Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0023473 (chronic myeloid leukemia)
 18,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The murine pim-1 gene, isolated as a locus frequently activated by proviral integration in T cell lymphomas, encodes a protein serine kinase. Although genetic evidence suggests a crucial role for this protooncogene in cell growth and transformation, very little is known about its protein product. The murine pim-1 mRNA provides alternate translational starts at a CUG codon +87-89 and an AUG codon at +339-341, in the same open reading frame (ORF), resulting in 44-kDa (397 amino acids) and 34-kDa (313 amino acids) isoforms. In this report, we demonstrate that the human PIM-1 mRNA is translated only from the single initiation methionine codon at +339-341 under cell-free conditions. Immunoblotting analyses of several human solid tumor cell lines, with highly specific antisera reveal two ubiquitously expressed isoforms (35 and 34 kDa). The estimated half-life of these proteins is shorter in the normal peripheral blood leukocytes (<5 min) than in the chronic myelogenous leukemia cells K562 (<20 min). Immunoblotting analyses of centrifugally elutriated fractions of the chronic myelogenous leukemia BV173 cells demonstrate that the levels of PIM-1 increase during the progression from early to late GI, remain high at the G1/S boundary and G2 phases of the cell cycle. The results presented here suggest a ubiquitous role for PIM-1 in progression through cell cycle.
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PMID:Ubiquitous expression and cell cycle regulation of the protein kinase PIM-1. 866 Jun 54