio, and TUNEL positivity. In conclusion, cholangiocytes of PBC patients showed a marked increase in IGF1, IGF1-R, and pAKT expression involving most cholangiocytes surviving in the terminal ductopenic stage. This was associated and correlated with a balance of pro- and antiapoptotic proteins favoring survival rather than apoptosis, suggesting a major role of IGF1 system in promoting cholangiocyte survival.
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PMID:Activation of the IGF1 system characterizes cholangiocyte survival during progression of primary biliary cirrhosis. 1716 8

Aflatoxin B1 (AFB1) and the hepatitis B virus X antigen (HBx) are linked to the formation of liver diseases and hepatocellular carcinoma (HCC). The aim of this study was to investigate the synergistic effects between HBx and AFB1 in causing liver disorders using a transgenic zebrafish animal model. Histopathology, Periodic acid-Schiff (PAS) staining, Sirius red staining, TdT-mediated dUTP Nick End Labeling (TUNEL) assay, immunohistochemistry, and quantitative reverse transcriptase-polymerase chain reaction (Q-RT-PCR) were used to examine the livers of the HBx transgenic fish injected with AFB1. We found that HBx and AFB1 synergistically promoted steatosis as indicated by histopathological examinations and the increased expression of lipogenic factors, enzymes, and genes related to lipid metabolism. Moreover, treatment of AFB1 in HBx transgenic fish accelerated the development of liver hyperplasia and enhanced the expression of cell cycle related genes. PCNA was co-localized with active caspase 3 protein expression in HBx zebrafish liver samples and human HBV positive HCC samples by double fluorescence immunostaining. Finally, we found that in human patients with liver disease, significant glycogen accumulated in the inflammation, cirrhosis stage, and all cases of hepatocellular and cholangiocellular carcinoma showed a moderate cytoplasmic accumulation of glycogen. Our data demonstrated a synergistic effect of AFB1 and HBx on the regulation of lipid metabolism related genes and cell cycle/division-related genes which might contribute to enhanced steatosis and hyperplasia at 5.75months.
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PMID:Hepatitis B virus X antigen and aflatoxin B1 synergistically cause hepatitis, steatosis and liver hyperplasia in transgenic zebrafish. 2349 92

Although indolent T-lymphoblastic proliferation (iT-LBP) in the extrathymic location have been shown to be a distinct clinicopathologic entity, carcinoma composed iT-LBP are rare. We retrospectively analyzed the clinicopathological features of 7 hepatic carcinoma cases with iT-LBP. There were 5 male and 2 female patients, aged from 37-54 (mean 47) years. All patients had a clinical history of chronic hepatitis B viral infection with high serum AFP level. Microscopically, these carcinomas were characterized by admixed with increased amounts of fibrous and small lymphocytes composed of regressive germinal centers. Immunohistochemically, in lymphoid tissues, some TDT+ cells were highlighted in the CD3+ area. These lymphoblasts localized predominantly between the cords of the carcinoma and interfollicular regions, diffused or only focal presented more than 50 TdT+ lymphoblasts/HPF. No EBV infection cells and T-cell antigen clonal rearrangement was detected. 3/4 cirrhotic patients developed HCC recurrence, while the 4-y survival rate was 100% in non-cirrhosis patients. It-LBP is a rare unusual proliferation and easily be misdiagnosed in HC patients. It does not seem to be associated with a specific HCC type. If HC companied with numerous small lymphocytes infiltration and showed high Ki67 index, a primary HC with iT-LBP should be considered in the lists of diagnosis.
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PMID:Hepatic carcinoma with indolent T-lymphoblastic proliferation (iT-LBP). 3225 31