Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0023418 (
leukemia
)
93,477
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Makorin-2
(
MKRN2
) is a highly conserved protein and yet its functions are largely unknown. We investigated the expression levels of
MKRN2
and RAF1 in normal and malignant hematopoietic cells, and
leukemia
cell lines. We also attempted to delineate the role of
MKRN2
in umbilical cord blood CD34+ stem/progenitor cells and K562 cell line by over-expression and inhibition of
MKRN2
through lentivirus transduction and shRNA nucleofection, respectively. Our results provided the first evidence on the ubiquitous expression of
MKRN2
in normal hematopoietic cells, embryonic stem cell lines, primary
leukemia
and leukemic cell lines of myeloid, lymphoid, erythroid and megakaryocytic lineages. The expression levels of
MKRN2
were generally higher in primary
leukemia
samples compared with those in age-matched normal BM cells. In all
leukemia
subtypes, there was no significant correlation between expression levels of
MKRN2
and RAF1. sh-
MKRN2
-silenced CD34+ cells had a significantly lower proliferation capacity and decreased levels of the early stem/progenitor subpopulation (CFU-GEMM) compared with control cultures. Over-expression of
MKRN2
in K562 cells increased cell proliferation. Our results indicated possible roles of
MKRN2
in normal and malignant hematopoiesis.
...
PMID:Ubiquitous expression of MAKORIN-2 in normal and malignant hematopoietic cells and its growth promoting activity. 2467 97
