Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0023418 (leukemia)
 93,477 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Mammalian type-C viruses contain a major internal polypeptide of about 30,000 daltons that is characterized by both intraspecies and interspecies antigenic reactivities. Radioimmunoprecipitation assays were used for measurement of this protein; the assay was based upon interspecies reactivities of the protein. As little as 5 ng of the group-specific antigen of murine leukemia virus can be measured by radioimmunoprecipitation assays, thus providing an approximate 10,000-fold increase in sensitivity over the standard immunodiffusion procedure. The type-C viruses that were recently isolated from a woolly monkey and gibbon ape each have an interspecies type-C antigenic reactivity. The primate viruses, however, could be distinguished from the type-C viruses of murine, rat, hamster, and feline origin that were more highly related to each other. The interspecies reactivity of the 30,000-dalton polypeptide is an immunological marker of the mammalian type-C viruses, since even with this sensitive assay other mammalian viruses with RNA-dependent DNA polymerase activity did not contain the type-C interspecies antigen.
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PMID:Radioimmunoassay of mammalian type-C viral proteins: interspecies antigenic reactivities of the major internal polypeptide. 450 53

Cytotoxic activities of lymphocytes and antibodies against autologous tumour cells detected by 51Cr release technique were frequent in patients with chronic granulocytic leukaemia, but infrequent in patients with blastosis of acute myeloid leukaemia. Among subjects with potentially preleukaemic haematological disorders autologous cytotoxic activity was observed only in cases of cytopenia, while samples from patients with polycythaemia vera proved to be devoid of cellular and humoral cytotoxicity. In the majority of cases the cytotoxic activity of lymphocytes and antibodies could be blocked by gp70 antigens of baboon endogenous virus (BaEV) and gibbon ape leukaemia virus (GaLV). p15(E) antigens of BaEV and GaLV showed blocking activity less frequently. Digestion by glycosidase of the carbohydrate of gp70 antigens reduced their blocking activity.
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PMID:Cellular and humoral cytotoxicity in patients with leukaemia and preleukaemia. II. Oncovirus specificity of the reaction. 608 6

We find that 12 of 14 specimens of normal human term placentas analyzed by one- or two-dimensional electrophoresis and immunoblotting contain a protein or polypeptide of approximately equal to 30,000 daltons that is antigenically cross-reactive with p30 core protein of the simian sarcoma-associated virus/gibbon ape leukemia virus primate retrovirus group and is physicochemically similar to reference murine and primate type C retrovirus p30s. This finding may lead to an understanding of endogenous type C retrovirus gene expression in humans.
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PMID:Detection and immunochemical characterization of a primate type C retrovirus-related p30 protein in normal human placentas. 609 3

Human sera contain antigens and also circulating immune complexes that are related to the primate retroviral envelope glycoprotein gp70 of simian sarcoma/simian sarcoma associated virus (SiSV) and of gibbon ape leukemia virus (GaLV). SiSVgp70 related antigens (AG) and immune complexes (IC) are detected both in leukemic and in nonleukemic sera. In a further analysis of these data, the prognostic significance of SiSVgp70 related AG and IC in leukemic patients was examined. The data show that the presence of SiSVgp70 related AG and IC indicates an unfavorable clinical course and a shorter survival time in acute leukemias (AL) and in chronic myelogenous leukemia in blast crisis (CML-BC). Survival data of 56 of 64 patients tested were analyzed (38 patients with AL and 18 patients with CML-BC). Patients with AL whose sera were positive for SiSVgp70 related AG and IC had a median survival time of 9.5 months after diagnosis versus 16 months for patients negative for such AG and IC. This difference in survival time was more pronounced for patients with acute nonlymphocytic leukemia (ANLL) (6.5 versus 19 months). The difference in survival between SiSVgp70 related AG- and IC-negative and positive groups as tested by life table analysis (log-rank test) is significant (P less than 0.05). Patients with AL of the AG- and/or IC-positive group had fewer complete remissions. Patients who had no remissions belong to the AG- and/or IC-positive group (P = 0.06). Patients with CML-BC whose sera were positive for SiSVgp70 related AG and/or IC had a median survival time of 2 months after diagnosis versus 7 months for patients with sera negative for such AG and IC. As tested by log-rank test, survival curves between the two groups are significantly different (P less than 0.05). These findings suggest that SiSVgp70 related AG and IC may play an important role in the course of acute leukemia and can provide useful prognostic information.
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PMID:Antigens and circulating immune complexes related to the primate retroviral glycoprotein SiSVgp70. Indicators of early mortality in human acute leukemias and chronic myelogenous leukemias in blast crisis. 609 85

Blood plasma samples from adult patients and children with malignant lymphoproliferative diseases were studied for presence of antibodies to primate type-C viruses by membrane immunofluorescence. Antibodies to baboon endogenous virus could be detected in various types of lymphoid leukaemias and lymphomas. Gibbon ape leukaemia virus-specific antibodies were mainly found in B- and O-cell leukaemias and lymphomas. Anti-HTLV antibodies could be detected only in a few cases of T-cell malignancy. There was no evidence of a horizontal transmission of such viruses.
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PMID:Natural antibodies to simian type-C viruses and human retrovirus HTLV in patients with lymphoid malignancies. 610 Feb 23

