Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0023418 (leukemia)
 93,477 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Interleukin 12 (IL-12: natural killer cell stimulatory factor, NKSF; cytotoxic lymphocyte maturation factor, CLMF) was studied for its effect on colony formation and lineage expression of low-density bone marrow cells from 5-fluorouracil-treated mice, and of sorted stem cells using a semi-solid culture assay in the absence or presence of IL-3, IL-11, Steel factor (SF) and erythropoietin. IL-12 did not support colony formation as a single factor, nor in the presence of IL-11 or SF. In IL-3-containing cultures, IL-12 slightly enhanced neutrophilic and monocyte differentiation. Both SF and IL-11 synergized with IL-3 to increase the percentage of multilineage colonies and the number of colonies containing erythrocytes, megakaryocytes, neutrophils, eosinophils, monocytes/macrophages, and blast cells, but not mast cells. In the presence of IL-3 + IL-11, IL-12 greatly enhanced neutrophil, megakaryocyte, erythrocyte, and mast cell development. In IL-3 + SF-containing cultures, IL-12 further increased colony numbers and a higher percentage of colonies expressed neutrophilic, megakaryocytic, erythroid, monocytic, blast cell, and/or mast cell lineages. Colony size and the presence of eosinophils in colonies were unaffected by IL-12 addition. These effects of IL-12 could not be reversed by antibodies against interferon-gamma. Our data show that IL-12 may act as a synergistic factor, stimulating multilineage expression of hemopoietic stem cells, probably via a direct action. The observed activity of IL-12, however, required the presence of a least two factors, i.e. either IL-3 + IL-11, or IL-3 + SF.
Leukemia 1993 Sep
PMID:Interleukin-12 enhances interleukin-3 dependent multilineage hematopoietic colony formation stimulated by interleukin-11 or steel factor. 769 Apr 39

Interleukin 12 (IL-12; natural killer cell stimulatory factor, NKSF; cytotoxic lymphocyte maturation factor, CLMF) was studied for its effects on the survival and generation of hemopoietic progenitors (CFU-C, CAFC day 7-14) and long-term culture initiating stem cells (CAFC day 28-35) by post-5-fluorouracil (FU) bone marrow cells (BM) in low-serum liquid culture. Previous data have shown that IL-12 may act as a potent synergistic factor stimulating multilineage expression of hemopoietic stem cells in the presence of IL-3 in combination with Steel factor (SF) or IL-11. IL-12 or IL-11 alone did not support CFU-C production in liquid culture of a low-density fraction of day-6 post-FU BM. IL-11 and SF showed potent synergy with IL-3 to augment output CFU-C numbers and support CAFC-28/35 maintenance; SF as a single factor, or in combination with IL-11 or IL-12, allowed low recovery of CFU-C and all CAFC subsets. IL-12 synergized with IL-3, and with IL-3 + SF, to moderately increase progenitor production in liquid cultures. Similarly, survival and generation of the primitive CAFC-28/35 was consistently increased when IL-12 was used in combination with IL-3, or IL-3 + SF. The combination of 3, SF and 12 supported a significantly higher recovery of CAFC-35 than did the combination of 3, SF and 11, and CAFC-35 were numerically expanded during culture. Furthermore, IL-12 acted synergistically with IL-3 to enhance CFU-C output over a prolonged period using progenitor-depleted 3-day post-FU BM. These data show that IL-12 interacts with IL-3 and SF to regulate survival and growth of myeloid stem cells and progenitor cells.
Leukemia 1993 Sep
PMID:Interleukin-12 synergizes with interleukin-3 and steel factor to enhance recovery of murine hemopoietic stem cells in liquid culture. 769 Apr 40