Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0023473 (chronic myeloid leukemia)
 18,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We encountered a 38-year-old Japanese male patient with chronic myelogenous leukemia (CML), whose bone marrow and peripheral blood cells during the chronic and blastic phases contained a complex Ph1 translocation and an extra Y chromosome [i.e., 47,XYY,t(9;22;13)(q34;q11;q14)]. A karyotypic analysis of PHA-stimulated lymphocytes showed the constitutional karyotype to be 47,XYY. Thus, it was considered that CML with a complex Ph1 translocation developed in an XYY male; such a case has not been reported, so far. A B-lymphocyte cell line with the complex Ph1 translocation was established by the procedure of Epstein-Barr virus transformation. The presence of the complex Ph1 translocation in the B-lymphocyte cell line suggests that some of the B lymphocytes in this patient originated from the CML clone.
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PMID:Chronic myelogenous leukemia with a complex Ph1 translocation in an XYY male. 660 1

D cells are lymphocytes bearing both receptors for the third complement component and the ability to form spontaneous rosettes with SRBC. We report the case of a patient with a D-cell chronic lymphatic leukemia who presented a long evolution without treatment and whose leukemic cell characteristics have been extensively studied. Cytogenetic analysis showed numerous karyotypic abnormalities among leukemic cells; all metaphases were hypodiploid and arranged in four different clones; seven marker chromosomes were present. The cells were found to bear human T-cell specific antigen, the T helper/inducer phenotype, HLA-A and HLA-B determinants, but no HLA-DR antigens. They displayed a high proliferative response to PHA and Con A, no response to PWM stimulation, and possibly the capacity of allogeneic stimulation in the mixed lymphocyte culture system. Assays for cell-mediated cytotoxicity in the CML system, and for K and NK activities were negative.
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PMID:Membrane markers, karyotypic abnormalities, ultrastructure and functional properties of lymphocytes in a case of 'D-cell' chronic lymphatic leukemia. 671 63

Serum levels of immunosuppressive acidic protein (IAP) in 105 patients with hematopoietic malignancies, there were 12 cases of acute myeloblastic leukemia, 1 acute monocytic leukemia, 13 myelomonocytic leukemia, 4 acute promyelocytic leukemia, 26 chronic myelogenous leukemia, 22 non-Hodgkin's lymphoma, 5 Hodgkin's disease, 6 adult T-cell leukemia, 5 acute lymphoblastic leukemia, 3 chronic lymphocytic leukemia, and 8 multiple myeloma. High levels of serum IAP were detected in all of the patients except chronic phase of CML, malignant lymphoma in stage I and II, and multiple myeloma. In the cases of malignant lymphoma, serum IAP levels in stage III and IV were higher with statistical significance (p less than 0.01) than those in stage I and II. Serum IAP levels in the patients with CML in blastic crisis were higher than in the chronic phase, so serum IAP levels are useful as one diagnostic parameters in blastic crisis. However, in patients with ANLL in relapse, serum IAP levels showed normal values. Serum IAP levels paralleled those of acute phase reactants such as alpha 1-acid glycoprotein , C-reactive protein, alpha 2-globulin, and alpha 1-antitrypsin, and had inverse correlations with PPD and PHA skin test.
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PMID:[Quantitative measurement and clinical analysis of serum levels of immunosuppressive acidic protein (IAP) in hematopoietic malignancies]. 673 51

T lymphocytic colony formation by peripheral lymphocytes separated by discontinuous albumin gradient centrifugation was evaluated in 8 patients with Philadelphia (Ph1)-positive chronic myeloid leukemia (CML). Colonies were obtained using a liquid-on-agar culture system recently introduced (PHA overlayer-leukocyte feeder layer assay) which has been shown to be simple and reliable. The pattern of colony growth in CML and in normal controls was similar, the peak ranging from the 4th to the 6th day. Also the morphological aspects of colonies did not differ in the two groups. Cells recovered from CML lymphocytic colonies were shown to belong to T cell lineage, as they are able to form spontaneous E-rosettes and to respond to mitogenic stimulation in vitro. In contrast, cells recovered from all other cultured fractions failed to display these properties. Cytogenetic analysis showed that T colony cells were Ph1-negative whereas the chromosome anomaly was found in nonlymphoid colonies of the same patients, thus suggesting a nonclonal origin of T lymphocytes in CML.
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PMID:Ph1-negative T lymphocytic colonies in agar cultures of peripheral blood in chronic myeloid leukemia. 679 75

14 patients with chronic myelocytic leukemia were evaluated immunologically; no difference was found in mean lymphocyte percentage and absolute number between patients and healthy subjects. 4 cases (28.5%) showed decreased percentage of T lymphocytes, while only 2 cases (14.2%) had decreased absolute T lymphocyte values. PHA transformation was decreased in 57% of the patients. Spontaneous transformation in the short-term cultures exceeded the normal range in 65% of the cases. All patients skin tested were found to be reactive. Most of the patients had defective cellular immune response in vitro, probably related with a qualitative defect in T lymphocyte subpopulations. It cannot be completely excluded that part of the observed lymphocyte depression was due to the busulfan.
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PMID:Cell-mediated immunity in chronic myelocytic leukemia. 681 Jun 24

The effects of bestatin was studied in 17 patients with acute and chronic leukemia, and three patients with malignant lymphoma with or without concomitant chemotherapy. Complete remission was obtained in eight patients with acute leukemia and in three patients with malignant lymphoma. Definite effects on both clinical and hematological findings have not been observed in patients with chronic myelogenous leukemia. The PPD and PHA skin reactions increased in about 60 percent of the patients examined. Effects of bestatin on hematological and immunological parameters were obtained at a daily dose of from 30 to 90 mg of bestatin everyday for more than 2 weeks. Bestatin appeared to be useful for the maintenance therapy of hematological malignancies. No side effects due to bestatin were detected.
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PMID:The effect of bestatin on patients with acute and chronic leukemia and malignant lymphoma. 693 Jan 19