Leukocytes from patients with chronic myelogenous leukaemia were examined for antigens related to the p30 polypeptides of baboon endogenous virus (BaEV) and gibbon ape leukaemia virus (GaLV). Samples from patients with the quiescent phase of the disease proved to be negative or contained p30 of BaEV as the only viral antigen. In cases of blastoid crisis or acceleration, an antigen related to p30 of GaLV could be detected. In 5 of 6 patients, acceleration or blastoid crisis was preceded by expression of GaLV p30.
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PMID:Detection by radioimmunoassay of antigens related to the P30 polypeptides of primate type C oncoviruses in peripheral leukocytes from patients with chronic myelogenous leukaemia. 612 Jun 39

Antibodies reacting with gp70 and p15 antigens of baboon endogenous virus (BaEV) and gibbon ape leukaemia virus (GaLV) were detected by radioimmunoprecipitation (RIP) in blood plasma samples of patients with chronic granulocytic leukaemia, acute myeloid leukaemia and with potentially preleukaemic haematological disorders. Anti-gp70 antibodies were found more frequently than anti-p15 antibodies. Digestion of the carbohydrate part of gp70 antigens by glycosidase treatment abrogated the precipitation mediated by IgM antibodies, whereas that mediated by IgG antibodies was not markedly affected. Data suggest that antibodies detected in human plasma samples may have oncovirus specificity, but in considerable part of cases they can be of heterophil nature.
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PMID:Studies on the specificity of human antibodies reacting with GP70 and P15 antigens of baboon endogenous (BaEV) and gibbon ape leukaemia (GaLV) viruses. 614 94

The present study describes the separation and purification of a reverse transciptiase from an orbital tumor of a patient with acute myelomonocytic leukemia. Specific reaction conditions with respect to ionic requirements and template-primers are reported. The purified enzyme was able to transcribe (rA)n . (dT)12, (rC)n . (dG)12, (OMeC)n . (dg)12 and the 70 S RNA from R(Mu)LV. Serological studies that the reverase transcriptase is antigenically related to reverse transcriptase from the type C woolly monkey virus-gibbon ape leukemia virus group.
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PMID:RNA-dependent DNA polymerase activity in ocular granulocytic sarcoma associated to acute myelomonocytic leukemia in Turkish children. Biochemical and immunological characterization of the enzyme. 615 28

The biological and antigenic properties of HEL-12 virus have been compared with gibbon ape lymphosarcoma virus (GALV) and simian sarcoma and simian sarcoma-associated viruses, SiSV and SSAV, respectively, HEL-12 virus did not transform human or marmoset fibroblasts but rescued SiSV focus-forming activity from non-productively transformed marmoset cells (HF/SiSV-NP). Like SSAV and GALV, HEL-12 virus induced syncytia with XC cells. In addition, HEL-12 cells which did not produce virus but which contained HEL-12 proviral DNA, rescued SiSV from HF/SiSV-NP cells in co-cultivation experiments. Results of neutralization and serum cytotoxicity tests utilizing SiSV rescued by HEL-12 [SiSV-(HEL-12)] indicated that HEL-12 virus envelope proteins are very closely related to those of SSAV but readily distinguished from those of GALV. Antigenic diversity of SiSV(SSAV), SiSV(GALV) and SiSV(HEL-12) envelope glycoproteins (gp70) was shown in competition radioimmunoassays (RIA) designed to detect minor antigenic differences using antiserum monospecific for SiSV(SSAV) gp70 or Friend murine leukaemia virus gp70. Antigenic differences between these gp70s were demonstrated in RIA using purified SSAV gp70 or HEL-12 gp70. These data indicate that HEL-12 virus has biological properties similar to those of SSAV and GALV, is distinguished from GALV in neutralization tests and has both distinct and SSAV-related gp70 antigenic determinants.
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PMID:Biological and antigenic characteristics of HEL-12 virus. 616 87

Tissues obtained at necropsy from a 7 year old male gibbon ape with malignant lymphoma and leukemia were analyzed by electron microscopic, immunological and enzymological techniques to determine the comparative localization of tumor cells and virus throughout the body. In general, the different assays correlated well; the reverse transcriptase (RT) assay and p30 radioimmunoassay (RIA) being the most sensitive, although the RT assay was able to detect activity in one tissue scored negative by p30 RIA. Tissues were infiltrated with tumor cells to varying degrees which correlated well with the level of virus markers in most cases with the exception of the liver and kidney. In these 2 organs there was marked infiltration of free virus and tumor cells but there was no evidence of virus infection or production by these cells.
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PMID:Comparison of the tissue distribution of reverse transcriptase, p30 and type-C virus in a gibbon ape with lymphocytic leukemia. 618 17


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