Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0023473 (chronic myeloid leukemia)
 18,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Activity of 8 glycosidases (6 acid lysosomal and 2 neutral cytosolic enzymes) was estimated in lymphoid cells of 28 patients with different forms of lymphoproliferative disorders: B- and T-chronic lymphocytic leukemia (CLL), non-Hodgkins lymphoma (NHL), Sezary syndrome, hairy cell leukemia (HCL) and B- and T-acute lymphoblastic leukemia (ALL). Activity of these glycosidases was also studied in mononuclear cells and granulocytes of healthy volunteers and in immature myeloid cells of 16 patients with chronic myeloid leukemia (CML). In lymphoid cells of all the patients studied (except of ALL) the glycosidases activity was decreased as compared with that of normal mononuclear cells and immature myeloid cells. Activity of the majority enzymes studied was higher in T-lymphoid cells of patients with lymphoproliferative disorders as compared with B-cells. The highest glycosidases activity was found in ALL cells and the lowest--in CLL cells of the patients with B-lymphoid cells forms of the disease. Activities of N-acetyl-beta-D-hexosaminidase, alpha-D-mannosidase and beta-D-glucuronidase were distinctly dissimilar in cells of the patients with B-CLL, B-NHL and HCL. Estimation of these glycosidases activity in lymphoid cells may be of importance in differential diagnosis of lymphoproliferative disorders.
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PMID:[Lymphoid cell glycosidases in various forms of lymphoproliferative disorders]. 181 21

alpha-L-Fucosidase and beta-hexosaminidase isoenzyme expressions were studied in normal lymphocytes and granulocytes and their leukemic counterparts. The isoenzyme patterns were obtained by chromatofocusing on PBE 94 microcolumn coupled with automated enzyme assay. In normal and leukemic lymphoid cells, two major alpha-L-Fucosidase isoenzyme components were isolated. PMN, CML, AML and AMMoL revealed an isoenzyme pattern totally different from that seen in lymphoid cells. The two major beta-hexosaminidase forms (A and B) were isolated in normal lymphocytes and granulocytes. In all leukemias tested an high amount of an intermediate form, I, was found. The alpha-L-Fucosidase and beta-hexosaminidase isoenzyme profile may facilitate differentiation between normal and leukemic cells and between different acute leukemias.
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PMID:alpha-L-fucosidase and beta-hexosaminidase isoenzymes in human leukemia. 294 73

Activities of five acid glycosidases were examined in leukocytes of 30 healthy persons, 17 patients with chronic myeloid leukemia (CML) and 7 patients with acute myeloid leukemia (AML). In leukocytes of the patients with CML activities of alpha-D-mannosidase, N-acetyl-beta-D-hexosaminidase and beta-D-glucurodase were significantly higher (p less than 0.01) and those of alpha-D-galactosidase and alpha-D-glucosidase were somewhat higher (p less than 0.05) as compared with control leukocytes. Activities of all five glycosidases in leukocytes of patients with AML were within the normal limits. Glycosidase activities were also examined in leukocytes of three groups of patients with CML at different stages of the disease: chronic, progressive and blast crisis. All the enrymes exhibited the highest activity in the first group of patients; the activities of these enxymes in the second group were lower and those in the third group were close to normal. When CML leukocytes were fractionated using the Ficoll-verografin method, the activities of the enzymes in the interface fraction (unmatured cells) were higher than those in the bottom fraction (matured granulyocytes). These data suggest that the increased glycosidase activity in CML cells is due to the presence of the population of unmatured granulocytes which are distinct from blast cells.
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PMID:[Acid glycosidases in leukocytes of patients with chronic and acute myeloid leukemias]. 301 May 66

The isoenzyme profiles of hexosaminidase (N-acetyl-beta-D-glucosaminidase) were analyzed by isoelectric focusing on horizontal polyacrylamide thin-layer gel with special emphasis on the intermediate isoenzyme (Hex I). The expression of Hex I was examined in 87 leukemia-lymphoma cell lines, in 14 B-lymphoblastoid cell lines, in 441 cases of leukemia-lymphoma (specimens containing 80% or more tumor cells), in 22 leukemia cell lines and in 14 cases of leukemia that had been treated with phorbolesters (TPA) for induction of differentiation, and in the mononuclear cell preparations separated from peripheral blood, lymph node, thymus, bone marrow, tonsil, liver, and spleen specimens from normal donors. Hex I was detected in the leukemia cell lines arrested at early, immature or at late, mature stages of B- and T-cell differentiation, but not in cell lines blocked at intermediate stages of maturation. Most myelomonocytic leukemia cell lines and the erythroleukemia cell lines showed Hex I, whereas the B-lymphoblastoid cell lines were negative for this marker. During induction of differentiation, the expression of Hex I was lost in 13 of 15 leukemia cell lines that were originally Hex I-positive. Among the panel of the "fresh" leukemia-lymphoma cells, Hex I was found predominantly in cases of acute lymphoblastic leukemia and acute myeloblastic/monoblastic leukemia, but rarely or not at all in the mature T-, B- or myeloid malignancies. However, two out of two cases of multiple myeloma were Hex I-positive, and the Hex I expression could be induced by TPA in three of six B-cell chronic lymphocytic leukemia cases. Chronic myelocytic leukemia cells remained Hex I-negative during induction of differentiation. Hex I-positivity was not detected in the cell preparations from normal tissues, and peripheral blood indicating that the normal cellular counterpart of the Hex I-positive tumor cells are present at only low percentages within the respective cell populations. It is suggested that Hex I is a marker of early lymphoid and myeloid hematopoiesis that is no longer expressed in intermediate stages of lymphoid differentiation and in later or terminal stages of myeloid differentiation, but that is again detectable in terminally differentiated B-cells. Further studies will focus on identification and isolation of normal Hex I-positive cells.
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PMID:Occurrence of particular isoenzymes in fresh and cultured leukemia-lymphoma cells. II. Hexosaminidase I isoenzyme. 348 78