Mononuclear blood cells were isolated from patients with different types of leukaemia and studied in vitro with regard to cell functions - adhesiveness, phagocytosis, DNA synthesis and inhibitory effects of adherent cells on non-adherent leukaemic cells and PHA stimulated lymphocytes. Cells from patients with chronic myeloid leukaemia (CML) adhered to the plastic surface of the culture dishes and showed esterase staining reactions as monocytes/macrophages. They showed a normal capacity to ingest Candida albicans, while the digestion capacity appeared reduced. Non-adherent cells from CML showed a high ability to incorporate thymidine, indicating DNA synthesis. Adhesive cells from patients with CML inhibited DNA synthesis in nonadherent CML cells and in PHA-stimulated lymphocytes to about the same extent as adhesive cells from normal donors. Blood cells from acute myeloid leukaemia and chronic lymphocytic leukaemia showed on adhesiveness and a very low spontaneous thymidine incorporation.
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PMID:Inhibitory ability of adherent blood cells from patients with chronic myeloid leukaemia on DNA-synthesis in non-adherent leukaemic cells and PHA-stimulated lymphocytes. 693 74

Human pluripotent hemopoietic progenitors (CFU-GEMM) from mixed colonies when cultured in methylcellulose or agar in the presence of erythropoietin and PHA-LCM. The observed frequency is low and varies for different individuals between 0 and 4 mixed colonies/O5 mononuclear cells. CFU-GEMM do not adhere to glass or plastic surfaces; their density is less than 1.077 g/ml, and their sedimentation velocity profile peaks at 4.5 mm/h. They do not form rosettes with sheep red blood cells. These physical parameters can be used preparatively to enrich for CFU-GEMM to facilitate assessment of their biological properties such as cycle state analysis and measurement of self-renewal capacity. Preliminary information suggests that some CFU-GEMM are capable of self-replication. Cycle state data are available for a larger number of patients with various clinical conditions. CFU-GEMM were found to be quiescent under steady-state conditions. They proliferate actively during bone marrow regeneration and in stem cell disorders like Polycythemia rubra vera or CML. These changes in cycle state activity were not reflected in numerical alterations of CFU-GEMM. It was thus concluded that the assay may be used more meaningfully to assess biological properties of human pluripotent progenitors rather than their frequency.
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PMID:Properties of human pluripotent hemopoietic progenitors. 693 20

Recently, PHA supplemented culture techniques have been introduced for growing colonies of myeloid leukemia cells. To prepare purified leukemic colony forming cell (CFC) suspensions for further studies, a discontinuous albumin density gradient separation method was applied to bone marrow and blood from patients with chronic myelocytic leukemia. It was found that the PHA-responding CFC were recovered, just as the leukocyte feeder layer stimulated CFC (Robinson CFC), from the light density fractions (1.056, 1.059 and 1.062 g/ml). Density profiles of the precursor cells forming colonies of Ph1 positive cells in the PHA-leukocyte feeder and Robinson assays appeared similar. T-lymphocyte progenitor cells, which also proliferate into colonies in the PHA-leukocyte feeder assay, were in majority harvested from the more dense fractions of the gradient. E-rosette tests and chromosome analysis were used to distinguish between leukemic and lymphocytic colonies. The density distributions of the PHA responsive leukemic CFC (Ph1 chromosome positive) and T-lymphocyte CFC (Ph1 negative) partially overlapped and a complete separation of leukemic and lymphocytic CFC was not achieved.
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PMID:Density profiles and purification of chronic myeloid leukemia cells forming colonies in the PHA-leukocyte feeder assay. 694 37

The glucocorticoid receptor (GR) quantitation by a whole-cell assay and/or cytosol technique and the in vitro sensitivity to steroids have been assessed in peripheral blood cells from normal donors and patients with chronic lymphatic leukemia (CLL), acute lymphoblastic leukemia (ALL), lymphosarcoma cell leukemia (LSCL), acute nonlymphatic leukemia (ANLL), and chronic myeloid leukemia (CML). Within the lymphoproliferative diseases, ALL cells exhibited the highest GR concentration (regardless of the method used) and the highest in vitro inhibition of spontaneous [3H]thymidine ([3H]TdR) uptake by glucocorticoids. A significant relationship between GR concentration (whole-cell assay) and in vitro sensitivity to dexamethasone was also found. On the contrary, CLL cells presented the highest sensitivity to glucocorticoids in PHA-stimulated cell cultures. Cells from the only two ALL patients who did not undergo a remission after glucocorticoid-inclusive chemotherapy had both the lowest in vitro sensitivity to dexamethasone and the lowest GR concentration with whole-cell assay. Concerning myeloid leukemia, ANLL patients had GR concentrations slightly higher than those found in the ALL group but exhibited the lowest degree of inhibition of spontaneous [3H]TdR uptake by dexamethasone (stimulatory effects occurred in some cases). CML cells exhibited an inhibition degree by in vitro glucocorticoids significantly higher than that of ANLL cells but not different from that of lymphoproliferative diseases. No clear relationship among GR pattern, in vitro cell sensitivity to glucocorticoids, and clinicohematologic parameters was observed in myeloid leukemia-bearing patients.
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PMID:Glucocorticoid receptor and in vitro sensitivity to steroid hormones in human lymphoproliferative diseases and myeloid leukemia. 694


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