Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0023473 (chronic myeloid leukemia)
 18,916 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Cianidanol (Ci) [(+)-catechin] is a lipophilic compound which interacts with membrane lipids and affects responsiveness and function of immunocompetent cells. We therefore studied the immunomodulating properties of Ci on the proliferative response of human peripheral T-cells in one-way mixed lymphocyte reaction (1-MLR) and autologous mixed lymphocyte reaction (AMLR); on the generation of cytotoxic T-cells (Tc-cells), suppressor T-cells (Ts-cells, comprising radiosensitive as well as radioresistant suppressor T-cells) and radioresistant suppressor T-cells (rrTs) in 1-MLC; and on the cytolytic activity of Tc-cells and K-cells. In 1-MLR we observed a small stimulation of cell proliferation at Ci concentrations up to 108 microM whereas higher concentrations led to a marked suppression (100% at 435 microM). The generation of Ts-cells and rrTs-cells in 1-MLR was clearly suppressed at Ci-concentrations above 435 microM and 108 microM, respectively. The timing of Ts-cell formation was not influenced. The Tc-cell generation in 1-MLR was inhibited at high doses, and at 870 microM 59% suppression was observed. A similar dose-dependent suppressive effect of Ci was seen by testing for the cytolytic activity of ADCC-reactive K-cells and of CML-reactive Tc-cells generated in 1-MLR in the absence of Ci. At the highest concentration used (870 microM) the CML was suppressed by 45% and the ADCC by 46%. Our investigation on Ci's influence on the efferent and afferent part of immune responses in vitro demonstrated both stimulatory and inhibitory effects usually occurring at low and high concentrations, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Immunomodulating properties of cianidanol on responsiveness and function of human peripheral blood T-cells and K-cells. 297 31

Non-adherent peripheral blood mononuclear cells (NA-PBMNC) from 67 chronic myeloid leukemia (CML) patients in the first and two subsequent remissions, and 23 normal healthy donors were tested for NK and ADCC activities in short term chromium release assays using K562 and antibody-coated chicken RBCs as respective targets. CML patients in remission exhibited significantly reduced NK cytotoxicity (16. 1-19.7%) compared to normal healthy donors (47.4%). Of the patients tested, 55% exhibited NK levels below the mean percent cytotoxicity--2SD (12.5%) of normal donors (low responders), while 45% exhibited NK cytotoxicity above the 12.5% level (normal responders). On the other hand, CML patients in remission showed ADCC activity comparable to that of normal healthy donors (53.3%) irrespective of whether they belonged to normal NK responder group (55.5-65.0% ADCC) or low NK responder group (39.4-48.3% ADCC). The low or normal NK responder status of CML patients was not found to be related to either progression on the disease, or the type of drug used to bring about remission, or to the period in remission at the time of testing. In-vitro treatment of effector lymphocytes with recombinant human IFN alpha resulted in augmented of NK activity in both low and normal NK responder patients. The IFN-augmented NK activity in low responder patients however remained below the normal levels.
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PMID:Natural and antibody-dependent cellular cytotoxicity in chronic myeloid leukemia patients in remission. 345 74

Human NK activity is radiosensitive under the control of X-linked genes. We have evaluated the expression of these genes in other forms of cellular cytotoxicity. The NK radioresistant and radiosensitive phenotype is expressed in ADCC. Specific cellular cytotoxicity, generated in a MLC with a radiosensitive donor as responder, was radioresistant. NK-like activity recruited from nonadherent cells of radiosensitive subjects stimulated with allogenic cells, mitogens (PHA, Con A or PWM), or recall antigens (TT or PPD) was radioresistant. The acquisition of radioresistance was relatively rapid, beginning within 24 hr after exposure to PHA, prior to detectable proliferation. Radioresistance of MLR augmented NK-like activity was maximal 3 days after initiation of the culture. MLR augmented NK-like activity was spared by the immunosuppressive polypeptide antibiotic CsA at doses up to 1 micrometer/ml. CsA did, however, reduce acquisition of radioresistance by the NK-like activity at doses above 0.01 mu gm/ml, a concentration which does not inhibit uptake of 3H-thymidine but does reduce the level of specific CML. These data suggest that mitogens and antigens, including allogeneic cells, are recruiting radioresistant NK-like activity which can be distinguished from the radiosensitive spontaneous NK activity of the cell donor. Further, in the MLR, both radiosensitive and radioresistant NK-like activity may be recruited.
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PMID:Radioresistance of culture-induced augmented natural killer-like activity. 619 19

An immunological study about the lymphocytes of nineteen patients affected by glioblastoma has been executed by using CML and ADCC tests. Eleven healthy subjects and nine ones affected by bladder carcinoma have been studied for control. The CML test has demonstrated an increase of citotoxic activity of lymphocytes in the totality of the patients affected by glioblastoma (37,09 +/- 3,67)% versus (4,00 +/- 1,78)% of controls. The ADCC test has demonstrated diminution of citotoxic activity of lymphocytes of the patients affected by glioblastoma in comparison with controls (20,57 +/- 9,77)% versus (29,18 +/- 6,67)% of the healthy controls versus (27,66 +/- 8,51)% of bearers of bladder carcinomas.
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PMID:[In vitro cytotoxicity in the immunological study of human glioblastomas]. 730 7